Substituted piperidine compounds and their use as orexin receptor modulators

ABSTRACT

The present invention is directed to compounds of Formula I: wherein X is CR 1  or N; Y is CR 2  or N; Z is NH or O; R 1  is alkoxy, triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl; R 2  is H, alkyl, or halo; R 3  is H, alkyl, alkoxy, halo, triazolyl, oxazolyl, or pyrimidinyl; R 4  is alkyl; R 5  is pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl; quinoxalinyl; pyrazinyl; or quinazolinyl; wherein the pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl; quinoxalinyl; pyrazinyl; or quinazolinyl is optionally substituted with one or two substituents independently selected from the group consisting of alkyl, halo, or phenyl; and R 6  is H or alkyl. Methods of making the compounds of Formula I are also described. The invention also relates to pharmaceutical compositions comprising compounds of Formula I. Methods using the compounds of the invention are also within the scope of the invention.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is the National Stage of International Application No.PCT/US2014/024284, filed Mar. 12, 2014, which claims the benefit of U.S.Provisional Application No. 61/780,302, filed Mar. 13, 2013, which isincorporated herein by reference in its entirety.

TECHNICAL FIELD

The present invention is directed to substituted piperidine compounds,pharmaceutical compositions comprising them, methods of making them, andmethods of using them for the modulation of the orexin receptor for thetreatment of disease states, disorders, and conditions mediated byorexin receptor activity.

BACKGROUND

Orexin/hypocretin signaling is mediated by two receptors and two peptideagonists. The peptides (orexin-A and orexin-B) are cleavage products ofthe same gene, pre-pro orexin. In the central nervous system, neuronsproducing pre-pro orexin are found solely in the perifornical nucleus,the dorsal hypothalamus, and the lateral hypothalamus (Peyron et al.,1998, J. Neurosci. 18: 9996-10015). Orexigenic cells in these regionsproject to many areas of the brain, extending rostrally to the olfactorybulbs and caudally to the spinal cord (Van den Pol, 1999, J. Neurosci.19: 3171-3182).

The orexins bind to two high affinity receptors, referred to as orexin-1and orexin-2 receptors. Orexin-1 and orexin-2 receptors areG-protein-coupled, seven transmembrane receptors that share over 64%amino acid sequence identity with one another. Both receptors aregenerally excitatory, the common cellular response to orexin-inducedreceptor activation being increases in intracellular calcium. Homologybetween the species orthologs is high and there are no knownpharmacological differences. Orexin-A and -B are usually consideredequal ligands for orexin-2 receptor but orexin-B is reported to be 5- to100-fold weaker ligand than orexin-A at the orexin-1 receptor (Sakuraiet al., 1998, Cell 92: 573-585; Ammoun et al., 2003, J. Pharmacol. Exp.Ther. 305: 507-514).

Many regions of the brain have fairly selective expression of theorexin-1 or orexin-2 receptors (Marcus et al., 2001, J. Comp Neurology435, 6-25; Trivedi et al., 1998, FEBS Letters, 438, 71-75). Orexin-1receptors are relatively selective for the limbic system (bed nucleus ofthe stria terminalis and amygdala), cingulate cortex and noradrenergicneurons in the locus coeruleus. Conversely, the orexin-2 receptor isalmost the exclusive orexin receptor in the histaminergic neurons in thetuberomammilary nucleus which play a critical role in wake promotion; inparaventricular neurons and the parabrachial nucleus. In other brainregions like the dorsal raphe, the ventral tegmental area or theprefontal cortex both receptors are coexpressed.

The broad CNS distribution of cells producing orexin, as well as cellsexpressing the orexin receptors, suggests involvement of orexin in anumber of physiological functions, including feeding and metabolism,regulation of wakefulness and sleep, sympathetic activation and stressresponse (de Lecea, 2012, Progress in Brain Research, 198, 15-24;Kukkonen, 2013, Am J. Physiol. Cell Physiol., 304, C2-C32). Orexin alsoplays a key role regulating motivation and reward associated with foodintake and with drugs of abuse (Mahler et al., 2012, Progress in BrainResearch, 198, 79-121).

Several lines of evidence indicate that the orexin system is animportant modulator of arousal. Rodents administered orexinintracerebroventricularly spend more time awake (Piper et al., 2000, J.Neurosci. 12: 726-730. Orexin-mediated effects on arousal have beenlinked to orexin neuronal projections to histaminergic neurons in thetuberomammillary nucleus (Yamanaka et al., 2002, Biochem. Biophys. Res.Comm. 290: 1237-1245). Rodents whose pre-pro orexin gene has beenknocked out, or whose orexigenic neurons have been killed, displayaltered sleep/wake cycles similar to narcolepsy (Chemelli et al., 1999,Cell 98: 437-451; Hara et al., 2001, Neuron 30: 345-354). Dog models ofnarcolepsy have been shown to have mutant or non-functional orexin-2receptors (Lin et al., 1999, Cell 98: 365-376). Orexin signaling as atarget for sleep-promoting therapies was further validated clinically byfindings of attenuated orexin levels and loss of orexinergic neurons inhuman narcoleptic patients (Mignot et al., 2001, Am. J. Hum. Genet. 68:686-699; Minot & Thorsby, 2001, New England J. Med. 344: 692) (Peyron etal., 2000, Nature Med. 6: 991-997). Disorders of the sleep-wake cycleare therefore likely targets for orexin-2 receptor modulator activity.Examples of sleep-wake disorders that may be treated by agonists orother modulators that up-regulate orexin-2 receptor-mediated processesinclude narcolepsy, jet lag (sleepiness) and sleep disorders secondaryto neurological disorders such as depression. Examples of disorders thatmay be treated by antagonists or other modulators that down-regulateorexin-2 receptor-mediated processes include insomnia, restless legsyndrome, jet lag (wakefulness) and sleep disorders secondary toneurological disorders such as mania, schizophrenia, pain syndromes andthe like.

Evidence has accumulated to demonstrate a clear involvement of orexinsignaling in reward pathways associated with drug dependence (Mahler etal., 2012, Progress in Brain Research, 198, 79-121). Orexinergic neuronssend projections to the ventral tegmental area and other brain regionsinvolved in reward processing. Orexin ligands mediate reward behavior,and antagonizing these effects with a selective orexin-1 receptorantagonist in various preclinical model of addiction has suggested thatthese actions are mediated through orexin-1 receptor. Specifically, aselective orexin-1 antagonist attenuates morphine conditioned placepreference and reinstatement (Harris et al., 2005, Nature, 437,556-5599; Narita et al., 2006, J Neurosci., 26, 398-405; Harris et al.,2007, Behav Brain Res, 183, 43-51), stress-induced cocainereinstatement, cocaine-induced behavioral and synaptic plasticity(Borgland et al., 2006, Neuron, 49, 589-601), and intake and cue andstress-induced reinstatement of ethanol (Lawrence et al., 2006, Br JPharmacol, 148, 752-759), in addition to attenuating precipitatedmorphine withdrawal (Sharf et al., 2008, Biol Psychiatry, 64, 175-183)and nicotine self-administration (Hollander et al., 2008, Proc Natl AcadSci USA., 105, 19480-19485). Another recent study has also suggested arole for the orexin-2 receptor (Shoblock et al., 2011,Psychopharmacology, 215, 191-203).

Orexin's role in more complex emotional behavior is also emerging(Johnson et al., 2012, Progress in Brain Research, 198, 133-161).Changes in orexin levels in patients with panic and posttraumatic stressdisorders have been noted (Johnson et al., 2010, Nature Medicine, 16,111-115; Fortuyn et al., 2010, General Hospital Psychiatry, 32, 49-56;Strawn et al., 2010, Psychoneuroendocrinology, 35, 1001-1007). Lactateinfusion or acute hypercapnia, which causes panic in humans, and areused as an animal model of panic, activates orexin neurons in theperifornical hypothalamus. This activation correlates with anxiety inthe social interaction test or open field test. Blocking orexinsignaling with either siRNA or selective orexin-1 receptor antagonistsattenuates panic-like responses to lactate (Johnson et al., 2010, NatureMedicine, 16, 111-115; Johnson et al., 2012, Neuropsychopharmacology,37, 1911, 1922).

Cerebral spinal fluid (CSF) levels of orexin are lower in depressed orsuicidal patients, and the level of orexin inversely correlates withillness severity (Brundin et al., 2007, EuropeanNeuropsychopharmacology, 17, 573-579; Salomon et al., 2003, BiolPsychiatry, 54, 96-104). A positive correlation between orexin-1receptor mRNA in the amygdala and depressive behavior in the forced swimtest in mice has been reported (Arendt, 2013, Behavioral Neuroscience,127, 86-94).

The orexin system also interacts with brain dopamine systems.Intracerebroventricular injections of orexin in mice increase locomotoractivity, grooming and stereotypy; these behavioral effects are reversedby administration of D2 dopamine receptor antagonists (Nakamura et al.,2000, Brain Res. 873: 181-187). Therefore, orexin receptor modulatorsmay be useful to treat various neurological disorders; e.g., agonists orup-regulators to treat catatonia, antagonists or down-regulators totreat Parkinson's disease, Tourette's syndrome, anxiety, delerium anddementias.

Orexins and their receptors have been found in both the myenteric andsubmucosal plexus of the enteric nervous system, where orexins have beenshown to increase motility in vitro (Kirchgessner & Liu, 1999, Neuron24: 941-951) and to stimulate gastric acid secretion in vitro (Takahashiet al., 1999, Biochem. Biophys. Res. Comm. 254: 623-627). Orexin effectson the gut may be driven by a projection via the vagus nerve (van denPol, 1999, supra), as vagotomy or atropine prevent the effect of anintracerebroventricular injection of orexin on gastric acid secretion(Takahashi et al., 1999, supra). Orexin receptor antagonists or otherdown-regulators of orexin receptor-mediated systems are thereforepotential treatments for ulcers, irritable bowel syndrome, diarrhea andgastroesophageal reflux.

Body weight may also be affected by orexin-mediated regulation ofappetite and metabolism. Some effects of orexin on metabolism andappetite may be mediated in the gut, where, as mentioned, orexins altergastric motility and gastric acid secretion. Orexin antagoniststherefore are likely to be useful in treatment of overweight or obesityand conditions related to overweight or obesity, such as insulinresistance/type II diabetes, hyperlipidemia, gallstones, angina,hypertension, breathlessness, tachycardia, infertility, sleep apnea,back and joint pain, varicose veins and osteoarthritis. Conversely,orexin agonists are likely to be useful in treatment of underweight andrelated conditions such as hypotension, bradycardia, ammenorrhea andrelated infertility, and eating disorders such as anorexia and bulimia.

Intracerebroventricularly administered orexins have been shown toincrease mean arterial pressure and heart rate in freely moving (awake)animals (Samson et al., 1999, Brain Res. 831: 248-253; Shirasaka et al.,1999, Am. J. Physiol. 277: R1780-R1785) and in urethane-anesthetizedanimals (Chen et al., 2000, Am. J. Physiol. 278: R692-R697), withsimilar results. Orexin receptor agonists may therefore be candidatesfor treatment of hypotension, bradycardia and heart failure relatedthereto, while orexin receptor antagonists may be useful for treatmentof hypertension, tachycardia and other arrhythmias, angina pectoris andacute heart failure.

From the foregoing discussion, it can be seen that the identification oforexin receptor modulators, will be of great advantage in thedevelopment of therapeutic agents for the treatment of a wide variety ofdisorders that are mediated through these receptor systems.

SUMMARY

The present invention is directed to compounds of Formula I:

wherein X is CR₁ or N; Y is CR₂ or N; Z is NH, N—CH₃, or O; R₁ is H,alkoxy, triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl,wherein triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl isoptionally substituted with up to two substituents selected from haloand alkyl; R₂ is H, alkyl, or halo; R₃ is H, alkyl, alkoxy, halo,triazolyl, oxazolyl, or pyrimidinyl, wherein triazolyl, oxazolyl, orpyrimidinyl are optionally substituted with up to two substituentsselected from halo and alkyl; R₄ is alkyl; R₅ is pyridyl; benzoxazolyl;pyrimidinyl; pyridazinyl; quinoxalinyl; pyrazinyl; thiadiazolyl; orquinazolinyl; wherein the pyridyl; benzoxazolyl; pyrimidinyl;pyridazinyl; thiadiazolyl, quinoxalinyl; pyrazinyl; or quinazolinyl isoptionally substituted with one or two substituents independentlyselected from the group consisting of alkyl, halo, and phenyl; and R₆ isH or alkyl. Enantiomers and diastereomers of the compounds of Formula Iare also described, as well as the pharmaceutically acceptable salts.

Methods of making the compounds of Formula I are also described. Theinvention also relates to pharmaceutical compositions comprisingtherapeutically effective amounts of compounds of Formula I. Methods ofusing the compounds of the invention are also within the scope of theinvention.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 depicts a Powder X-Ray Diffraction (PXRD) pattern for oneembodiment of the invention, Example 108.

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

The invention may be more fully appreciated by reference to thefollowing description, including the following glossary of terms and theconcluding examples.

The term “alkyl” refers to a straight- or branched-chain alkyl grouphaving from 1 to 12 carbon atoms in the chain. In some embodiments, analkyl group is a C₁-C₆ alkyl group. In some embodiments, an alkyl groupis a C₁-C₄ alkyl group. Examples of alkyl groups include methyl (Me)ethyl (Et), n-propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl(tBu), pentyl, isopentyl, tert-pentyl, hexyl, isohexyl, and groups thatin light of the ordinary skill in the art and the teachings providedherein would be considered equivalent to any one of the foregoingexamples. Alkyl groups of the invention can be substituted with, forexample, halogen atoms. One exemplary substitutent is fluoro. Preferredsubstituted alkyl groups of the invention include trihalogenated alkylgroups such as trifluoromethyl groups.

Alkyl groups of the invention can also refer to “cycloalkyl” moieties.Cycloalkyl refers to monocyclic, non-aromatic hydrocarbon groups havingfrom 3 to 7 carbon atoms. Examples of cycloalkyl groups include, forexample, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl,1-methylcyclopropyl, 2-methylcyclopentyl, and the like.

The term “alkoxy” includes a straight chain or branched alkyl group witha terminal oxygen linking the alkyl group to the rest of the molecule.In some embodiments, an alkoxy group is a C₁-C₆ alkoxy group. In someembodiments, an alkoxy group is a C₁-C₄ alkoxy group. Alkoxy includesmethoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, pentoxy and soon.

The term “benzoxazolyl” represents the following moiety:

The benoxazolyl moiety can be attached through any one of the 2-, 4-,5-, 6-, or 7-position carbon atoms.

The term “halogen” represents chlorine, fluorine, bromine, or iodine.The term “halo” represents chloro, fluoro, bromo, or iodo.

The term “isoxazolyl” represents the following moiety:

The isoxazolyl moiety can be attached through any one of the 3-, 4-, or5-position carbon atoms. Isoxazolyl groups of the invention can beoptionally substituted with, for example, one or two alkyl groups, forexample, one or two methyl groups.

The term “oxazolyl” represents the following moiety:

The oxazolyl moiety can be attached through any one of the carbon atoms.

The term “oxadiazolyl” represents a 1,2,3-oxadiazole, 1,2,4-oxadiazole,1,2,5-oxadiazole, or 1,3,4-oxadiazole moiety:

The oxadiazolyl moieties can be attached through any one of the carbonor nitrogen atoms. Within the scope of the invention, “oxadiazolyl”groups can be substituted with an alkyl group, preferably a methylgroup.

The term “phenyl” represents the following moiety:

and may be substituted with for example, alkyl or halo groups.

The term “pyridyl” represents the following moiety:

The pyridyl moiety can be attached through any one of the 2-, 3-, 4-,5-, or 6-position carbon atoms and may be substituted with for example,alkyl or halo groups, for example methyl or trifluoromethyl or fluorine.

The term “pyrimidinyl” represents the following moiety:

The pyrimidinyl moiety can be attached through any one of the 2-, 4-,5-, or 6-position carbon atoms. Within the scope of the invention,“pyrimidinyl” groups of the invention can be substituted with halogen,for example fluoro.

The term “pyrazinyl” represents the following moiety:

The pyrazinyl moiety can be attached through any one of the 2-, 3-, 5-,or 6-position carbon atoms and may be substituted with for example,alkyl or halo groups, for example methyl or trifluoromethyl or fluorine.

The term “pyridazinyl” represents the following moiety:

The pyridazinyl moiety can be attached through any one of the 3-, 4-,5-, or 6-position carbon atoms and may be substituted with for example,alkyl or halo groups, for example methyl or trifluoromethyl or fluorine.

The term “pyrazolyl” represents the following moiety:

The pyrazolyl moiety can be attached through any one of the 1-, 2-, 3-,4-, or 5-position carbon atoms. Pyrazolyl groups of the invention can beoptionally substituted with, for example, one or two alkyl groups, forexample, one or two methyl groups.

The term “quinoxalinyl” represents the following moiety:

The quinoxalinyl moiety can be attached through any one of the 2-, 3-,5-, 6-, 7-, or 8-position carbon atoms and may be substituted with forexample, alkyl or halo groups, for example methyl or trifluoromethyl orfluorine.

The term “quinazolinyl” represents the following moiety:

The quinoxalinyl moiety can be attached through any one of the 2-, 4-,5-, 6-, 7-, or 8-position carbon atoms and may be substituted with forexample, alkyl or halo groups, for example methyl or trifluoromethyl orfluorine.

The term “triazolyl” represents a 1,2,3-triazole or a 1,2,4-triazolemoiety:

The triazolyl moieties can be attached through any one of their atoms.

The term “thiadiazolyl” represents a 1,2,3-thiadiazole,1,2,4-thiadiazole, 1,2,5-thiadiazole, or 1,3,4-thiadiazole moiety:

The thiadiazolyl moieties can be attached through any one of the carbonor nitrogen atoms. Within the scope of the invention, “thiadiazolyl”groups can be substituted with an alkyl group, preferably a methylgroup.

“Pharmaceutically acceptable” means approved or approvable by aregulatory agency of the Federal or a state government or thecorresponding agency in countries other than the United States, or thatis listed in the U.S. Pharmacopoeia or other generally recognizedpharmacopoeia for use in animals, and more particularly, in humans.

“Pharmaceutically acceptable salt” refers to a salt of a compound of theinvention that is pharmaceutically acceptable and that possesses thedesired pharmacological activity of the parent compound. In particular,such salts are non-toxic may be inorganic or organic acid addition saltsand base addition salts. Specifically, such salts include: (1) acidaddition salts, formed with inorganic acids such as hydrochloric acid,hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and thelike; or formed with organic acids such as acetic acid, propionic acid,hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid,lactic acid, malonic acid, succinic acid, malic acid, maleic acid,fumaric acid, tartaric acid, citric acid, benzoic acid,3-(4-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid,methanesulfonic acid, ethanesulfonic acid, 1,2-ethane-disulfonic acid,2-hydroxyethanesulfonic acid, benzenesulfonic acid,4-chlorobenzenesulfonic acid, 2-naphthalenesulfonic acid,4-toluenesulfonic acid, camphorsulfonic acid,4-methylbicyclo[2.2.2]-oct-2-ene-1-carboxylic acid, glucoheptonic acid,3-phenylpropionic acid, trimethylacetic acid, tertiary butylacetic acid,lauryl sulfuric acid, gluconic acid, glutamic acid, hydroxynaphthoicacid, salicylic acid, stearic acid, muconic acid, and the like; or (2)salts formed when an acidic proton present in the parent compound eitheris replaced by a metal ion, e.g., an alkali metal ion, an alkaline earthion, or an aluminum ion; or coordinates with an organic base such asethanolamine, diethanolamine, triethanolamine, N-methylglucamine and thelike. Salts further include, by way of example only, sodium, potassium,calcium, magnesium, ammonium, tetraalkylammonium, and the like; and whenthe compound contains a basic functionality, salts of non toxic organicor inorganic acids, such as hydrochloride, hydrobromide, tartrate,mesylate, acetate, maleate, oxalate and the like.

“Pharmaceutically acceptable vehicle” refers to a diluent, adjuvant,excipient or carrier with which a compound of the invention isadministered. A “pharmaceutically acceptable excipient” refers to asubstance that is non-toxic, biologically tolerable, and otherwisebiologically suitable for administration to a subject, such as an inertsubstance, added to a pharmacological composition or otherwise used as avehicle, carrier, or diluent to facilitate administration of a agent andthat is compatible therewith. Examples of excipients include calciumcarbonate, calcium phosphate, various sugars and types of starch,cellulose derivatives, gelatin, vegetable oils, and polyethyleneglycols.

“Subject” includes humans. The terms “human,” “patient,” and “subject”are used interchangeably herein.

“Treating” or “treatment” of any disease or disorder refers, in oneembodiment, to ameliorating the disease or disorder (i.e., arresting orreducing the development of the disease or at least one of the clinicalsymptoms thereof). In another embodiment “treating” or “treatment”refers to ameliorating at least one physical parameter, which may not bediscernible by the subject. In yet another embodiment, “treating” or“treatment” refers to modulating the disease or disorder, eitherphysically, (e.g., stabilization of a discernible symptom),physiologically, (e.g., stabilization of a physical parameter), or both.In yet another embodiment, “treating” or “treatment” refers to delayingthe onset of the disease or disorder.

In treatment methods according to the invention, a therapeuticallyeffective amount of a pharmaceutical agent according to the invention isadministered to a subject suffering from or diagnosed as having such adisease, disorder, or condition. A “therapeutically effective amount”means an amount or dose sufficient to generally bring about the desiredtherapeutic or prophylactic benefit in patients in need of suchtreatment for the designated disease, disorder, or condition. Effectiveamounts or doses of the compounds of the present invention may beascertained by routine methods such as modeling, dose escalation studiesor clinical trials, and by taking into consideration routine factors,e.g., the mode or route of administration or drug delivery, thepharmacokinetics of the compound, the severity and course of thedisease, disorder, or condition, the subject's previous or ongoingtherapy, the subject's health status and response to drugs, and thejudgment of the treating physician. An example of a dose is in the rangeof from about 0.001 to about 200 mg of compound per kg of subject's bodyweight per day, preferably about 0.05 to 100 mg/kg/day, or about 1 to 35mg/kg/day, in single or divided dosage units (e.g., BID, TID, QID). Fora 70-kg human, an illustrative range for a suitable dosage amount isfrom about 0.05 to about 7 g/day, or about 0.2 to about 2.5 g/day.

“Compounds of the present invention,” and equivalent expressions, aremeant to embrace compounds of the Formula (I) as described herein, whichexpression includes the pharmaceutically acceptable salts, and thesolvates, e.g., hydrates, where the context so permits. Similarly,reference to intermediates, whether or not they themselves are claimed,is meant to embrace their salts, and solvates, where the context sopermits.

As used herein, the term “isotopic variant” refers to a compound thatcontains unnatural proportions of isotopes at one or more of the atomsthat constitute such compound. For example, an “isotopic variant” of acompound can be radiolabeled, that is, contain one or morenon-radioactive isotopes, such as for example, deuterium (²H or D),carbon-13 (¹³C), nitrogen-15 (¹⁵N), or the like. It will be understoodthat, in a compound where such isotopic substitution is made, thefollowing atoms, where present, may vary, so that for example, anyhydrogen may be ²H/D, any carbon may be ¹³C, or any nitrogen may be ¹⁵N,and that the presence and placement of such atoms may be determinedwithin the skill of the art. Likewise, the invention may include thepreparation of isotopic variants with radioisotopes, in the instance forexample, where the resulting compounds may be used for drug and/orsubstrate tissue distribution studies. Radiolabeled compounds of theinvention can be used in diagnostic methods such as Single-photonemission computed tomography (SPECT). The radioactive isotopes tritium,i.e. ³H, and carbon-14, i.e. ¹⁴C, are particularly useful for their easeof incorporation and ready means of detection. Further, compounds may beprepared that are substituted with positron emitting isotopes, such as¹¹C, ¹⁸F, ¹⁵O and ¹³N, and would be useful in Positron EmissionTopography (PET) studies for examining substrate receptor occupancy.

All isotopic variants of the compounds of the invention, radioactive ornot, are intended to be encompassed within the scope of the invention.

It is also to be understood that compounds that have the same molecularformula but differ in the nature or sequence of bonding of their atomsor the arrangement of their atoms in space are termed “isomers.” Isomersthat differ in the arrangement of their atoms in space are termed“stereoisomers.”

Stereoisomers that are not mirror images of one another are termed“diastereomers” and those that are non-superimposable mirror images ofeach other are termed “enantiomers.” When a compound has an asymmetriccenter, for example, it is bonded to four different groups, a pair ofenantiomers is possible. An enantiomer can be characterized by theabsolute configuration of its asymmetric center and is described by theR- and S-sequencing rules of Cahn and Prelog, or by the manner in whichthe molecule rotates the plane of polarized light and designated asdextrorotatory or levorotatory (i.e., as (+) or (−)-isomersrespectively). A chiral compound can exist as either individualenantiomer or as a mixture thereof. A mixture containing equalproportions of the enantiomers is called a “racemic mixture.”

“Tautomers” refer to compounds that are interchangeable forms of aparticular compound structure, and that vary in the displacement ofhydrogen atoms and electrons. Thus, two structures may be in equilibriumthrough the movement of π electrons and an atom (usually H). Forexample, enols and ketones are tautomers because they are rapidlyinterconverted by treatment with either acid or base. Another example oftautomerism is the aci- and nitro-forms of phenyl nitromethane, that arelikewise formed by treatment with acid or base.

Tautomeric forms may be relevant to the attainment of the optimalchemical reactivity and biological activity of a compound of interest.

The compounds of this invention may possess one or more asymmetriccenters; such compounds can therefore be produced as individual (R)- or(S)-stereoisomers or as mixtures thereof.

Unless indicated otherwise, the description or naming of a particularcompound in the specification and claims is intended to include bothindividual enantiomers and mixtures, racemic or otherwise, thereof. Themethods for the determination of stereochemistry and the separation ofstereoisomers are well-known in the art.

The present invention is directed to compounds of Formula I:

-   -   wherein    -   X is CR₁ or N;    -   Y is CR₂ or N;    -   Z is NH, N—CH₃, or O;    -   R₁ is H, alkoxy, triazolyl, oxazolyl, isoxazolyl, oxadiazolyl,        or pyrimidinyl, wherein triazolyl, oxazolyl, isoxazolyl,        oxadiazolyl, or pyrimidinyl is optionally substituted with up to        two substituents selected from halo and alkyl;    -   R₂ is H, alkyl, or halo;    -   R₃ is H, alkyl, alkoxy, halo, triazolyl, oxazolyl, or        pyrimidinyl, wherein triazolyl, oxazolyl, or pyrimidinyl is        optionally substituted with up to two substituents selected from        halo and alkyl;    -   R₄ is alkyl;    -   R₅ is pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;        quinoxalinyl; pyrazinyl; thiadiazolyl; or quinazolinyl;        -   wherein the pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;            quinoxalinyl; pyrazinyl; or quinazolinyl is optionally            substituted with one or two substituents independently            selected from the group consisting of alkyl, halo, or            phenyl; and    -   R₆ is H or alkyl.

In one aspect, the invention is directed to compounds of Formula I:

-   -   wherein    -   X is CR₁ or N;    -   Y is CR₂ or N;    -   Z is NH or 0;    -   R₁ is alkoxy, triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or        pyrimidinyl;    -   R₂ is H, alkyl, or halo;    -   R₃ is H, alkyl, alkoxy, halo, triazolyl, oxazolyl, or        pyrimidinyl;    -   R₄ is alkyl;    -   R₅ is pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;        quinoxalinyl; pyrazinyl; or quinazolinyl;        -   wherein the pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;            quinoxalinyl; pyrazinyl; or quinazolinyl is optionally            substituted with one or two substituents independently            selected from the group consisting of alkyl, halo, or            phenyl; and    -   R₆ is H or alkyl.

Enantiomers and diastereomers of the compounds of Formula I are alsowithin the scope of the invention. Also within the scope of theinvention are the pharmaceutically acceptable salts of the compounds ofFormula I, as well as the pharmaceutically acceptable salts of theenantiomers and diastereomers of the compounds of Formula I. Also withinthe scope of the invention are isotopic variants of compounds of FormulaI, such as, for example, deuterated compounds of Formula I.

In preferred embodiments of the invention, Z is NH. In other preferredembodiments of the invention, Z is O. In some embodiments, Z is N—CH₃.

Preferably, in the compounds of the invention, R₄ is an alkyl groupcontaining from 1 to 6 carbon atoms (C₁₋₆alkyl). Examples of preferredalkyl groups for R₄ include methyl, ethyl, and propyl. In exemplaryembodiments of the invention, R₄ is methyl.

In some embodiments of the invention, X is CR₁ and Y is CR₂.

In other embodiments of the invention, X is N and Y is CR₂.

In still other embodiments, X is CR₁ and Y is N.

In yet other embodiments, X and Y are each N.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is alkoxy, preferably methoxy, ethoxy, or propoxy.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is triazolyl, with 1,2,3-triazolyl being preferred. In preferredembodiments, the 1,2,3-triazolyl is attached through the 2-positionnitrogen atom. In other embodiments, the 1,2,3-triazolyl is attachedthrough the 1-position nitrogen atom. In some embodiments, R₁ istriazolyl optionally substituted with up to two substituents selectedfrom halo and alkyl.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is oxazolyl, which can be attached through any available atom,preferably attached through the 2-position carbon. In some embodiments,R₁ is oxazolyl optionally substituted with up to two substituentsselected from halo and alkyl.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is isoxazolyl, which can be attached through any available atom. Insome embodiments, R₁ is isoxazolyl optionally substituted with up to twosubstituents selected from halo and alkyl.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is oxadiazolyl, preferably 1,2,4-oxadiazolyl attached through the5-position carbon, although any oxadiazolyl, attached through anyavailable carbon, is within the scope of the invention. In someembodiments, R₁ is oxadiazolyl optionally substituted with up to twosubstituents selected from halo and alkyl. The oxadiazolyl group canoptionally be substituted with C₁₋₆alkyl, for example methyl. Inexemplary embodiments, the substituted oxadiazolyl moiety is1,2,4-oxadiazolyl substituted with methyl.

In those embodiments of the invention wherein X is CR₁, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is CR₁ and Y is N,R₁ is pyrimidinyl, which can be attached through any available atom. Insome embodiments, R₁ is pyrimidinyl optionally substituted with up totwo substituents selected from halo and alkyl. The pyrimidinyl can beoptionally substituted with, for example, halogen such as fluoro.Preferably, the pyrimidinyl moiety is attached through the 2-positioncarbon.

In preferred embodiments of the invention wherein Y is CR₂, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is N and Y is CR₂,R₂ is H.

In some embodiments of the invention wherein Y is CR₂, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is N and Y is CR₂,R₂ is halo, preferably F, Cl, or Br, with F being more preferred.

In those embodiments of the invention wherein Y is CR₂, for example,those embodiments wherein X is CR₁ and Y is CR₂ or X is N and Y is CR₂,R₂ is alkyl. Preferably, the alkyl group has from one to six carbonatoms (C₁₋₆alkyl). In exemplary embodiments, R₂ is methyl.

In some embodiments of the invention, R₃ is H. In other embodiments, R₃is alkyl, preferably methyl. In some embodiments, the alkyl group can besubstituted with, for example, one or more halogen atoms. One exemplarysubstituted alkyl group is trifluoromethyl.

In yet other embodiments, R₃ is alkoxy, preferably methoxy or ethoxy. Instill other embodiments, R₃ is halo, preferably F, Br, or Cl, with F andBr being more preferred.

In other exemplary embodiments of the invention, R₃ is triazolyl, with1,2,3-triazolyl being preferred. In some embodiments, the1,2,3-triazolyl is attached through the 2-position nitrogen atom. Inother embodiments, the 1,2,3-triazolyl is attached through the1-position nitrogen atom.

In still other embodiments of the invention, R₃ is oxazolyl, which canbe attached through any available atom, preferably attached through the2-position carbon.

In yet other embodiments, R₃ is pyrimidinyl, which can be attachedthrough any available atom. The pyrimidinyl can be optionallysubstituted with, for example, halogen such as fluoro. Preferably, thepyrimidinyl moiety is attached through the 2-position carbon.

In some embodiments of the invention, R₅ is pyridyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The pyridyl can be attachedthrough any one of the 2-, 3-, or 4-position carbon atoms. In thoseembodiments where the pyridyl is substituted, the substitutents areindependently selected from alkyl, for example methyl, substitutedalkyl, for example, trihaloalkyl such as trifluoromethyl, and halo suchas F, Br, and Cl.

In some embodiments of the invention, R₅ is benzoxazolyl, which can beattached through any available atom, and which can be optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. In those embodiments wherethe benzoxazolyl is substituted, the substitutents are independentlyselected from alkyl, for example methyl, substituted alkyl, for example,trihaloalkyl such as trifluoromethyl, and halo such as F, Br, and Cl.

In some embodiments of the invention, R₅ is pyrimidinyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The pyrimidinyl can beattached through any one of the 2-, 4-, or 5-position carbon atoms. Inthose embodiments where the pyrimidinyl is substituted, thesubstitutents are independently selected from alkyl, for example methyl,substituted alkyl, for example trihaloalkyl such as trifluoromethyl, andhalo such as F, Br, and Cl.

In some embodiments of the invention, R₅ is pyridazinyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The pyridazinyl can beattached through any one of the 3- or 4-position carbon atoms. In thoseembodiments where the pyridazinyl is substituted, the substitutents areindependently selected from alkyl, for example methyl, substitutedalkyl, for example trihaloalkyl such as trifluoromethyl, and halo suchas F, Br, and Cl.

In some embodiments of the invention, R₅ is quinoxalinyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The quinoxalinyl can beattached through any one of the 2-, 3-, 5-, or 6-position carbon atoms.In those embodiments where the quinoxalinyl is substituted, thesubstitutents are independently selected from alkyl, for example methyl,substituted alkyl, for example trihaloalkyl such as trifluoromethyl, andhalo such as F, Br, and Cl.

In some embodiments of the invention, R₅ is pyrazinyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The pyrazinyl can beattached through any one of the 2- or 3-position carbon atoms. In thoseembodiments where the pyrazinyl is substituted, the substitutents areindependently selected from alkyl, for example methyl, substitutedalkyl, for example trihaloalkyl such as trifluoromethyl, and halo suchas F, Br, and Cl.

In some embodiments of the invention, R₅ is quinazolinyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, or phenyl. The quinazolinyl can beattached through any one of the 2-, 4-, 5-, 6-, 7-, or 8-position carbonatoms. In those embodiments where the quinazolinyl is substituted, thesubstitutents are independently selected from alkyl, for example methyl,substituted alkyl, for example trihaloalkyl such as trifluoromethyl, andhalo such as F, Br, and Cl.

In preferred embodiments of the invention, R₆ is H. In other embodimentsof the invention, R₆ is alkyl, preferably methyl.

In some embodiments of Formula I, R₁ is H and R₃ is as defined above forFormula I, preferably R₃ is triazolyl, oxazolyl, pyridyl or pyrimidinyl.In other embodiments of Formula I, R₃ is H and R₁ is as defined abovefor Formula I, preferably R₁ is triazolyl, oxazolyl, isoxazolyl,oxadiazolyl, pyridyl, or pyrimidinyl.

In some embodiments of Formula I, the group

is a pyridyl group, preferably X is N, R₃ is a ring selected fromtriazolyl, oxazolyl, or pyrimidinyl; preferably triazolyl, preferably atthe ortho position; R₆ is H or alkyl, preferably methyl; Z is NH or O,preferably O; R₅ is a heteroaryl, preferably pyridyl or pyrazinyl. Insome of such embodiments, R₃ is a ring at the ortho position relative tothe carbonyl group in Formula I, and R₆ is at the ortho, meta or paraposition on the relative to the carbonyl group in Formula I, preferablyR₆ is at the meta position adjacent to R₃. In some other suchembodiments, R₃ is a ring at the ortho position relative to the carbonylgroup in Formula I, and R₆ is at the ortho, meta or para positionrelative to the carbonyl group in Formula I, preferably R₆ is at themeta position not adjacent to R₃. R₃ and R₅ may be optionallysubstituted as described above.In some embodiments of Formula I, the group

is a pyridyl group, preferably Y is N, R₃ is H, R₁ is a ring selectedfrom triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl;preferably triazolyl; R₆ is H or alkyl, preferably methyl; Z is NH or O,preferably O; R₅ is a heteroaryl, preferably pyridyl or pyrazinyl. Insome of such embodiments, R₃ is a ring at the ortho position relative tothe carbonyl group in Formula I, and R₆ is at the ortho, meta or paraposition on the relative to the carbonyl group in Formula I, preferablyR₆ is at the meta position adjacent to R₁. In some other suchembodiments, R₁ is a ring at the ortho position relative to the carbonylgroup in Formula I, and R₆ is at the ortho, meta or para positionrelative to the carbonyl group in Formula I, preferably R₆ is at themeta position not adjacent to R₁. R₁, R₃ and R₅ may be optionallysubstituted as described above

In some embodiments of Formula I, the group

is a phenyl group, R₁ is H, R₃ is a ring selected from triazolyl,oxazolyl, or pyrimidinyl; preferably triazolyl; R₆ is H or alkyl,preferably methyl; Z is NH or O, preferably O; R₅ is a heteroaryl,preferably pyridyl or pyrazinyl. In some of such embodiments, R₃ is aring at the ortho position relative to the carbonyl group in Formula I,and R₆ is at the ortho, meta or para position on the relative to thecarbonyl group in Formula I, preferably R₆ is at the meta positionadjacent to R₃. In some other such embodiments, R₃ is a ring at theortho position relative to the carbonyl group in Formula I, and R₆ is atthe ortho, meta or para position relative to the carbonyl group inFormula I, preferably R₆ is at the meta position not adjacent to R₃. R₃and R₅ may be optionally substituted as described above.

In another aspect provided herein is a compound of Formula IA:

-   -   wherein    -   ring A is a heteroaryl ring;    -   Z is NH, N—CH₃, or O;    -   R₁ is H, alkoxy, phenyl, triazolyl, oxazolyl, isoxazolyl,        oxadiazolyl, or pyrimidinyl, wherein triazolyl, oxazolyl,        isoxazolyl, oxadiazolyl, or pyrimidinyl is optionally        substituted with up to two substituents selected from halo and        alkyl;    -   R₂ is H, alkyl, or halo;    -   R₃ is H, alkyl, alkoxy, halo, triazolyl, oxazolyl, or        pyrimidinyl, wherein triazolyl, oxazolyl, or pyrimidinyl is        optionally substituted with up to two substituents selected from        halo and alkyl;    -   R₄ is alkyl;    -   R₅ is pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;        quinoxalinyl; pyrazinyl; thiadiazolyl; or quinazolinyl;        -   wherein the pyridyl; benzoxazolyl; pyrimidinyl; pyridazinyl;            quinoxalinyl; pyrazinyl; or quinazolinyl is optionally            substituted with one or two substituents independently            selected from the group consisting of alkyl, halo, or            phenyl; and    -   R₆ is H or alkyl.

In some embodiments of Formula IA, ring A is a 5-membered heteroarylring. In other embodiments of Formula IA, ring A is a pyrazinyl ring.All the embodiments described above for Formula I with respect to thevariables R₁, R₂, R₃, R₄, R₅, R₆, and Z are also applicable to compoundsof Formula IA and are expressly contemplated herein.

The invention relates to methods of using the compounds described hereinto treat subjects diagnosed with or suffering from a disease, disorder,or condition mediated by orexin receptor activity. These methods areaccomplished by administering to the subject a compound of theinvention. In some embodiments, the compounds described herein areselective for orexin-1 receptor activity. In some embodiments, thecompounds described herein are selective for orexin-1 receptor activityover orexin-2 receptor activity.

Diseases, disorders, and conditions mediated by orexin receptor activityinclude disorders of the sleep-wake cycle, insomnia, restless legssyndrome, jet-lag, disturbed sleep, sleep disorders secondary toneurological disorders, mania, depression, manic depression,schizophrenia, pain syndromes, fibromyalgia, neuropathic pain,catatonia, Parkinson's disease, Tourette's syndrome, anxiety, delirium,dementia, overweight, obesity, or conditions related to overweight orobesity, insulin resistance, type II diabetes, hyperlipidemia,gallstones, angina, hypertension, breathlessness, tachycardia,infertility, sleep apnea, back and joint pain, varicose veins,osteoarthritis, hypertension, tachycardia, arrhythmias, angina pectoris,acute heart failure, ulcers, irritable bowel syndrome, diarrheagastroesophageal reflux, mood disorders, post-traumatic stress disorder,panic disorders, attention deficit disorders, cognitive deficiencies, orsubstance abuse.

In one aspect, compounds of the invention are particularly suited forthe treatment of mood disorders. Non-limiting examples of mood disordersinclude anxiety-related mood disorders, depression, panic-related mooddisorders, stress related mood disorders and the like. In anotheraspect, compounds of the invention are suitable for the treatment ofpost-traumatic stress disorder, panic disorders, attention deficitdisorders, cognitive deficiencies, or substance abuse (e.g., morphineabuse, cocaine abuse, alcohol abuse and the like). It will be understoodthat certain disorders such as, for example, depression and/orschizophrenia and/or substance abuse and/or cognitive impairments alsohave elements of anxiety and/or panic and/or stress associated with themand the treatment of such conditions and/or combinations of conditionsare also contemplated within the scope of embodiments presented herein.In some embodiments, advantageously, compounds of the invention treat amood disorder (e.g., anxiety) with reduced concomitant sedation and/orwith reduced effect on sleep (e.g. attenuated arousal effects). In oneembodiment, compounds of the invention are particularly suited for thetreatment of anxious depression. In another embodiment, compounds of theinvention are particularly suited for the treatment of panic,schizophrenia, and substance abuse.

Sleep disorders include, but are not limited to, sleep-wake transitiondisorders, insomnia, restless legs syndrome, jet-lag, disturbed sleep,and sleep disorders secondary to neurological disorders (e.g., manias,depressions, manic depression, schizophrenia, and pain syndromes (e.g.,fibromyalgia, neuropathic).

Metabolic disorders include, but are not limited to, overweight orobesity and conditions related to overweight or obesity, such as insulinresistance, type II diabetes, hyperlipidemia, gallstones, angina,hypertension, breathlessness, tachycardia, infertility, sleep apnea,back and joint pain, varicose veins and osteoarthritis.

Neurological disorders include, but are not limited to, Parkinson'sdisease, Alzheimer's disease, Tourette's Syndrome, catatonia, anxiety,delirium and dementias.

In treatment methods according to the invention, a therapeuticallyeffective amount of a pharmaceutical agent according to the invention isadministered to a subject suffering from or diagnosed as having such adisease, disorder, or condition. A “therapeutically effective amount”means an amount or dose sufficient to generally bring about the desiredtherapeutic or prophylactic benefit in patients in need of suchtreatment for the designated disease, disorder, or condition. Effectiveamounts or doses of the compounds of the present invention may beascertained by routine methods such as modeling, dose escalation studiesor clinical trials, and by taking into consideration routine factors,e.g., the mode or route of administration or drug delivery, thepharmacokinetics of the compound, the severity and course of thedisease, disorder, or condition, the subject's previous or ongoingtherapy, the subject's health status and response to drugs, and thejudgment of the treating physician. An example of a dose is in the rangeof from about 0.001 to about 200 mg of compound per kg of subject's bodyweight per day, preferably about 0.05 to 100 mg/kg/day, or about 1 to 35mg/kg/day, in single or divided dosage units (e.g., BID, TID, QID). Fora 70-kg human, an illustrative range for a suitable dosage amount isfrom about 0.05 to about 7 g/day, or about 0.2 to about 2.5 g/day.

Once improvement of the patient's disease, disorder, or condition hasoccurred, the dose may be adjusted for preventative or maintenancetreatment. For example, the dosage or the frequency of administration,or both, may be reduced as a function of the symptoms, to a level atwhich the desired therapeutic or prophylactic effect is maintained. Ofcourse, if symptoms have been alleviated to an appropriate level,treatment may cease. Patients may, however, require intermittenttreatment on a long-term basis upon any recurrence of symptoms.

In addition, the compounds of the invention may be used in combinationwith additional active ingredients in the treatment of the aboveconditions. The additional active ingredients may be coadministeredseparately with a compound of the invention or included with such anagent in a pharmaceutical composition according to the invention. In anexemplary embodiment, additional active ingredients are those that areknown or discovered to be effective in the treatment of conditions,disorders, or diseases mediated by orexin activity, such as anotherorexin modulator or a compound active against another target associatedwith the particular condition, disorder, or disease. The combination mayserve to increase efficacy (e.g., by including in the combination acompound potentiating the potency or effectiveness of an active agentaccording to the invention), decrease one or more side effects, ordecrease the required dose of the active agent according to theinvention.

The compounds of the invention are used, alone or in combination withone or more additional active ingredients, to formulate pharmaceuticalcompositions of the invention. A pharmaceutical composition of theinvention comprises: (a) an effective amount of at least one compound inaccordance with the invention; and (b) a pharmaceutically acceptableexcipient.

Delivery forms of the pharmaceutical compositions containing one or moredosage units of the active agents may be prepared using suitablepharmaceutical excipients and compounding techniques known or thatbecome available to those skilled in the art. The compositions may beadministered in the inventive methods by a suitable route of delivery,e.g., oral, parenteral, rectal, topical, or ocular routes, or byinhalation.

The preparation may be in the form of tablets, capsules, sachets,dragees, powders, granules, lozenges, powders for reconstitution, liquidpreparations, or suppositories. Preferably, the compositions areformulated for intravenous infusion, topical administration, or oraladministration.

For oral administration, the compounds of the invention can be providedin the form of tablets or capsules, or as a solution, emulsion, orsuspension. To prepare the oral compositions, the compounds may beformulated to yield a dosage of, e.g., from about 0.05 to about 100mg/kg daily, or from about 0.05 to about 35 mg/kg daily, or from about0.1 to about 10 mg/kg daily. For example, a total daily dosage of about5 mg to 5 g daily may be accomplished by dosing once, twice, three, orfour times per day.

Oral tablets may include a compound according to the invention mixedwith pharmaceutically acceptable excipients such as inert diluents,disintegrating agents, binding agents, lubricating agents, sweeteningagents, flavoring agents, coloring agents and preservative agents.Suitable inert fillers include sodium and calcium carbonate, sodium andcalcium phosphate, lactose, starch, sugar, glucose, methyl cellulose,magnesium stearate, mannitol, sorbitol, and the like. Exemplary liquidoral excipients include ethanol, glycerol, water, and the like. Starch,polyvinyl-pyrrolidone (PVP), sodium starch glycolate, microcrystallinecellulose, and alginic acid are suitable disintegrating agents. Bindingagents may include starch and gelatin. The lubricating agent, ifpresent, may be magnesium stearate, stearic acid or talc. If desired,the tablets may be coated with a material such as glyceryl monostearateor glyceryl distearate to delay absorption in the gastrointestinaltract, or may be coated with an enteric coating.

Capsules for oral administration include hard and soft gelatin capsules.To prepare hard gelatin capsules, compounds of the invention may bemixed with a solid, semi-solid, or liquid diluent. Soft gelatin capsulesmay be prepared by mixing the compound of the invention with water, anoil such as peanut oil or olive oil, liquid paraffin, a mixture of monoand di-glycerides of short chain fatty acids, polyethylene glycol 400,or propylene glycol.

Liquids for oral administration may be in the form of suspensions,solutions, emulsions or syrups or may be lyophilized or presented as adry product for reconstitution with water or other suitable vehiclebefore use. Such liquid compositions may optionally contain:pharmaceutically-acceptable excipients such as suspending agents (forexample, sorbitol, methyl cellulose, sodium alginate, gelatin,hydroxyethylcellulose, carboxymethylcellulose, aluminum stearate gel andthe like); non-aqueous vehicles, e.g., oil (for example, almond oil orfractionated coconut oil), propylene glycol, ethyl alcohol, or water;preservatives (for example, methyl or propyl p-hydroxybenzoate or sorbicacid); wetting agents such as lecithin; and, if desired, flavoring orcoloring agents.

The active agents of this invention may also be administered by non-oralroutes. For example, the compositions may be formulated for rectaladministration as a suppository. For parenteral use, includingintravenous, intramuscular, intraperitoneal, or subcutaneous routes, thecompounds of the invention may be provided in sterile aqueous solutionsor suspensions, buffered to an appropriate pH and isotonicity or inparenterally acceptable oil. Suitable aqueous vehicles include Ringer'ssolution and isotonic sodium chloride. Such forms will be presented inunit-dose form such as ampules or disposable injection devices, inmulti-dose forms such as vials from which the appropriate dose may bewithdrawn, or in a solid form or pre-concentrate that can be used toprepare an injectable formulation. Illustrative infusion doses may rangefrom about 1 to 1000 .mu.g/kg/minute of compound, admixed with apharmaceutical carrier over a period ranging from several minutes toseveral days.

For topical administration, the compounds may be mixed with apharmaceutical carrier at a concentration of about 0.1% to about 10% ofdrug to vehicle. Another mode of administering the compounds of theinvention may utilize a patch formulation to affect transdermaldelivery.

Compounds of the invention may alternatively be administered in methodsof this invention by inhalation, via the nasal or oral routes, e.g., ina spray formulation also containing a suitable carrier.

Exemplary compounds useful in methods of the invention will now bedescribed by reference to the illustrative synthetic schemes for theirgeneral preparation below and the specific examples that follow.Artisans will recognize that, to obtain the various compounds herein,starting materials may be suitably selected so that the ultimatelydesired substituents will be carried through the reaction scheme with orwithout protection as appropriate to yield the desired product.Alternatively, it may be necessary or desirable to employ, in the placeof the ultimately desired substituent, a suitable group that may becarried through the reaction scheme and replaced as appropriate with thedesired substituent. Unless otherwise specified, the variables are asdefined above in reference to Formula (I). Reactions may be performedbetween the melting point and the reflux temperature of the solvent, andpreferably between 0° C. and the reflux temperature of the solvent.Reactions may be heated employing conventional heating or microwaveheating. Reactions may also be conducted in sealed pressure vesselsabove the normal reflux temperature of the solvent.

Schemes 1-6 below and the Examples section below (Intermediates A1-A-39)provide methods for the synthesis of certain exemplary intermediateshaving the structure

where LG is Cl, OH or OC(O)Alk.

Intermediate compounds of formulas (IIIa) and (IIIb) are prepared, asoutlined in Scheme 1, from commercially available or syntheticallyaccessible compounds of formula (A). X and Y are as defined in Formula Iabove. Compounds of formulas (IIa) and (IIb), are obtained by reacting acompound of formula (A), where R_(3A) is —H, -alkyl, or -alkoxy, withcommercially available 1,2,3-triazole, in the presence of, for example,K₂CO₃ in DMF or dioxane, at temperatures ranging from about 60° C. toabout 100° C. Compounds of formulas (IIIa) and (IIIb) are obtained byreacting compounds of formula (II) in the presence of a base such asNaOH in a solvent such as EtOH at temperatures ranging from about 80° C.to about 100° C. One skilled in the art will recognize that1,2,3-triazole can exist in two tautomeric forms defined as2H-[1,2,3]triazole and 1H-[1,2,3]triazole thus accounting for theformation of (IIIa) and (IIIb).

Intermediate compounds of formula (III) can be prepared, as outlined inScheme 2, from commercially available or synthetically accessiblecompounds of formula (IV_(a-c)). Compounds of formulas (Va), (Vb) and(III) are obtained by reacting compounds of formula (IVa), (IVb) and(IVc) where Hal is —Br, or —I; W is CO₂H, CO₂Alkyl, or CN and R_(3A) andR_(4A) are —H, halo, —C₁₋₄alkyl, —C₁₋₄alkoxy or R_(3A) and R_(4A),together with the atoms to which they are attached, form a 6-memberedaryl or 6-membered heteroaryl ring, with commercially available1,2,3-triazole, in the presence of, for example, copper(I)iodide, Cs₂CO₃and trans-N,N′-dimethyl-1,2-cyclohexanediamine in for example, DMF or1,4-dioxane, at temperatures ranging from about 60° C. to about 120° C.Compounds of formula (IVc) can be converted to the corresponding esters(Vb) by treatment with, for example, alkyl iodide in the presence of abase such as K₂CO₃ in a solvent such as DMF. Compounds of formula (III)are also obtained by reacting compounds of formula (Va) and (Vb) in thepresence of a base such as NaOH in a solvent such as EtOH attemperatures ranging from about 80° C. to about 100° C. It will beunderstood that the heterocycle in (Va) and (Vb) is not limited totriazole and may be any other suitable heterocycle.

Intermediate compounds of formula (IX) are prepared, as outlined inScheme 3, from commercially available or synthetically accessiblecompounds of formula (VI) where X is CR₁ or N, Y is CR₂ or N, R₁ and R₂are H, alkyl, halo or alkoxy, R_(3A) is —H, halo, -alkyl, -alkoxy and R₆is H or alkyl, G is SnBu₃ or 4,4,5,5 tetramethyl-1, dioxaboralane andHal is Cl, or Br, preferably Br in this case. Compounds of formula(VIII) are obtained by reacting a compound of formula (VI) withcommercially available (VII) in the presence of a catalyst such as1,1′-Bis(di-tert-butylphosphino)ferrocene palladium dichloride and abase such as Na₂CO₃ in a solvent such as 2-MeTHF or THF at temperaturesranging from about 60° C. to about 90° C. Compounds of formula (IX) areobtained by reacting a compound of formula (VIII) in the presence of abase such as NaOH in a solvent such as MeOH at temperatures ranging fromabout 80° C. to about 100° C. or acids such as H₂SO₄ in solvents such asH₂O at temperatures ranging from 80° C. to 100° C. It will be understoodthat the heterocycle in (VII) is not limited to pyrimidine and may beany other suitable heterocycle.

Intermediate compound (XIV) is prepared, as outlined in Scheme 4, fromcommercially available compound (X). Compound (XI) is obtained byreacting compound (X) with commercially available acrolein in a solventsuch as 1,4-dioxane at temperatures of about 200° C. in, for example, amicrowave reactor. Compound (XII) can be prepared from compound (XI) bytreatment with an acid such as HBr in a solvent such as toluene at atemperature of about 90° C. Compound (XIII) can be obtained by treatmentof compound (XII) with, for example, commercially available iodoethaneand a base such as K₂CO₃ in a solvent such as DMF at temperaturesranging from about 45° C. to about 65° C. Compound (XIV) is obtained bytreating compound (XIII) with a base such as NaOH in a solvent such asMeOH at temperatures ranging from about 80° C. to about 100° C.

Intermediate compounds of formula (XVI) are prepared, as outlined inScheme 5, from a commercially available or synthetically accessiblecompounds of formula (XIV) where R₃ is —H, -alkyl, or -alkoxy and Hal isCl or Br. Compounds of formula (XV) are obtained by reacting a compoundof formula (XIV) with commercially available (VII) in the presence of acatalyst such as Pd(dppf)Cl₂ and a base such as Na₂CO₃ in a solvent suchas 2-MeTHF at temperatures ranging from about 75° C. to about 150° C.Compounds of formula (XVI) are obtained by reacting a compound offormula (XV) in the presence of a base such as NaOH in a solvent such asMeOH at temperatures ranging from about 80° C. to about 100° C.

Intermediate compounds of formula (XXI) can be prepared, as outlined inScheme 6, from a commercially available or synthetically accessiblecompounds of formula (XVII) where Hal is Br or I; and where R_(3A) is—H, halo, -alkyl, -alkoxy and R₆ is H or alkyl. Compounds of formula(XVIIIa) can be converted to the corresponding ester (XVIIIb) bytreatment with, for example, thionyl chloride in a solvent such as MeOH.Compounds of the formula (XX) are obtained by reacting compounds offormula (XVIIIb) with commercially available compounds of the formulaXIX where L is a heterocyle such as pyrazole, pyridyl, or oxazole; G isSnBu₃ or 4,4,5,5 tetramethyl-1,dioxaboralane and R_(1A) and R_(2A) are—H, -alkyl, or -alkoxy; or R_(1A) and R_(2A) are —H, halo, -alkyl, or-alkoxy; in the presence of a catalyst such as Pd(Ph₃P)₄ and a base suchas Na₂CO₃ in a mixture of solvents such as DME and H₂O at temperaturesranging from about 100° C. to about 150° C. Compounds of formula (XXI)are obtained by reacting a compound of formula (XX) in the presence of abase such as NaOH in a solvent such as MeOH at temperatures ranging fromabout 80° C. to about 100° C.

Referring to Scheme 7, compounds of formula (+)-(XXXVIa) and(−)-(XXXVIb) were synthesized from compounds of formula (XXII) where PG₁is, for example, a Boc protecting group, R₄ is alkyl and Bn represents abenzyl group, —CH₂Ph. Treatment of compounds of formula (XXII) withhydrogen in the presence of, for example, a metal catalyst such as PtO₂,Pd/C, or Pd(OH)₂ in solvents such as AcOH provide compounds of formula(±)-(XXIII) as a mixture of diastereomers favoring the cis diastereomerof formula (±)-(XXIII). Compounds of formula (±)-(XXXIV) weresynthesized from compounds of formula (±)-(XXIII) upon treatment with abase such as sodium methoxide in solvents such as methanol at refluxtemperature. The amine in these compounds was protected as PG1 usingreagents such as Boc₂O in the presence of bases such as Na₂CO₃ insolvents such as THF and water. Compounds of formula (±)-(XXXV) weresynthesized from compounds of formula (±)-(XXXIV) by treatment withbases such as sodium hydroxide, lithum hydroxide or potassium hydroxidein solvents such as water, THF or methanol. Compounds of formula(±)-(XXXVI) were synthesized from compounds of formula (±)-(XXXV) bytreatment with, for example, DPPA in solvents such as toluene in thepresence of bases such as TEA at temperatures ranging from about 70-110°C. followed by, for example, benzyl alcohol followed by continuedheating to temperatures ranging from about 70-110° C. Compounds offormula (±)-(XXXVI) were resolved into individual enantiomers using SFCchromatography on a chiral SFC (CHIRALPAK AD-H 5 mM 250×30 mm) column toprovide compounds of formula (+)-(XXXVIa) and (−)-(XXXVIb).

Referring to Scheme 8, compounds of formula (±)-(XXXVIII) weresynthesized from compounds of formula (±)-(XXIII) where PG₁ is, forexample, a Boc protecting group, R₄ is alkyl and Bn represents a benzylgroup, —CH₂Ph. The amine in compounds of formula (±)-(XXIII) wasprotected as PG₁ using reagents such as Boc₂O in the presence of basessuch as Na₂CO₃ in solvents such as THF and water. Compounds of formula(±)-(XXXVII) were synthesized from compounds of formula (±)-(XXIII) bytreatment with bases such as sodium hydroxide, lithum hydroxide, orpotassium hydroxide in solvents such as water, THF, or methanol afterprotection with PG₁ as described. Compounds of formula (±)-(XXXVIII)were synthesized from compounds of formula (±)-(XXXVII) by treatmentwith, for example, DPPA in solvents such as toluene in the presence ofbases such as TEA at temperatures ranging from about 70-110° C. followedby benzyl alcohol followed by continued heating to temperatures rangingfrom about 70-110° C.

Referring to Scheme 9, compounds of formula XLII were synthesized fromcompounds of formula (±)-(XXXVIa) where PG₁ is, for example, a Bocprotecting group, R₄ is alkyl, LG is Cl, OH or OC(O)Alk, W is Cl, Br, For I and Bn represents a benzyl group, —CH₂Ph. Compounds of formula(XXXIX) were obtained from compounds of formula (±)-(XXXVIa) bytreatment with, for example, Pd catalysts such as 10 wt % Pd/C wetDegussa under an atmosphere of H₂ in a solvent such as EtOH to givecompound of formula (XXXIX). Compounds of formula (XL) were obtainedfrom compounds of formula (XXXIX) using compounds R₅—W in a suitablesolvent such as DMSO or DMF in the presence of a base such as K₂CO₃ at atemperature of about 70° C. Compounds of formula (XL) were also obtainedwhen compounds of formula (XXXIX) and R₅—W were treated with, forexample, Pd catalysts such as Pd(OAc)₂ or Pd(dba)₂, ligands such asracemic BINAP or Q-PHOS, a base such as sodium tert-butoxide in asolvent such as toluene at a temperature of about 70° C. Compounds offormula (XLI) were obtained from compounds of formula (XL) when treatedwith an acid such as HCl or TFA in a suitable solvent such as EtOAc orDCM at room temperature. Compounds of formula (XLII) were obtained fromcompounds of formula (XLI) using compounds of formula (XLIII) in asuitable solvent such as DMF or DCM in the presence of a peptidecoupling reagent such as HATU, HBTU or T3P, a base such as DIPEA, asolvent such as DMF, ACN, THF or DCM at a temperature ranging from rt toabout 45° C. One skilled in the art will recognize similar chemistry canbe done with compounds of formula (±)-XXXVI to give compounds of formula(±)-XLII.

Referring to Scheme 10 compounds of formula (±)-XLIV were synthesizedfrom compounds of formula (±)-XXXVIII using similar chemistry asdescribed for the compounds in Scheme 9.

Referring to Scheme 11 compounds of formula (±)-XLV were synthesizedfrom compounds of formula (±)-XLVI (J. Org. Chem. 2008, 73, 2898) usingsimilar chemistry as described for the compounds in Scheme 9.

In one group of embodiments, provided herein is a compound of Formula Iof Examples 1-172, 174-177, 179-273 with structures and names as setforth in the Examples section below. In another group of embodiments,provided herein is a compound of Formula I of Examples 1-146 withstructures and names as set forth in the Examples section below. In yetanother embodiment, provided herein is a compound of Formula I ofExamples 147-172, 174-177, 179-273 with structures and names as setforth in the Examples section below. In one group of embodiments,provided herein is a compound of Formula I or Formula IA havingstructures and names as set forth in Table 2 below. In a further groupof embodiments, provided herein is a compound of Formula IA of Examples173 and 178 with structures and names as set forth in the Examplessection below.

EXAMPLES

Abbreviations Term Acronym High-pressure liquid chromatography HPLC Thinlayer chromatography TLC Diisopropylethylamine DIPEA Tetrahydrofuran THFDimethyl sulfoxide DMSO Benzyl alcohol BnOH n-Butyl alcohol nBuOHtert-Butylcarbamoyl Boc Di- tert-butyl dicarbonate BOC₂O DichloromethaneDCM Trifluoroacetic acid TFA Acetic Acid HOAc, AcOHN,N-Dimethylformamide DMF Methanol MeOH Ethanol EtOH Acetonitrile ACNEthyl Acetate EtOAc, or EA Triethylamine TEA Methanesulfonyl chlorideMsCl 2-(1H-9-Azobenzotriazole-1-yl)-1,1,3,3- HATU tetramethylaminiumhexafluorophosphate N,N,N′,N′-Tetramethyl-O-(1H- HBTUbenzotriazol-1-yl)uronium hexafluorophosphate 1-Ethyl-3-(3- EDCIdimethylaminopropyl)carbodiimide Hydroxybenzotriazole HOBt MethylTertiary Butyl Ether MTBE Diisopropyl azodicarboxylate DIADDiphenylphosphoryl azide DPPA Toluene PhCH₃ N-Methyl-2-pyrrolidone NMP4,5-Bis(diphenylphosphino)-9,9- Xantphos dimethylxanthene1,2,3,4,5-Pentaphenyl-1′-(di-t- CTC-Q-Phos butylphosphino)ferrocene orQPHos Bis(dibenzylideneacetone)palladium(0) Pd(dba)₂Tris(dibenzylideneacetone)dipalladium(0) Pd₂(dba)₃1,1′-Bis(di-tert-butylphosphino)ferrocene PdCl₂(dtbpf) palladiumdichloride 1-Propanephosphonic anhydride or T3P2,4,6-Tripropyl-1,3,5,2,4,6- trioxatriphosphorinane-2,4,6-trioxideSupercritical fluid chromatography SFC Room temperature rtChemistry:

In obtaining the compounds described in the examples below and thecorresponding analytical data, the following experimental and analyticalprotocols were followed unless otherwise indicated.

Unless otherwise stated, reaction mixtures were magnetically stirred atroom temperature (rt) under a nitrogen atmosphere. Where solutions were“dried,” they were generally dried over a drying agent such as Na₂SO₄ orMgSO₄, filtered and concentrated. Where mixtures, solutions, andextracts were “concentrated”, they were typically concentrated on arotary evaporator under reduced pressure. Reactions under microwaveirradiation conditions were carried out in a Biotage Initiator or CEMDiscover instrument.

Melting point determinations were performed in open capillary tubes on aFP62 or MP50 apparatus (Mettler-Toledo). Melting points were measuredwith a temperature gradient of 10° C./minute. Maximum temperature was300° C. The melting point was read from a digital display.

Normal-phase flash column chromatography (FCC) was performed on silicagel (SiO₂) using prepackaged cartridges, eluting with the indicatedsolvents.

Where compounds were purified by “Prep HPLC” the method employed waseither:

Preparative reverse-phase high performance liquid chromatography (HPLC)was performed on a Gilson HPLC with an Xterra Prep RP₁₈ (5 μm, 30×100mm, or 50×150 mm) column, and a gradient of 10 to 99% acetonitrile/water(20 mM NH₄OH) over 12 to 18 min, and a flow rate of 30 mL/min.

or

Preparative reverse-phase high performance liquid chromatography (HPLC)was performed on a Agilent 1100 Series HPLC with an XBridge C18 column(5 μm, 30×100 mm), mobile phase of 5% ACN in 20 mM NH4OH (hold for 2min) then ramp 5-99% ACN over 15 min, hold at 99% ACN for 5 min. and aflow rate of 40 mL/min.

or

Preparative reverse-phase high performance liquid chromatography (HPLC)was performed on a Agilent 1100 Series HPLC with an XBridge C18 column(5 μm, 50×100 mm), mobile phase of 5% ACN in 20 mM NH4OH (hold for 2min) then ramp 5-99% ACN over 15 min, hold at 99% ACN for 5 min. and aflow rate of 80 mL/min.

or

Preparative reverse-phase high performance liquid chromatography (HPLC)was performed on a Gilson HPLC with an Xterra Prep RP₁₈ (5 μm, 30×100mm, or 50×150 mm) column, and a gradient of 10 to 99% acetonitrile/water(20 mM NH₄OH) over 12 to 18 min, and a flow rate of 30 mL/min.

Analytical chromatography data was acquired using an Agilent 1100 HPLC,with an Inertsil ODS-3 3 mm 4.6×50 mm column, purchased from GL Sciences(Part #1010L050W046). Samples were run using a gradient profile of10-99% acetonitrile (ACN) in water, each containing 0.05%trifluoroacetic acid (TFA) over 1.6 minutes, then holding at 99%acetonitrile for 0.3 minutes. Flow rate was 5 mL/min and columntemperature was set to 50° C. (Method A).

Mass spectra (MS) were obtained on an Agilent series 1100 MSD usingelectrospray ionization (ESI) in positive mode unless otherwiseindicated. Calculated (calcd.) mass corresponds to the exact mass.

Nuclear magnetic resonance (NMR) spectra were obtained on Bruker modelDRX spectrometers. The format of the 1H NMR data below is: chemicalshift in ppm downfield of the tetramethylsilane reference (multiplicity,coupling constant J in Hz, integration). Definitions for multiplicityare as follows: s=singlet, d=doublet, t=triplet, q=quartet, m=multiplet,br=broad. For compounds that are present as a mixture of rotamers theratio is represented so that the total is 1, e.g. 0.80:0.20.Alternatively, 1H NMR data may be reported for only the major rotamer asindicated, or the data may be reported for one or more rotamers suchthat the total is less than 1. It will be understood that for compoundscomprising an exchangeable proton, said proton may or may not be visibleon an NMR spectrum depending on the choice of solvent used for runningthe NMR spectrum and the concentration of the compound in the solution.

Chemical names were generated using ChemDraw Ultra 12.0 (CambridgeSoftCorp., Cambridge, Mass.) or ACD/Name Version 10.01 (Advanced Chemistry).

Where compounds were purified by “SFC Chromatography” the methodemployed was either:

on a preparative APS 1010 system with autoprep motion from Bergerinstrument, consisted of two varian SD-1 pumps (walnut creek, CA, USA),one of which was extensively modified to pump CO₂, a special pump headheat exchanger, a julabo FT 401 chiller (labortechnik GmbH, Sellback,Germany), a model SCM 2500 phase separator (berger instruments) withselection valve and set of collection vessels in a Bodan robot. A modelKnauer 2500 UV detector with high pressure flow cell (berlin, germany).Samples were applied using a six-port injection valve (Valco, Houston,Tex., USA)) with a 5 mL sample loop and a model YP-300 syringue pump(cavro, san Jose, Calif.).

or

On a SFC-PICLAB-PREP 200 (PIC SOLUTION, Avignon, France). Modifier waspump with a model K1800 Knauer (Berlin, germany), with 100 mL Pump Head.The CO₂ was pump with 2 lewa pumps (Leonberg Germany). Cooling of thepump head and the CO₂ line was achieved by a coil alimented by a Huberchiller (Offenburg/Germany). Sample injections were made using 6switching valves (Valco, Houston, Tex., USA) and a 5 mL sample loop. Thesystem is managed by a PLC automation system.

Examples 114-115, 117-123, 126-127, 140, 141, 144-146, 225-264, 266-273are suitable for preparation using methods analogous to the methodsdescribed in the synthetic schemes and in the Examples section.

All stereochemistries depicted above and in Table 1 and Table 2 and inthe Examples section are relative. A notation of (±) or R/S indicatesthat the product is a racemic mixture of diastereomers. A notation of,for example, (2S,3R) indicates that the starting material was opticallypure and the product stereochemistry depicted is based on the knownstereochemistry of the starting material. A notation of, for example,(2S*,3R*) indicates that the product is a pure and single diastereomerbut the absolute stereochemistry is not established and relativestereochemistry is shown.

INTERMEDIATES

Inter- mediate Name Structure Reference A-1  4-fluoro-2- (2H-1,2,3-triazol-2- yl)benzoic acid

Prepared according to WO 2011/050198 Intermediate 4. A-2  2-fluoro-6-(pyrimidin- 2-yl)benzoic acid

WO 2011/050198 Intermediate 14. A-3  2-fluoro-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 5. A-4  2-(2H- 1,2,3- triazol-2- yl)benzoicacid

WO 2011/050198 Intermediate 2. A-5  5-methyl-2- (pyrimidin- 2-yl)benzoicacid

WO 2011/050198 Intermediate 50. A-6  5-methyl-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 8. A-7  5-fluoro-2- (pyrimidin- 2-yl)benzoicacid

WO 2011/050198 Intermediate 13. A-8  6-methyl-2- (2H-1,2,3- triazol-2-yl)nicotinic acid

WO 2011/050198 Intermediate 70 A-9  6-methyl-2- (1H-1,2,3- triazol-1-yl)nicotinic acid

WO 2011/050198 Intermediate 71 A-10 4-methoxy-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 54 A-11 4-methyl-2- (2H-1,2,3- triazol-2-yl)benzoic acid

Analogous Intermediate A-4 using 2-bromo-4- methylbenzoic acid A-123-ethoxy-6- methyl- picolinic acid

WO 2010/063663 Description 39 A-13 5-chloro-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 9. A-14 5-methoxy-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 10. A-15 3-methyl-2- (2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 82. A-16 3-(2H-1,2,3- triazol-2-yl)picolinic acid

WO 2011/050198 Intermediate 72. A-17 5-methyl-3- (oxazol-2- yl)picolinicacid

Prepared analogous to intermediate A-18 A-18 6-methyl-3- (oxazol-2-yl)picolinic acid

Prepared as described below A-19 6-methyl-3- (pyrimidin-2- yl)picolinicacid

WO 2010/063663 Description 69 A-20 5-methyl-3- (pyrimidin-2-yl)picolinic acid

Prepared analogous to intermediate A-19 using 3-bromo-5- methylpico-linonitrile

Intermediate A-18: 6-methyl-3-(oxazol-2-yl)picolinic acid

Step A: 3-bromo-6-methylpicolinic acid

To 3-bromo-6-methylpicolinonitrile (4 g, 20.3 mmol) in EtOH (40 mL) in asealed tube was added aqueous 4M NaOH (15 mL). The reaction was heatedat 90° C. for 24 h. Additional aqueous 4M NaOH was added and heatingcontinued at 90° C. for 24 h. The reaction was cooled to rt, acidifiedto pH=3 with 1N HCl (aq), concentrated and used without furtherpurification in subsequent steps. MS (ESI) mass calcd. for C₇H₆BrNO₂,216.0. m/z found 218 [M+H]⁺.

Step B: Methyl 3-bromo-6-methylpicolinate

To the title compound of step A (10.3 g, 20 mmol) in MeOH (50 mL) wasadded thionyl chloride (4.4 mL, 60 mmol). The reaction was heated atreflux overnight, cooled to rt and concentrated. Purification via silicagel chromatography (0-15% EtOAc in heptane) gave the title compound (1.9g, 40%). MS (ESI) mass calcd. for C₈H₈BrNO₂, 230.1. m/z found 232[M+H]⁺.

Step C: Methyl 6-methyl-3-(oxazol-2-yl)picolinate

In a microwave vial was dissolved the title compound of step B (185 mg,0.8 mmol) and 2-(tributylstannyl)oxazole (0.22 mL, 1 mmol) in PhCH₃ (2.4mL). The solution was degassed with N₂ and Pd(PPh₃)₄ (92 mg, 0.1 mmol)were added. The reaction was purged with N₂ and heated at 120° C. for 20min using microwave irradiation. The reaction was cooled to rt, filteredthrough a pad of celite and purified via silica gel chromatography(0-40% EtOAc in hexanes) to give the title compound of step A (333 mg,67%). The mixture was diluted with H₂O and extracted with EtOAc. Thecombined organics were dried (MgSO₄) and purified via silica gelchromatography (0-50% EtOAc in heptane) to give the title compound (56mg, 32%). MS (ESI) mass calcd. for C₁₁H₁₀N₂O₃, 218.2. m/z found 219.1[M+H]⁺.

Step D: 6-methyl-3-(oxazol-2-yl)picolinic acid

To the title compound of step C (56 mg, 0.3 mmol) was added MeOH (0.6mL) and 2M NaOH_((aq)) (0.6 mL). After 1 h at rt, 1M HCl_((aq)) wasadded. The reaction mixture was concentrated to give the title compound(52 mg) that was used without further purification in subsequent steps.MS (ESI) mass calcd. for C₁₀H₈N₂O₃, 204.2. m/z found 205.1 [M+H]⁺.

Intermediate A-21: 5-methyl-3-(2H-1,2,3-triazol-2-yl)picolinic acid

Step A: 5-methyl-3-(2H-1,2,3-triazol-2-yl)picolinonitrile

To 2-bromo-5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridine (1.5 g, 7.6 mmol)in DMF (19 mL) was added K₂CO₃ (1.2 g, 8.4 mmol) and 2H-1,2,3-triazole(440 μL, 7.6 mmol). The mixture was heated to 100° C. for 16 h, cooledto rt and extracted with EtOAc (2×). The combined organics were dried(Na2SO4) and concentrated. Purification via silica gel chromatography(5-60% EtOAc in hexanes) gave the title compound (490 mg, 35%) and5-methyl-3-(1H-1,2,3-triazol-1-yl)picolinonitrile (387 mg, 27%).

Step B: (sodium 5-methyl-3-(2H-1,2,3-triazol-2-yl)picolinate)

To a solution of the title compound of Step A (489 mg, 2.6 mmol) in EtOH(7 mL) was added 4 N NaOH (660 μL, 2.6 mmol). The mixture was heated at100° C. for 24 h. The reaction mixture was concentrated in vacuo to awhite solid which was used without further purification in subsequentsteps.

Intermediate A-22: 6-methyl-3-(2H-1,2,3-triazol-2-yl)picolinic acid

Step A: 6-methyl-3-(2H-1,2,3-triazol-2-yl)picolinonitrile

To 3-bromo-6-methylpicolinonitrile (2.2 g, 11 mmol) in DMF (28 mL) wasadded K₂CO₃ (1.7 g, 12 mmol) and 2H-1,2,3-triazole (650 μL, 11 mmol).The mixture was heated to 100° C. for 36 h, cooled to rt and extractedwith EtOAc. The combined organics were dried (Na2SO4) and concentrated.Purification via silica gel chromatography (10-100% EtOAc in hexanes)gave the title compound (1 g, 48%).

Step B: 6-methyl-3-(2H-1,2,3-triazol-2-yl)picolinic acid

To a solution of the title compound of Step A (730 mg, 4 mmol) in EtOH(10 mL) was added 4 N NaOH (1 mL, 4 mmol). The mixture was heated at100° C. for 24 h. The reaction mixture was concentrated in vacuo to awhite solid which was used without further purification in subsequentsteps.

A-23 2-fluoro-6-(2H-1,2,3- triazol-2-yl)benzoic acid

Prepared according to WO 2011/050198 Intermediate 12 A-245-fluoro-2-(2H-1,2,3- triazol-2-yl)benzoic acid

WO 2011/050198 Intermediate 1

Intermediate A-25: 2-(5-fluoropyrimidin-2-yl)benzoic acid

Step A: 5-fluoro-2-iodopyrimidine

To a solution of 2-chloro-5-fluoropyrimidine (4 mL, 32 mmol) inpropionitrile (33 mL) was added chlorotrimethylsilane (12 mL, 97 mmol)and sodium iodide (15 g, 97 mmol), and the reaction mixture was heatedto 150° C. for 1 h. Upon completion of the reaction, the reactionmixture was cooled to room temperature and the solvent removed. Theresidue was taken up in EtOAc and a solution of saturated NaHCO₃. Theorganic layer was dried over MgSO₄, filtered and evaporated.Purification via silica gel chromatography (0-20% EtOAc in hexanes) gavethe title compound (2.82 g, 39%).

Step B: 2-(5-fluoropyrimidin-2-yl)benzonitrile

In a microwave vial was dissolved cyanophenylboronic acid (500 mg, 3.40mmol) in THF (15 mL), and the reaction mixture was degassed with N₂.Then, the title compound of step A (915 mg, 4.08 mmol), Na₂CO₃ (1.08 g,10.2 mmol), water (5 mL), and PdCl₂(dtbpf) (CAS 95408-45-0) (89 mg, 0.14mmol) were added, and the reaction mixture was stirred at roomtemperature for 1 h and then heated via microwave heating to 75° C. for2 h. The mixture was cooled to room temperature and water and EtOAcadded. The reaction mixture was extracted with EtOAc. The combinedorganic layers were dried over MgSO₄, filtered and concentrated. Thecrude was purified via silica gel chromatography (0-30% EtOAc inhexanes) to afford the title compound (280 mg, 41%). MS (ESI) masscalcd. for C₁₁H₆FN₃, 199.1. m/z found 200.0 [M+H]⁺.

Step C: 2-(5-fluoropyrimidin-2-yl)benzoic acid

A solution of the title compound of step B (1.24 g, 6.22 mmol) in H₂SO₄(6 mL) and water (6 mL) was stirred at 80° C. for 1 h. Then, thereaction mixture was cooled to 0° C. and the aqueous phase extractedwith DCM (2×). A solution of 20 M NaOH (11 mL) was added to the aqueouslayer until pH ˜3-4. The aqueous layer was extracted again with EtOAcand DCM. The combined organic layers were dried over MgSO₄, filtered andconcentrated to afford the title compound (672 mg, 50%). MS (ESI) masscalcd. for C₁₁H₇FN₂O₂, 218.1. m/z found 219.1 [M+H]⁺.

Intermediate A-26: 3-fluoro-2-(5-fluoropyrimidin-2-yl)benzoic acid

Prepared analogous to Intermediate A-25, substituting cyanophenylboronicacid with (2-cyano-6-fluorophenyl)boronic acid (CAS 656235-44-8). MS(ESI) mass calcd. for C₁₁H₆F₂N₂O₂, 236.0. m/z found 237.1 [M+H]⁺.

Intermediate A-27: 3-methyl-2-(pyrimidin-2-yl)benzoic acid

Step A: methyl 3-methyl-2-(pyrimidin-2-yl)benzoate

In a microwave vial was dissolved methyl3-methyl-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoate (619mg, 2.24 mmol) and 2-chloropyrimidine (314 mg, 2.69 mmol) in 2-MeTHF (10mL). Na₂CO₃ (713 mg, 6.73 mmol) was then added followed by water (3.4mL) and the reaction mixture was degassed with N₂ for 45 minutes.Pd(dppf)Cl₂ (66 mg, 0.09 mmol) and the reaction mixture was heated at75° C. for 28 h. More Pd(dppf)Cl₂ (33 mg, 0.045 mmol) was added and thereaction mixture was heated at 150° C. for 3.5 h. The mixture wasfiltered through a pad of celite and the filter cake rinsed with EtOAcand water. The layers were separated and the aqueous was extracted oncewith EtOAc. The combined organic layers were dried over MgSO₄, filteredand evaporated. The crude was purified via silica gel chromatography(0-50% EtOAc in hexanes) to afford the title compound (116 mg, 23%). MS(ESI) mass calcd. for C₁₃H₁₂N₂O₂, 228.1. m/z found 229.1 [M+H]⁺. ¹H NMR(500 MHz, CDCl3) 8.95-8.76 (m, 2H), 7.99-7.75 (m, 1H), 7.50-7.44 (m,1H), 7.43-7.37 (m, 1H), 7.32-7.24 (m, 1H), 3.64 (s, 3H), 2.15 (s, 3H).

Step B: 3-methyl-2-(pyrimidin-2-yl)benzoic acid

To a solution of the title compound of step A (980 mg, 4.29 mmol) inMeOH (21 mL) was added 10 M NaOH (2 mL), and the reaction mixture wasstirred at 50° C. for 48 h. The solvent was removed and the crude wasdiluted with water and acidified with 6 M HCl_((aq)) until pH=3. Theaqueous layer was saturated with solid NaCl and extracted with 20% iPrOHin CHCl₃ (3×). The combined organic layers were dried over MgSO₄,filtered and concentrated to afford the title compound (892 mg, 96%). MS(ESI) mass calcd. for C₁₂H₁₀N₂O₂, 214.1. m/z found 215.1 [M+H]⁺.

Intermediate Name Structure Reference A-28 3-fluoro-2-(pyrimidin-2-yl)benzoic acid

Prepared according to WO 2011/050198 Intermediate 50 A-29 2-pyrimidin-2-yl)benzoic acid

Commercially available, CAS 400892-62-8 A-30 5-methyl-2-(2H-1,2,3-triazol-2- yl)nicotinic acid

Prepared analogous to WO 2011/050200 Intermediate 47, Example 160 A-312-(2H-1,2,3- triazol-2- yl)nicotinic acid

Commercially available, CAS 136497-44-8 A-32 2-chloro-6-methoxynicotinic acid

Commercially available, CAS 1227515-71-0 A-33 3-phenylpyrazine-2-carboxylic acid

Commercially availble, CAS 2881- 85-8

Synthesis of 3-fluoro-2-(pyrimidin-2-yl)benzonitrile (Intermediate inthe synthesis of intermediate A-28)

To a solution of3-fluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzonitrile(4.98 g, 19.1 mmol) and 2-bromopyrimidine (3.85 g, 23 mmol) in THF (96mL) was added Na₂CO₃ (6 g, 57.4 mmol) followed by water (43 mL). Thereaction mixture was degassed with N₂ for 10 minutes. PdCl₂(dtbpf) (374mg, 0.57 mmol) was added and the reaction mixture was stirred at 80° C.for 5 h. The solution was cooled to room temperature and a mixture ofEtOAc and water was added. The aqueous was extracted twice with EtOAcand the combined organic layers were dried over MgSO4, filtered andevaporated. The title compound was precipitated by dissolving theresidue in a minimum amount of EtOAc and then adding hexanes. The solidwas filtered, washed with hexanes and dried to afford the title compound(2.46 g, 64%). MS (ESI) mass calcd. for C₁₁H₆FN₃, 199.1. m/z found 200.1[M+H]⁺. ¹H NMR (400 MHz, Chloroform-d) δ 9.02-8.91 (m, 2H), 7.65 (dt,J=7.7, 1.0 Hz, 1H), 7.60-7.52 (m, 1H), 7.51-7.43 (m, 1H), 7.41 (t, J=4.9Hz, 1H).

Intermediate A-34: 4-fluoro-2-(oxazol-2-yl)benzoic acid

Step A: 2-bromo-N-(2,2-dimethoxyethyl)-6-fluorobenzamide

To a solution of 2-bromo-6-fluorobenzoic acid (2 g, 9.1 mmol) in DMF (27mL) was added HBTU (5.20 g, 13.7 mmol) and DIPEA (4.7 mL, 27 mmol), andthe reaction mixture was stirred for 10 min. Then,2,2-dimethoxyethylamine (1.3 mL, 11.9 mmol) was added and the reactionmixture stirred at room temperature for 12 h. The reaction mixture wasdiluted with EtOAc and washed with saturated aqueous NaHCO₃. Thecombined organic layers were dried over MgSO₄, filtered andconcentrated. Purification via silica gel chromatography (0-25% EtOAc inhexanes) gave the title compound (2.3 g, 82%).

Step B: 2-(2-bromo-6-fluorophenyl)oxazole

To P₂O₅ (6.4 g, 22.6 mmol) was added methanesulfonic acid (52 mL, 801mmol), and the reaction mixture was stirred at room temperature for 1 h.Then, the title compound of step A (2.3 g, 7.54 mmol) was added to thereaction mixture, and the mixture heated to 140° C. for 2 h. DCM wasadded and the mixture was slowly poured into a saturated solution ofaqueous NaHCO₃ on ice. The mixture was extracted with DCM. The combinedorganic layers were dried over MgSO₄, filtered and concentrated.Purification via silica gel chromatography (0-10% EtOAc in hexanes) gavethe title compound (1.5 g, 82%). MS (ESI) mass calcd. for C₉H₅BrFNO,240.95. m/z found 242.0 [M+H]⁺.

Step C: Methyl 3-fluoro-2-(oxazol-2-yl)benzoate

A solution of the title compound of step B (2.18 g, 8.99 mmol), Pd(OAc)₂(40 mg, 0.18 mmol), 1,1′-bis(diphenylphosphino)ferrocene (199 mg, 0.36mmol), and Et₃N (3.7 mL, 27 mmol) in 1:1 MeOH/1,4-dioxane (36 mL) wasdegassed with N₂ for 15 min. Then, the mixture was stirred at 95° C.under an atmosphere of carbon monoxide overnight. The reaction mixturewas diluted with EtOAc and washed with a solution of NaHCO₃. The organiclayer was separated, dried over MgSO₄, filtered, and concentrated.Purification via silica gel chromatography (0-12% EtOAc in hexanes) gavethe title compound (1.7 g, 83%). MS (ESI) mass calcd. for C₁₁H₈FNO₃,221.1. m/z found 222.0 [M+H]⁺.

Step D: 3-fluoro-2-(oxazol-2-yl)benzoic acid

To a solution of the title compound of step C (1.65 g, 7.46 mmol) inMeOH (22 mL) was added 2 M NaOH (7.5 mL), and the reaction mixture wasstirred at room temperature overnight. The reaction mixture wasacidified with 1 M HCl_((aq)) and the solvents evaporated in vacuo. Themixture was diluted with water and extracted with DCM. The combinedorganic were dried over MgSO₄, filtered and concentrated to afford thetitle compound (905 mg, 58%). MS (ESI) mass calcd. for C₁₀H₆FNO₃, 207.0.m/z found 208.0 [M+H]⁺. MP=182° C.

Intermediate A-35: 5-fluoro-2-(oxazol-2-yl)benzoic acid

Step A: Methyl 5-fluoro-2-(oxazol-2-yl)benzoate

To a solution of methyl 2-bromo-5-fluorobenzoate (1.1 g, 4.8 mmol) and2-(tri-n-butylstannyl)oxazole (1.3 mL, 6.2 mmol) in toluene (14 mL) wasadded Pd(PPh₃)₄ (550 mg, 0.476 mmol), and the reaction mixture washeated via microwave heating to 150° C. for 30 min. The reaction mixturewas diluted with water and extracted with EtOAc. The combined organiclayers were dried over MgSO₄, filtered and concentrated. Purificationvia silica gel chromatography (0-40% EtOAc in hexanes, followed by asecond column 0-10% EtOAc in hexanes) gave the title compound (553 mg,52%). MS (ESI) mass calcd. for C₁₁H₆FNO₃, 221.1. m/z found 222.1 [M+H]⁺.

Step B: 5-fluoro-2-(oxazol-2-yl)benzoic acid

Prepared analogous to intermediate 34, step D, to give the titlecompound (858 mg, 99%). MS (ESI) mass calcd. for C₁₀H₆FNO₃, 207.0. m/zfound 208.1 [M+H]⁺.

Intermediate A-36: 4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)benzoicacid

Step A: 5-(2-bromo-5-fluorophenyl)-3-methyl-1,2,4-oxadiazole

To a solution of 2-bromo-5-fluorobenzoyl chloride (2.17 g, 9.13 mmol) inTHF (18 mL) was added DIPEA (1.7 mL, 10 mmol). Then, acetamide oxime(676 mg, 9.13 mmol) was added portionwise, and the reaction mixture wasstirred at 70° C. for 16 h. The reaction mixture was diluted with EtOAcand washed with a saturated solution of NaHCO₃. The combined organiclayers were dried over MgSO₄, filtered and concentrated. Purificationvia silica gel chromatography (0-20% EtOAc in hexanes) gave the titlecompound (2.35 g, 57%). MS (ESI) mass calcd. for C₉H₆BrFN₂O, 255.96. m/zfound 257.0 [M+H]⁺.

Step B: 4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)benzoic acid

Prepared analogous to intermediate 34, steps C and D, to give the titlecompound. MS (ESI) mass calcd. for C₁₀H₇FN₂O₃, 222.0. m/z found 223.0[M+H]⁺.

Intermediate Name Structure Reference A-37 4-fluoro-2- (pyrimidin-2-yl)benzoic acid

Prepared according to WO 2011/050198 Intermediate 87 A-38 3-(pyrimidin-2-yl)picolinic acid

WO 2010/122151 Intermediate D105

Intermediate A-39: 5-methyl-2-(pyrimidin-2-yl)nicotinic acid

Step A: Methyl 5-methyl-2-(pyrimidin-2-yl)nicotinate

To a sealed tube containing methyl 2-chloro-5-methylnicotinate (CAS65169-43-9) (745 mg, 4.01 mmol), CuI (38 mg, 0.2 mmol), LiCl (169 mg,4.01 mmol), and Pd(PPh₃)₄ (231 mg, 0.2 mmol) in toluene (15 mL) wasadded 2-(tributylstannyl)pyrimidine (1.5 mL, 4.4 mmol), and the reactionmixture was heated at 120° C. overnight. The reaction mixture wasdiluted with water and extracted with DCM. The combined organic layerswere dried over MgSO₄, filtered and evaporated. Purification via silicagel chromatography (0-50% EtOAc in hexanes) gave the title compound (494mg, 52%). MS (ESI) mass calcd. for C₁₂H₁₁N₃O₂, 229.1. m/z found 229.99.

Step B: 5-methyl-2-(pyrimidin-2-yl)nicotinic acid

To a solution of the title compound of step A (466 mg, 2.03 mmol) inMeOH (10 mL) was added 10 M NaOH (1 mL), and the reaction mixture wasstirred at room temperature for 2 h. The solvent was removed and thecrude was diluted with water and acidified with 6 M HCl_((aq)) untilpH=3. The aqueous layer was saturated with solid NaCl and extracted with20% iPrOH in CHCl₃ (3×). The combined organic layers were dried overMgSO₄, filtered and concentrated to afford the title compound (432 mg,99%). MS (ESI) mass calcd. for C₁₁H₉N₃O₂, 215.1. m/z found 216.1 [M+H]⁺.¹H NMR (500 MHz, Methanol-d₄) δ 8.90 (br. s, 2H), 8.64 (br. s, 1H), 8.17(s, 1H), 7.55 (br. s, 1H), 2.51 (s, 3H).

Intermediate Name Structure Reference A-40 2-fluoro-6- (pyrimidin-2-yl)benzoic acid

Prepared according to WO 2011/050198 Intermediate 14 A-41 5-methyl-2-(2H-1,2,3- triazol-2- yl)nicotinic acid

Preparaed analogous to WO 2011/050200 Intermediate 47, Example 160

Intermediate B-1:trans-(±)-tert-butyl-3-(((benzyloxy)carbonyl)amino)-2-methylpiperidine-1-carboxylate

Step A:trans-(±)-1-(tert-butoxycarbonyl)-2-methylpiperidine-3-carboxylic acid

Methyl 2-methylnicotinate (28.6 g, 190 mmol) and PtO₂ (430 mg) in AcOH(500 mL) was stirred under an atmosphere of H₂ for 48 h. Additional PtO₂was added and the reaction continued for an additional 24 h. Then thereaction mixture was filtered through a pad of celite and the filtrateconcentrated. The resulting residue was partitioned between 2M NaOH (aq)and DCM. The organic layer was extracted with DCM (2×). The combinedorganic layers were dried (MgSO₄) and concentrated to give 27.2 g of aresidue.

To this residue in MeOH (450 mL) was added sodium methoxide (4.5 M inMeOH, 56 mL, 260 mmol). The reaction was heated at reflux for 15 h, thencooled to rt, concentrated, H₂O was added and the mixture extracted withDCM. The combined organics were dried (MgSO₄) and concentrated to give23.9 g of product that was dissolved in THF (300 mL) and H₂O (60 mL).Then, Na₂CO₃ (38.3 g, 456 mmol) was added followed by Boc₂O (33.2 g, 152mmol). After stirring overnight, the mixture was diluted with H₂O andextracted with DCM. The combined organics were dried (MgSO₄) andconcentrated to give 39.2 g of material (9:1 trans:cis isomers).

To this material in THF (300 mL) and H₂O (100 mL) was added LiOH (7.3 g,305 mmol). After stirring overnight, the mixture was diluted with H₂Oand extracted with DCM. The organic extract was discarded. The aqueouslayer was acidified with HCl and extracted with DCM. The combinedorganics were dried (MgSO₄) and concentrated. Purification via silicagel chromatography (0-100% EtOAc in heptane) gave the title compound(30.2 g) as a ˜86:13 mixture of trans:cis diastereomers. MS (ESI) masscalcd. for C₁₂H₂₁NO₄, 243.2. m/z found 188.1 [M−55]⁺.

Step B: trans-(±)-tert-butyl3-(((benzyloxy)carbonyl)amino)-2-methylpiperidine-1-carboxylate

To the title compound of step A (10 g, 41 mmol) in PhCH₃ (60 mL) wasadded DIPEA (7.4 mL, 43 mmol) and the mixture heated at 50° C. for 1 h.Then DPPA (8.9 mL, 41 mmol) in PhCH₃ was added dropwise and the mixtureheated at 75° C. for 2 h and BnOH (4.5 mL, 43 mmol) and the mixturestirred at 80° C. for 20 h. The reaction was allowed to cool to rt,diluted with saturated NaHCO₃ (aq) and extracted with PhCH3. Thecombined organics were dried (MgSO₄). Purification via silica gelchromatography (0-20% EtOAc in hexanes) gave the title compound (2.6 g,18%). MS (ESI) mass calcd. for C₁₉H₂₈N₂O₄, 348.2. m/z found 249.2[M−100]⁺, 293.2 [M−55]⁺. ¹H NMR (400 MHz, CDCl₃) δ 7.39-7.30 (m, 5H),5.24-5.00 (m, 3H), 4.35-4.22 (m, 1H), 4.01-3.85 (m, 1H), 3.74-3.51 (m,1H), 2.89-2.71 (m, 1H), 1.87-1.30 (m, 13H), 1.19 (d, J=7.0 Hz, 3H).

Example B-2 (2R*,3S*)-tert-butyl3-(((benzyloxy)carbonyl)amino)-2-methylpiperidine-1-carboxylate

and (2S*,3R*)-tert-butyl3-(((benzyloxy)carbonyl)amino)-2-methylpiperidine-1-carboxylate

The title compounds were obtained by chiral SFC (CHIRALPAK AD-H 5 μM250×30 mm) resolution of Intermediate B-1 (38.2 g) using 93% CO₂/7% MeOHas the mobile phase to give enantiomer A (18.8 g, 1st elutingenantiomer) and enantiomer B (19.3 g, 2^(nd) eluting enantiomer).Enantiomer A: [α]_(D) ²⁰−31.9° (c 0.7, DMF). MS (ESI) mass calcd. forC₁₉H₂₈N₂O₄, 348.2. m/z found 349.2 [M+H]⁺. 1H NMR consistent withintermediate B-1.Enantiomer B: [α]_(D) ²⁰+28.0° (c 0.96, DMF). MS (ESI) mass calcd. forC₁₉H₂₈H₂₈N₂O₄, 348.2. m/z found 349.2 [M+H]⁺. 1H NMR consistent withintermediate B-1.

Intermediate B-3:trans-(±)-tert-butyl-3-amino-2-methylpiperidine-1-carboxylate

Intermediate B-1 (8.9 g, 25.4 mmol) and wet 10 wt % Pd/C (880 mg) inMeOH (200 mL) was stirred under an atmosphere of H₂ overnight. Thereaction mixture was filtered through a pad of celite and the filtrateconcentrated to give the title compound (5.7 g) that was used withoutfurther purification. MS (ESI) mass calcd. for C₁₁H₂₂N₂O₂, 214.3. m/zfound 159.1 [M−55]⁺. ¹H NMR (400 MHz, CDCl₃) 4.19-4.08 (m, 1H),4.00-3.88 (m, 1H), 2.91-2.72 (m, 2H), 1.86-1.61 (m, 1H), 1.61-1.33 (m,12H), 1.15 (d, J=7.0 Hz, 3H).

Intermediate B-4: (2S*,3R*)-tert-butyl3-amino-2-methylpiperidine-1-carboxylate

Prepared analogous to Intermediate B-3 substituting intermediate B-1with intermediate (+)-B-2 (enantiomer B).

Intermediate B-5: (2R*,3S*)-tert-butyl3-amino-2-methylpiperidine-1-carboxylate

Prepared analogous to Intermediate B-3 substituting intermediate B-1with intermediate (−)-B-2 (enantiomer A).

Intermediate B-6:cis-(±)-tert-butyl-3-amino-2-methylpiperidine-1-carboxylate

Step A: cis-(±)-1-(tert-butoxycarbonyl)-2-methylpiperidine-3-carboxylicacid

Methyl 2-methylnicotinate (100 g, 662 mmol) and PtO₂ (1.5 g) in AcOH(300 mL) was stirred under an atmosphere of H₂ for 7 days. The reactionmixture was filtered and the filtrate concentrated. The resultingresidue was partitioned between saturated NaHCO₃ (aq) and DCM. Theorganic layer was extracted with DCM (4×200 mL). The combined organiclayers were dried (Na₂SO₄) and concentrated to give 80 g of a residue.

To this residue in THF (200 mL) was added DIPEA (37 mL, 200 mmol) andBoc₂O (32 g, 150 mmol). After stirring overnight, the mixture wasconcentrated, diluted with H₂O and extracted with EtOAc. The combinedorganics were dried (NaSO₄) and concentrated. Purification via silicagel chromatography (10% EtOAc in petroleum ethers) gavecis-(±)-1-tert-butyl 3-methyl 2-methylpiperidine-1,3-dicarboxylate.

To the crude mixture of cis-(±)-1-tert-butyl3-methyl-2-methylpiperidine-1,3-dicarboxylate from above in MeOH (150mL) was added 2M NaOH (aq.). The reaction was heated at 70° C. for 2 h,cooled to rt, concentrated, treated with H₂O (400 mL) and extracted withMTBE (100 mL). The aqueous layer was then acidified to pH=6 with 1N HCl(aq) and concentrated. To this was added MeOH (400 mL) and concentratedto give the title compound (5 g) as a white solid.

Step B: cis-(±)-tert-butyl3-(((benzyloxy)carbonyl)amino)-2-methylpiperidine-1-carboxylate

To the title compound of step A (5.3 g, 21 mmol) in PhCH₃ was addedDIPEA (4.3 mL, 26 mmol) and DPPA (7.2 g, 26 mmol). The mixture washeated at reflux overnight, cooled to rt, concentrated and treated with20% KOH (aq). The mixture was heated at 100° C. for 2 h, cooled to rtand extracted with DCM (2×). The combined organics were dried (Na2SO4),concentrated and purified via silica gel chromatography (10% MeOH inDCM) to give the title compound (1.5 g).

Example 1(±)-trans-(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Step A:(±)-tert-butyl-3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylate

To a microwave vial was weighed intermediate B-3 (260 mg, 1.2 mmol),2-chloro-4,6-dimethylpyrimidine (208 mg, 1.5 mmol), Pd(dba)₂ (17 mg,0.03 mmol), CTC-Q-Phos (27 mg, 0.06 mmol) and sodium tert-butoxide (175mg, 1.8 mmol). The vial was capped, evacuated, refilled with N₂ (2×) andPhCH₃ was added. The reaction was then heated in the microwave at 150°C. for 120 min. The mixture was then cooled to rt, directly applied toand purified via silica gel chromatography (1-7% 2M NH₃/MeOH in CH₂Cl₂)to give the title compound (205 mg, 53%) as a brown oil. MS (ESI) masscalcd. for C₁₂H₂₈N₄O₂, 320.2. m/z found 321.2 [M+H]⁺. 1H (CDCl3): 6.30(s, 1H), 5.39-5.25 (m, 1H), 4.46-4.31 (m, 1H), 4.10-3.85 (m, 2H),2.94-2.71 (m, 1H), 2.34-2.20 (m, 6H), 1.87-1.62 (m, 3H), 1.52-1.33 (m,10H), 1.22 (d, J=7.0 Hz, 2.5H), 1.09-1.01 (m, 0.5H).

Step B:(±)-trans-4,6-dimethyl-N-(2-methylpiperidin-3-yl)pyrimidin-2-amine

To the title compound from Step A (75 mg, 0.23 mmol) in DCM (4 mL) wasadded TFA (4 mL). Upon completion (˜3 h), the reaction was concentrated,neutralized with 5% Na₂CO₃ (aq) and extracted with DCM (3×). Thecombined organics were dried (Na₂SO₄) to give the title compound (52 mg)which was used without further purification. MS (ESI) mass calcd. forC₁₂H₂₀N₄, 220.2. m/z found 221.1 [M+H]⁺.

Step C:(±)-trans-(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

To the title compound from Step B (29 mg, 0.13 mmol) in DMF (1.3 mL) wasadded TEA (0.022 mL, 0.16 mmol), intermediate A-1 (30 mg, 0.14 mmol) andHATU (55 mg, 0.14 mmol). Upon completion of the reaction, purificationwas performed using Agilent prep method A to give the title compound (29mg, 55% yield). MS (ESI) mass calcd. for C₂₁H₂₄FN₇O, 409.2. m/z found410.0 [M+H]⁺. HPLC R_(t)=0.85 (Analytical Method A).

Example 2(±)-trans-(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 1 substitutingintermediate A-1 with intermediate A-2. MS (ESI) mass calcd. forC₂₃H₂₅FN₆O, 420.2. m/z found 421.2 [M+H]⁺.

Example 3(±)-trans-(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to example 1 substitutingintermediate A-1 with intermediate A-23. MS (ESI) mass calcd. forC₂₁H₂₂FN₇O, 407.5. m/z found 408.2 [M+H]⁺. HPLC R_(t)=0.85 (AnalyticalMethod A).

Example 4(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

The title compound was prepared analogous to example 1 substitutingintermediate A-1 with intermediate A-4. MS (ESI) mass calcd. forC₂₁H₂₅N₇O, 391.2. m/z found 392.2 [M+H]⁺.

Example 5(±)-trans-(3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 1 substitutingintermediate A-1 with intermediate A-24. MS (ESI) mass calcd. forC₂₁H₂₄FN₇O, 409.2. m/z found 410.2 [M+H]⁺.

Example 6(±)-trans-(3-((4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 1 substitutingintermediate A-1 with intermediate A-5. MS (ESI) mass calcd. forC₂₄H₂₈N₆O, 416.2. m/z found 417.2 [M+H]⁺.

Example 7(±)-trans-(5-methyl-2-(pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl-2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone

Step A:(±)-tert-butyl-2-methyl-3-((6-methyl-2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidine-1-carboxylate

To intermediate B-3 (135 mg, 0.63 mmol) in DMF (1.5 mL) was added Cs₂CO₃(308 mg, 0.95 mmol) and 4-chloro-6-methyl-2-(trifluoromethyl)pyrimidine(149 mg, 0.76 mmol). The flask was then heated with an oil bath at 70°C. for 18 h. The reaction was allowed to cool to rt, diluted with H₂Oand extracted with EtOAc (2×). The combined organics were washed withbrine and dried (Na₂SO₄). Silica gel chromatography (EtOAc in hexanes)gave the title compound (111 mg, 47%). MS (ESI) mass calcd. forC₁₇H₂₅F₃N₄O₂, 374.2. m/z found 275.2 [M+H-100]+. 1H NMR (CDCl3): 6.24(s, 1H), 5.44 (s, 1H), 4.48-4.31 (m, 1H), 4.15-3.60 (m, 2H), 2.97-2.78(m, 1H), 2.42 (s, 3H), 1.85 (s, 2H), 1.74-1.32 (m, 10H), 1.26 (d, J=7.0Hz, 3H).

Step B:(±)-trans-6-methyl-N-(2-methylpiperidin-3-yl)-2-(trifluoromethyl)pyrimidin-4-amine

The title compound was prepared analogous to example 1, Step Bsubstituting(±)-tert-butyl-3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylatewith the title compound from Step A.

Step C:(±)-trans-(5-methyl-2-(pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl-2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to example 1, Step Csubstituting intermediate A-1 with intermediate A-5 and(±)-4,6-dimethyl-N-(2-methylpiperidin-3-yl)pyrimidin-2-amine with thetitle compound of Step B. MS (ESI) mass calcd. for C₂₄H₂₅F₃N₆O, 470.2.m/z found 471.2 [M+H]⁺.

Example 8(±)-trans-(5-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((6-methyl-2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 7 substitutingintermediate A-5 with intermediate A-6. MS (ESI) mass calcd. forC₂₂H₂₄F₃N₇O, 459.2. m/z found 460.2 [M+1-1]⁺.

Example 9(±)-trans-(5-fluoro-2-(pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl-2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 7 substitutingintermediate A-5 with intermediate A-7. MS (ESI) mass calcd. forC₂₃H₂₂F₄N₆O, 474.2. m/z found 475.2 [M+1-1]⁺.

Example 10(±)-trans-(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((4-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 7 substituting4-chloro-6-methyl-2-(trifluoromethyl)pyrimidine with2-chloro-4-(trifluoromethyl)pyrimidine and intermediate A-5 withintermediate A-23. MS (ESI) mass calcd. for C₂₀H₁₉F₄N₇O, 449.2. m/zfound 450.2 [M+H]⁺.

Example 11(±)-trans-(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)(2-methyl-3-((4-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 10 substitutingintermediate A-23 with intermediate A-9. MS (ESI) mass calcd. forC₂₀H₂₁F₃N₈O, 446.2. m/z found 447.2 [M+H]⁺.

Example 12(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Step A:trans-(±)-tert-butyl-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidine-1-carboxylate

To intermediate B-3 (3 g, 14 mmol) in DMSO (100 mL) was added2-fluoro-5-(trifluoromethyl)pyridine (3.4 g, 21 mmol) and DIPEA (4.8 mL,28 mmol). The reaction was then heated at 100° C. for 4 h. The reactionwas allowed to cool to rt, diluted with saturated NaHCO₃ (aq) andextracted with DCM. The combined organics were washed with brine anddried (MgSO₄). Silica gel chromatography (EtOAc in heptane) gave thetitle compound (2.7 g, 54%). MS (ESI) mass calcd. for C₁₇H₂₄F₃N₃O₂,359.2. m/z found 360.2 [M+H]⁺.

Step B.trans-(±)-2-methylpiperidin-3-yl)-5-(trifluoromethyl)pyridin-2-amine

Prepared analogous to Example 1 substituting(±)-tert-butyl-3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylatewith the title compound from Step A. MS (ESI) mass calcd. forC₁₂H₁₆F₃N₃, 259.1. m/z found 260.2 [M+H]⁺.

Step C.(±)-trans-tert-butyl-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidine-1-carboxylate

Prepared analogous to Example 1 substituting(±)-trans-(3-((4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanonewith the title compound from Step B, intermediate A-1 with intermediateA-4 and HATU with HBTU. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O, 430.2. m/zfound 431.2 [M+H]⁺. MP=154.7° C. (Mixture of 4 isomers, undefinedratio). ¹H NMR (300 MHz, DMSO) δ 8.36 (d, J=21.8 Hz, 0.44H), 8.24 (d,J=12.9 Hz, 0.36H), 8.08 (d, J=1.3 Hz, 1.62H), 7.97 (s, 0.47H), 7.91 (d,J=8.2 Hz, 0.41H), 7.83-7.32 (m, 4.20H), 7.32-7.21 (m, 0.48H), 7.06-6.96(m, 0.47H), 6.82 (d, J=8.9 Hz, 0.76H), 6.71 (dd, J=13.9, 7.0 Hz, 0.59H),6.60 (s, 0.10H), 6.15 (d, J=8.8 Hz, 0.10H), 4.93 (d, J=6.0 Hz, 0.12H),4.68 (q, J=6.8 Hz, 0.39H), 4.41 (t, J=13.4 Hz, 0.65H), 4.17-3.83 (m,0.78H), 3.81-3.61 (m, 0.73H), 3.20 (d, J=14.4 Hz, 0.35H), 2.94 (t,J=11.7 Hz, 0.52H), 2.85-2.66 (m, 0.46H), 2.19-1.49 (m, 3.53H), 1.49-1.22(m, 2.07H), 0.74 (d, J=6.9 Hz, 1.40H).

Example 13(±)-trans-(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogously to Example 12 substituting intermediate A-4 withintermediate A-11. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O, 444.2. m/zfound 445.2 [M+H]+. MP=136.4° C. (Mixture of 4 isomers, undefinedratio). 1H NMR (300 MHz, DMSO) δ 8.41 (d, J=20.3 Hz, 0.34H), 8.28 (d,J=13.4 Hz, 0.34H), 8.21-7.94 (m, 2.16H), 7.88-7.67 (m, 1.27H), 7.60 (dd,J=12.3, 7.4 Hz, 0.92H), 7.53-7.21 (m, 1.81H), 6.96-6.72 (m, 1.41H), 6.60(s, 0.11H), 6.51 (d, J=7.4 Hz, 0.53H), 6.17 (s, 0.11H), 4.96 (s, 0.11H),4.72 (d, J=7.3 Hz, 0.34H), 4.42 (d, J=14.1 Hz, 0.68H), 4.11 (s, 0.45H),3.98 (s, 0.20H), 3.77 (d, J=6.8 Hz, 0.56H), 3.67 (dd, J=12.1, 6.0 Hz,0.68H), 3.26 (d, J=13.4 Hz, 0.34H), 2.96 (t, J=11.7 Hz, 0.50H),2.86-2.71 (m, 0.50H), 2.49 (s, 1.07H), 2.43 (s, 0.33H), 2.26 (s, 1.24H),2.18-1.52 (m, 3.57H), 1.53-1.33 (m, 0.90H), 1.30 (d, J=7.0 Hz, 0.94H),0.76 (d, J=6.9 Hz, 1.57H).

Example 14(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogously to Example 13 substituting intermediate B-3 withintermediate B-4. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O, 444.2. m/z found445.2 [M+H]⁺. MP=119.4° C.

Example 15(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2R,3S)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogously to Example 13 substituting intermediate B-3 withintermediate B-5. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O, 444.2. m/z found445.2 [M+H]⁺. MP=130.4° C. (Mixture of 4 isomers, undefined ratio). ¹HNMR (300 MHz, CDCl₃) δ 8.38 (s, 0.2H), 8.33 (s, 0.10H), 8.21 (s, 0.35H),8.09 (s, 0.45H), 7.96 (s, 0.70H), 7.92 (s, 0.30H), 7.86-7.68 (m, 0.70H),7.70-7.43 (m, 0.50H), 7.44-7.09 (m, 3.40H), 6.84 (d, J=7.7 Hz, 0.10H),6.52 (d, J=7.7 Hz, 0.10H), 6.47-6.33 (m, 0.30H), 6.24 (d, J=9.0 Hz,0.10H), 6.08 (d, J=8.8 Hz, 0.70H), 5.31-5.08 (m, 0.35H), 5.08-4.82 (m,0.20H), 4.70 (br d, J=13.7 Hz, 0.50H), 4.26-4.05 (m, 0.90H), 3.99 (br s,0.40H), 3.93-3.80 (m, 0.20H), 3.60 (br s, 0.10H), 3.49 (br d, J=9.6 Hz,0.30H), 3.34-3.12 (m, 0.35H), 3.02-2.70 (m, 0.70H), 2.45 (d, J=7.4 Hz,2.00H), 2.14-1.73 (m, 2.00H), 1.73-1.01 (m, 3.80H), 1.01-0.76 (m,2.00H), 0.71 (d, J=7.0 Hz, 0.20H). The signal corresponding to the NHgroup was not observed.

Example 16(±)-trans-(3-ethoxy-6-methylpyridin-2-yl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogously to Example 12 substituting intermediate A-4 withintermediate A-12. MS (ESI) mass calcd. for C₂₁H₂₅F₃N₄O₂, 422.2. m/zfound 423.2 [M+H]⁺. MP=153.6° C. The product is present as a mixture ofconformers (ratio ca: 70:30) ¹H NMR (300 MHz, DMSO) δ 8.34 (s, 0.3H),7.97 (s, 0.7H), 7.65 (dd, J=8.9, 2.4 Hz, 0.3H), 7.59 (dd, J=8.9, 2.5 Hz,0.7H), 7.39 (d, J=8.6 Hz, 0.3H), 7.27-7.12 (m, 2H), 6.96 (d, J=8.6 Hz,0.7H), 6.75 (d, J=8.9 Hz, 0.3H), 6.69 (d, J=8.9 Hz, 0.7H), 4.85 (q,J=6.9 Hz, 0.3H), 4.48-4.33 (m, 0.7H), 4.11-3.72 (m, 3H), 3.59 (d, J=2.4Hz, 0.7H), 3.22-3.08 (m, 0.3H), 3.07-2.96 (m, 0.3H), 2.96-2.82 (m,0.7H), 2.38 (s, 0.9H), 2.10-1.54 (m, 6.1H), 1.32-1.22 (m, 6H).

Example 17(±)-trans-(5-chloro-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-13. MS (ESI) mass calcd. for C₂₁H₂₀ClF₃N₆O, 464.2. m/zfound 465.1 [M+H]⁺. MP=224.4° C. (Mixture of 4 isomers, undefinedratio). ¹H NMR (300 MHz, DMSO) δ 8.42-8.24 (m, 0.64H), 8.20 (s, 0.12H),8.11 (d, J=3.7 Hz, 1.64H), 8.04 (s, 0.48H), 7.96 (dd, J=8.7, 5.0 Hz,0.41H), 7.85-7.53 (m, 2.49H), 7.53-7.27 (m, 1.57H), 7.24 (s, 0.08H),7.13 (d, J=2.4 Hz, 0.50H), 6.89-6.69 (m, 0.91H), 6.63 (s, 0.08H), 6.12(s, 0.08H), 4.91 (d, J=6.7 Hz, 0.08H), 4.64 (d, J=7.2 Hz, 0.41H), 4.37(d, J=13.0 Hz, 0.63H), 4.11 (s, 0.38H), 4.01 (s, 0.08H), 3.90 (d, J=18.5Hz, 0.24H), 3.68 (dd, J=23.6, 6.5 Hz, 0.76H), 3.21 (d, J=16.3 Hz,0.41H), 3.05-2.91 (m, 0.41H), 2.78 (t, J=11.7 Hz, 0.6H), 2.15-1.46 (m,3.56H), 1.45-1.15 (m, 1.92H), 0.70 (d, J=6.9 Hz, 1.52H).

Example 18(±)-trans-(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-22. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/zfound 446.0 [M+H]⁺. MP=105.7° C. (Mixture of 4 isomers, undefinedratio). ¹H NMR (300 MHz, CDCl₃) δ 8.39-8.22 (m, 1.15H), 8.17 (d, J=8.4Hz, 0.74H), 8.05 (s, 0.33H), 7.92 (s, 1.23H), 7.64-7.51 (m, 0.23H), 7.45(d, J=8.7 Hz, 0.74H), 7.38-7.29 (m, 1.15H), 6.96 (br s, 0.74H), 6.44 (d,J=8.8 Hz, 0.23H), 6.33 (br s, 0.23H), 6.17 (br s, 0.23H), 5.11 (q, J=7.2Hz, 0.23H), 4.65 (br d, J=13.3 Hz, 0.74H), 4.13-3.85 (m, 1.35H), 3.49(br s, 0.47H), 3.42-3.17 (m, 0.47H), 3.11-2.92 (m, 0.74H), 2.82 (s,0.47H), 2.72-2.53 (m, 2.53H), 2.14-1.74 (m, 2.30H), 1.54-1.03 (m,2.53H), 1.00-0.75 (m, 2.17H). The signal corresponding to the NH groupwas not observed.

Example 19(±)-trans-(5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-14. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O₂, 460.2. m/zfound 461.0 [M+H]⁺. MP=170.8° C. (Mixture of 4 isomers, undefinedratio). ¹H NMR (300 MHz, CDCl₃) δ 8.41-8.29 (m, 0.33H), 8.28-8.11 (m,0.55H), 8.05 (s, 0.45H), 7.99 (d, J=9.0 Hz, 0.27H), 7.93 (s, 0.90H),7.90-7.65 (m, 1.00H), 7.59 (dd, J=8.7, 2.2 Hz, 0.33H), 7.54-7.38 (m,0.59H), 7.35 (dd, J=8.8, 2.3 Hz, 0.5H), 7.11-6.94 (m, 1.00H), 6.83 (d,J=2.7 Hz, 0.45H), 6.77 (d, J=2.8 Hz, 0.33H), 6.61 (d, J=2.7 Hz, 0.10H),6.41 (t, J=9.1 Hz, 0.33H), 6.23 (d, J=8.7 Hz, 0.10H), 6.13 (d, J=8.8 Hz,0.50H), 5.99 (br s, 0.27H), 5.26-5.09 (m, 0.37H), 5.07-4.90 (m, 0.20H),4.68 (br d, J=13.5 Hz, 0.60H), 4.25-4.04 (m, 1.06H), 3.98 (s, 0.41H),3.93-3.78 (m, 2.48H), 3.57 (s, 0.25H), 3.54-3.39 (m, 0.72H), 3.22 (td,J=13.4, 3.2 Hz, 0.39H), 2.93 (td, J=13.1, 3.0 Hz, 0.52H), 2.13-1.59 (m,3.30H), 1.52 (d, J=6.9 Hz, 1.51H), 1.42 (d, J=7.1 Hz, 0.78H), 1.37-1.19(m, 0.78H), 1.00-0.62 (m, 0.63H). The signal corresponding to the NHgroup was not observed.

Example 20(±)-trans-(3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-15. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O, 444.2. m/zfound 445.2 [M+H]⁺. MP=153.0. (Mixture of 4 isomers, undefined ratio).¹H NMR (300 MHz, CDCl₃) δ 8.41-8.31 (m, 0.19H), 8.26 (s, 0.56H), 8.07(s, 0.19H), 7.98 (s, 1.09H), 7.90-7.70 (m, 0.76H), 7.64-7.52 (m, 0.19H),7.52-7.33 (m, 2.15H), 7.25-7.09 (m, 0.75H), 6.76 (br d, J=26.3 Hz,0.19H), 6.49 (d, J=8.8 Hz, 0.56H), 6.43 (d, J=9.0 Hz, 0.19H), 6.30 (d,J=8.8 Hz, 0.19H), 5.17 (br d, J=6.9 Hz, 0.19H), 5.00-4.76 (m, 0.19H),4.53 (br d, J=13.4 Hz, 0.56H), 4.20-4.00 (m, 1.09H), 3.94 (br s, 0.19H),3.64-3.55 (m, 0.19H), 3.51 (d, J=5.3 Hz, 0.35H), 3.40 (br d, J=14.7 Hz,0.19H), 3.22 (s, 0.19H), 2.96 (s, 0.19H), 2.86 (td, J=13.2, 3.4 Hz,0.66H), 2.32 (s, 1.75H), 2.28 (s, 0.35H), 2.22 (s, 0.19H), 2.13 (br s,0.19H), 2.09-1.77 (m, 2.15H), 1.77-1.63 (m, 1.74H), 1.51 (d, J=7.0 Hz,2.15H), 1.38 (d, J=7.1 Hz, 0.56H), 1.33-1.21 (m, 0.19H), 1.15 (d, J=6.1Hz, 0.19H), 1.07 (d, J=6.9 Hz, 0.19H), 1.02-0.82 (m, 0.35H). The signalcorresponding to the NH group was not observed.

Example 21(±)-trans-(3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-16. MS (ESI) mass calcd. for C₂₀H₂₀F₃N₂O, 431.2. m/zfound 432.2 [M+H]⁺. MP=79.6° C. (Mixture of 4 isomers, undefined ratio).¹H NMR (300 MHz, CDCl₃) δ 8.64 (dd, J=4.7, 1.3 Hz, 0.3H), 8.57 (d, J=3.6Hz, 0.7H), 8.45 (d, J=8.4 Hz, 0.3H), 8.38 (s, 0.3H), 8.31 (d, J=8.2 Hz,0.7H), 8.24 (s, 0.3H), 8.12 (s, 0.7H), 7.95 (s, 1.0H), 7.59 (d, J=8.4Hz, 0.3H), 7.55-7.34 (m, 1.4H), 6.99 (br d, J=6.9 Hz, 0.7H), 6.43 (d,J=8.9 Hz, 0.3H), 6.32 (br s, 0.7H), 6.10 (br s, 0.3H), 5.15 (br d, J=7.2Hz, 0.3H), 4.66 (br d, J=13.4 Hz, 0.7H), 4.20-3.83 (m, 0.9H), 3.49 (d,J=5.0 Hz, 0.7H), 3.44-3.26 (m, 0.7H), 3.14-2.94 (m, 0.7H), 2.13-1.76 (m,2.7H), 1.75-1.61 (m, 1.20H), 1.48 (dd, J=6.9, 4.2 Hz, 2.5H), 0.99-0.93(m, 0.3H), 0.82-0.76 (m, 0.3H). The signal corresponding to the NH groupwas not observed.

Example 22(±)-trans-(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanonehydrochloride

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-18. MS (ESI) mass calcd. for C₂₂H₂₂F₃N₅O₂, 445.2. m/zfound 446.2 [M+H]⁺. (Mixture of 4 isomers, undefined ratio). ¹H NMR (300MHz, DMSO) δ 8.46 (s, 0.31H), 8.37 (d, J=6.2 Hz, 0.58H), 8.31 (s,0.58H), 8.24-8.02 (m, 0.47H), 7.82 (d, J=7.4 Hz, 0.76H), 7.73-7.44 (m,3.35H), 7.37 (d, J=8.2 Hz, 0.76H), 6.89 (d, J=9.0 Hz, 0.47H), 6.59 (brs, 0.72H), 4.94 (d, J=6.7 Hz, 0.58H), 4.51 (d, J=12.1 Hz, 0.76H), 3.86(d, J=6.8 Hz, 0.66H), 3.76 (br s, 0.58H), 3.30-3.08 (m, 0.66H), 3.00 (t,J=11.7 Hz, 0.76H), 2.63 (s, 1.27H), 2.36 (s, 1.73H), 2.19-1.89 (m,1.73H), 1.88-1.56 (m, 2.27H), 1.41 (d, J=6.6 Hz, 1.27H), 1.21 (d, J=6.5Hz, 1.73H).

Example 23(±)-trans-(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanonehydrochloride

Prepared analogous to Example 12 substituting intermediate A-4 withintermediate A-19. MS (ESI) mass calcd. for C₂₃H₂₃F₃N₆O, 456.2. m/zfound 457.2 [M+H]⁺. (Mixture of 4 isomers, undefined ratio). ¹H NMR (300MHz, DMSO) δ 9.00 (d, J=4.9 Hz, 1.90H), 8.86 (d, J=8.2 Hz, 0.45H),8.45-8.36 (m, 1.00H), 8.53-8.08 (m, 0.67H), 8.21 (s, 0.55H), 7.85 (dd,J=27.3, 8.5 Hz, 1.33H), 7.69 (d, J=8.4 Hz, 0.55H), 7.60 (dt, J=11.9, 4.9Hz, 1.00H), 7.44 (d, J=8.2 Hz, 0.55H), 7.22 (d, J=9.0 Hz, 0.45H), 6.62(br s, 0.55H), 4.77 (br d, J=7.2 Hz, 0.67H), 4.46 (br d, J=13.3 Hz,0.67H), 4.00-3.80 (m, 1.15H), 3.29 (br s, 0.78H), 2.99 (t, J=12.3 Hz,0.78H), 2.81 (s, 1.40H), 2.44 (s, 1.60H), 2.17-1.73 (m, 3.18H), 1.66 (brd, J=12.3 Hz, 0.67H), 1.59-1.25 (m, 3.15H).

Example 24(±)-trans-(5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 14 substituting intermediate A-11 withintermediate A-24. MS (ESI) mass calcd. for C₂₁H₂₀F₄N₆O, 448.2. m/zfound 449.2 [M+H]⁺. MP=168.1° C. The product is present as a mixture ofconformers (ratio ca. 60:40) ¹H NMR (300 MHz, DMSO) δ 8.32 (s, 0.4H),8.08 (s, 1.2H), 8.07 (s, 0.8H), 8.00 (s, 0.6H), 7.94 (dd, J=9.0, 4.9 Hz,0.4H), 7.77-7.56 (m, 2H), 7.54-7.28 (m, 1.6H), 7.10 (td, J=8.5, 2.9 Hz,0.6H), 6.87-6.76 (m, 1.4H), 4.62 (q, J=7.0 Hz, 0.4H), 4.43-4.23 (m,0.6H), 3.79-3.56 (m, 1.6H), 3.23 (d, J=15.9 Hz, 0.4H), 3.08-2.93 (m,0.4H), 2.85-2.68 (m, 0.6H), 2.13-1.29 (m, 4H), 1.25 (d, J=7.0 Hz, 1.2H),0.67 (d, J=6.9 Hz, 1.8H).

Example 25((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanonehydrochloride

Prepared analogous to Example 14 substituting intermediate A-11 withintermediate A-18. MS (ESI) mass calcd. for C₂₂H₂₂F₃N₅O₂, 445.2. m/zfound 446.2 [M+H]⁺. The product is present as a mixture of conformers(ratio ca. 70:30) ¹H NMR (300 MHz, CDCl₃) δ 8.36 (s, 0.3H), 8.29-8.20(m, 1H), 8.14 (d, J=8.2 Hz, 0.7H), 7.81 (s, 1H), 7.65-7.49 (m, 0.6H),7.41 (d, J=7.0 Hz, 0.7H), 7.36 (s, 0.7H), 7.32-7.22 (m, 1H), 7.12 (br s,0.7H), 6.84 (br s, 0.3H), 6.46 (d, J=8.8 Hz, 0.3H), 6.24 (d, J=8.4 Hz,0.7H), 5.20 (q, J=7.8 Hz, 0.3H), 4.82-4.64 (m, 0.7H), 4.12 (d, J=6.3 Hz,0.3H), 4.02 (d, J=5.6 Hz, 0.7H), 3.76 (q, J=6.7 Hz, 0.7H), 3.36-3.14 (m,0.6H), 3.11-2.94 (m, 0.7H), 2.63 (s, 0.9H), 2.60 (s, 2.1H), 2.09-1.52(m, 4H), 1.49 (d, J=7.1 Hz, 0.9H), 1.43 (d, J=6.9 Hz, 2.1H).

Example 26((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 14 substituting intermediate A-11 withintermediate A-21. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/zfound 446.2 [M+H]⁺. MP=116.2° C. The product is present as a mixture ofconformers (ratio ca. 70:30) ¹H NMR (300 MHz, CDCl₃) δ 8.45 (s, 0.3H),8.38 (s, 1.1H), 8.24 (s, 1.1H), 8.15-8.04 (m, 1.1H), 7.93 (s, 1.1H),7.58 (dd, J=8.8, 2.1 Hz, 1.4H), 7.45 (d, J=7.1 Hz, 0.3H), 6.99 (d, J=7.2Hz, 0.7H), 6.43 (d, J=8.8 Hz, 0.3H), 6.31 (d, J=8.4 Hz, 0.7H), 6.15 (d,J=7.7 Hz, 0.3H), 5.13 (q, J=6.7 Hz, 0.3H), 4.64 (d, J=12.6 Hz, 0.7H),4.09-3.85 (m, 1.7H), 3.45-3.21 (m, 0.6H), 3.11-2.92 (m, 0.7H), 2.48 (s,0.9H), 2.44 (s, 2.1H), 2.10-1.72 (m, 3.3H), 1.65-1.58 (m, 0.7H),1.52-1.41 (m, 3H).

Example 27((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 14 substituting intermediate A-11 withintermediate A-20. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/zfound 446.2 [M+H]⁺. MP=111.4° C. The product is present as a mixture ofconformers (ratio ca. 80:20) ¹H NMR (300 MHz, CDCl₃) δ 8.93-8.74 (m,2H), 8.59 (d, J=8.2 Hz, 0.2H), 8.38 (d, J=8.1 Hz, 0.8H), 8.28 (s, 0.2H),8.20 (s, 0.8H), 7.52-7.13 (m, 2.8H), 6.48 (d, J=8.8 Hz, 0.4H), 6.17 (d,J=5.9 Hz, 0.8H), 5.06-4.88 (m, 0.2H), 4.62 (d, J=13.3 Hz, 0.8H), 4.12(d, J=7.0 Hz, 0.2H), 4.04-3.79 (m, 1.8H), 3.32-3.11 (m, 0.2H), 3.08-2.86(m, 0.8H), 2.60 (s, 0.6H), 2.57 (s, 2.4H), 2.10-1.54 (m, 4H), 1.50-1.41(m, 3H).

Example 28(±)-trans-(3-fluoro-2-methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 42 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid and 5-bromo-2-fluoropyridine with2-chloro-5-(trifluoromethyl)pyridine. MS (ESI) mass calcd. forC₂₀H₂₁F₄N₃O₂, 411.2. m/z found 412.2 [M+H]⁺. 1H NMR (CDCl3): 8.35-8.05(m, 1H), 7.76-7.39 (m, 1H), 7.17-5.95 (m, 4H), 5.26-4.63 (m, 1H),4.29-2.75 (m, 6H), 2.17-1.56 (m, 4H), 1.48-1.23 (m, 3H).

Example 29(±)-trans-(2-ethoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 42 substituting intermediate A-4 with2-ethoxybenzoic acid and 5-bromo-2-fluoropyridine with2-chloro-5-(trifluoromethyl)pyridine. MS (ESI) mass calcd. forC₂₁H₂₄F₃N₃O₂, 407.2. m/z found 408.2 [M+H]⁺. 1H NMR (CDCl3): 8.32-8.04(m, 1H), 7.85-7.27 (m, 2H), 7.21-6.23 (m, 4H), 5.25-2.83 (m, 5H),2.07-1.24 (m, 11H).

Example 30(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Step A:(±)-trans-tert-butyl-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylate

To intermediate B-3 (300 mg, 1.4 mmol) in n-BuOH (5 mL) was added2,5-dichloropyrimidine (210 mg, 1.4 mmol) and DIPEA (480 μL, 2.8 mmol).The reaction was then heated at 120° C. overnight. The reaction wasallowed to cool to rt, diluted with H₂O and extracted with EtOAc. Thecombined organics were dried (MgSO₄). Silica gel chromatography (EtOAcin heptane) gave the title compound (237 mg, 43%). MS (ESI) mass calcd.for C₁₅H₂₃ClN₄O₂, 326.2. m/z found 327.0 [M+H]⁺.

Step B. trans-(±)-5-chloro-N-(2-methylpiperidin-3-yl)pyrimidin-2-amine

Prepared analogous to Example 1 substituting(±)-tert-butyl-3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylatewith the title compound from Step A.

Step C.(±)-trans-tert-butyl-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylate

Prepared analogous to Example 12 substitutingtrans-(±)-2-methylpiperidin-3-yl)-5-(trifluoromethyl)pyridin-2-aminewith the title compound from Step B. MS (ESI) mass calcd. forC₁₉H₂₀ClN₇O, 397.2. m/z found 398.2 [M+H]⁺. MP=200.5° C. The product ispresent as a mixture of conformers (ratio ca. 60:40) ¹H NMR (300 MHz,CDCl₃) δ 8.31-7.77 (m, 5H), 7.62-7.24 (m, 3.4H), 6.60 (d, J=8.4 Hz,0.6H), 5.25 (q, J=6.9 Hz, 0.6H), 4.80-4.68 (m, 0.4H), 4.26-4.03 (m, 1H),3.93 (d, J=7.4 Hz, 0.4H), 3.51 (dd, J=13.7, 3.9 Hz, 0.6H), 3.25 (td,J=13.4, 3.3 Hz, 0.6H), 3.08-2.84 (m, 0.4H), 1.96-1.59 (m, 4H), 1.50 (d,J=6.9 Hz, 1.2H), 1.44 (d, J=7.1 Hz, 1.8H).

Example 31(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-methylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 30 substituting 2,5-dichloropyrimidinewith 2-chloro-5-methylpyrimidine and n-BuOH with DMSO. MS (ESI) masscalcd. for C₂₀H₂₃N₂O, 377.2. m/z found 378.2 [M+H]⁺. MP=153.0° C.

Example 32(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Step A:trans-(±)-tert-butyl-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidine-1-carboxylate

To 5-chloro-2-iodopyridine (246 mg, 1 mmol) in THF 10 mL) was addedPd₂(dba)₃ (30 mg, 0.03 mmol), Xantphos (38 mg, 0.07 mmol) and sodiumtert-butoxide (180 mg, 1.9 mmol). Then N₂ was bubbled through thesolution for 10 min and intermediate B-3 (200 mg, 0.9 mmol) was added.The reaction was then heated at 90° C. overnight, cooled to rt andsaturated NaHCO₃ (aq) was added followed by extraction with EtOAc. Thecombined organics were dried and purified via silica gel chromatography(EtOAc in heptane) to give the title compound (98 mg, 32%). MS (ESI)mass calcd. for C₁₆H₂₄ClN₃O₂, 325.2. m/z found 326.0 [M+H]⁺.

Step B: trans-(±)-5-chloro-N-(2-methylpiperidin-3-yl)pyridin-2-amine

Prepared analogous to Example 1 substituting(±)-tert-butyl-3-(4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylatewith the title compound from Step A.

Step C:(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 30 substitutingtrans-(±)-2-methylpiperidin-3-yl)-5-(trifluoromethyl)pyridin-2-aminewith the title compound from Step B. MS (ESI) mass calcd. forC₂₀H₂₁ClN₆O, 396.2. m/z found 397.0 [M+H]⁺. MP=128.0° C. The product ispresent as a mixture of conformers (12:13:35:40). ¹H NMR (300 MHz, DMSO)8.25 (d, J=12.3 Hz, 0.37H), 8.07 (s, 1.48H), 7.98 (d, J=2.6 Hz, 0.25H),7.97-7.86 (m, 0.48H), 7.86-7.77 (m, 0.25H), 7.73 (d, J=8.1 Hz, 0.48H),7.70-7.40 (m, 2.76H), 7.34 (t, J=7.1 Hz, 0.75H), 7.26 (d, J=11.5 Hz,0.13H), 7.07 (d, J=6.5 Hz, 0.40H), 6.93 (d, J=6.8 Hz, 0.53H), 6.87 (d,J=7.7 Hz, 0.40H), 6.78 (t, J=7.1 Hz, 0.48H), 6.72 (d, J=9.0 Hz, 0.88H),6.62 (d, J=9.1 Hz, 0.12H), 6.08 (d, J=9.0 Hz, 0.12H), 6.03 (d, J=7.9 Hz,0.12H), 4.95-4.82 (m, 0.13H), 4.66 (q, J=7.5 Hz, 0.37H), 4.38 (br d,J=13.9 Hz, 0.41H), 4.01-3.89 (m, 0.25H), 3.89-3.77 (m, 0.40H), 3.70 (d,J=7.4 Hz, 0.48H), 3.19 (br d, J=14.4 Hz, 0.48H), 2.92 (br t, J=11.7 Hz,0.48H), 2.84-2.65 (m, 1H), 2.13-1.47 (m, 4H), 1.43 (d, J=6.9 Hz, 0.36H),1.31 (d, J=7.0 Hz, 0.39H), 1.24 (d, J=7.0 Hz, 1.05H), 0.77 (d, J=7.0 Hz,1.20H).

Example 33(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((6-(trifluoromethyl)pyridazin-3-yl)amino)piperidin-1-yl)methanonehydrochloride

Prepared analogous to Example 31 substituting2-chloro-5-methylpyrimidine with 3-chloro-6-(trifluoromethyl)pyridazine.MS (ESI) mass calcd. for C₂₀H₂₀F₃N₇O, 431.2. m/z found 432.0 [M+H]+.MP=167.0° C. (Mixture of 4 isomers, undefined ratio). ¹H NMR (300 MHz,DMSO) δ 8.33-7.98 (m, J=25.2, 1.88H), 7.91 (d, J=8.0 Hz, 0.43H),7.85-7.31 (m, 4.06H), 7.31-7.03 (m, 1.49H), 6.97 (d, J=6.5 Hz, 0.50H),6.72 (t, J=7.2 Hz, 0.50H), 6.50 (s, 0.14H), 5.14-4.93 (m, 0.14H), 4.77(q, J=7.0 Hz, 0.43H), 4.52-4.30 (m, 0.70H), 4.26-4.05 (m, 0.58H),3.38-3.10 (m, 0.83H), 3.06-2.87 (m, 0.63H), 2.79 (t, J=11.8 Hz, 0.69H),2.20-1.50 (m, 3.64H), 1.50-1.17 (m, 2.07H), 0.73 (d, J=6.9 Hz, 1.29H).

Example 34(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 32 substituting 5-chloro-2-iodopyridinewith 5-fluoro-2-iodopyridine. MS (ESI) mass calcd. for C₂₀H₂₁FN₆O,380.2. m/z found 381.2 [M+H]⁺. MP=189.4° C. (Mixture of 4 isomers,undefined ratio). ¹H NMR (300 MHz, DMSO) δ 8.25 (d, J=11.1 Hz, 0.44H),8.15-7.99 (m, 1.77H), 7.90 (d, J=8.1 Hz, 0.52H), 7.84-7.42 (m, 4.24H),7.35 (t, J=7.8 Hz, 0.65H), 7.12 (d, J=7.7 Hz, 0.71H), 7.06-6.91 (m,0.51H), 6.91-6.77 (m, 0.78H), 6.76-6.67 (m, 0.16H), 6.21-6.07 (m,0.22H), 4.93-4.76 (m, 0.35H), 4.66 (q, J=6.9 Hz, 0.61H), 4.38 (d, J=12.7Hz, 0.83H), 3.30-3.11 (m, 0.85H), 3.03-2.85 (m, 0.86H), 2.84-2.71 (m,0.5H), 2.12-1.47 (m, 3.52H), 1.48-1.14 (m, 2.50H), 0.79 (d, J=6.9 Hz,0.98H).

Example 35(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 30 substituting 2,5-dichloropyrimidinewith 2-chloro-5-(trifluoromethyl)pyrimidine. MS (ESI) mass calcd. forC₂₀H₂₀F₃N₇O, 431.2. m/z found 432.2 [M+H]₊. MP=204.4° C. (Mixture of 4isomers, undefined ratio). ¹H NMR (300 MHz, CDCl₃) δ 8.60-8.45 (m,1.37H), 8.45-8.23 (m, 1.95H), 8.23-8.11 (m, 0.63H), 8.12-8.00 (m,0.58H), 7.96 (d, J=7.3 Hz, 0.27H), 7.88 (d, J=8.3 Hz, 0.09H), 7.82 (d,J=6.5 Hz, 0.27H), 7.56 (td, J=7.9, 1.5 Hz, 0.89H), 7.51-7.33 (m, 1.45H),7.30 (d, J=1.4 Hz, 0.78H), 7.07 (dd, J=7.7, 1.3 Hz, 0.08H), 6.97 (t,J=10.4 Hz, 0.56H), 6.81 (t, J=7.5 Hz, 0.08H), 5.88 (d, J=7.3 Hz, 0.08H),5.72 (d, J=6.2 Hz, 0.08H), 5.28 (q, J=6.9 Hz, 0.48H), 5.05 (d, J=7.1 Hz,0.08H), 4.76 (d, J=13.6 Hz, 0.38H), 4.40-4.24 (m, 0.48H), 4.14 (q, J=7.1Hz, 0.38H), 4.04 (d, J=7.5 Hz, 0.22H), 3.97-3.83 (m, 0.08H), 3.68 (s,0.08H), 3.53 (dd, J=15.5, 4.7 Hz, 0.7H), 3.27 (td, J=13.4, 3.3 Hz,0.58H), 3.14-2.76 (m, 0.38H), 2.16-1.21 (m, 6.56H), 1.15 (d, J=6.1 Hz,0.08H), 1.05-0.93 (m, 0.08H), 0.93-0.82 (m, 0.12H), 0.73 (d, J=7.0 Hz,0.16H).

Example 36(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(pyridin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 32 substituting 5-chloro-2-iodopyridinewith 2-iodopyridine. MS (ESI) mass calcd. for C₂₀H₂₂N₆O, 362.2. m/zfound 363.2 [M+H]⁺. MP=277.1° C. (Mixture of 4 isomers, undefinedratio). ¹H NMR (300 MHz, DMSO) δ 8.99-8.49 (m, 0.83H), 8.40 (d, J=5.3Hz, 0.15H), 8.22 (br s, 0.32H), 8.18-7.73 (m, 4.79H), 7.70-7.43 (m,1.78H), 7.42-7.03 (m, 1.68H), 7.03-6.77 (m, 1.25H), 6.77-6.67 (m,0.10H), 6.67-6.51 (m, 0.10H), 4.92-4.77 (m, 0.15H), 4.68 (q, J=6.8 Hz,0.60H), 4.43 (br d, J=13.9 Hz, 0.42H), 4.06 (br s, 0.60H), 3.92 (br s,0.32H), 3.86-3.67 (m, 0.60H), 3.24 (br s, 0.29H), 2.99 (t, J=11.9 Hz,0.60H), 2.83 (t, J=11.5 Hz, 0.42H), 2.24-1.54 (m, 3.04H), 1.54-1.12 (m,3.13H), 0.88 (d, J=6.6 Hz, 0.83H).

Example 37(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-methylpyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 32 substituting 5-chloro-2-iodopyridinewith 2-chloro-5-methylpyridine. MS (ESI) mass calcd. for C₂₁H₂₄N₆O,376.2. m/z found 377.2 [M+H]⁺. MP=148.0° C. (Mixture of 4 isomers,undefined ratio). ¹H NMR (300 MHz, DMSO) δ 8.66 (d, J=8.0 Hz, 0.64H),8.57 (br s, 0.34H), 8.25 (d, J=8.1 Hz, 0.41H), 8.14 (d, J=9.9 Hz,1.66H), 8.05-7.76 (m, 3.39H), 7.76-7.52 (m, 1.66H), 7.45 (t, J=8.2 Hz,0.34H), 7.35 (d, J=9.7 Hz, 0.64H), 7.28 (d, J=7.1 Hz, 0.34H), 7.23-7.13(m, 0.16H), 6.99 (t, J=7.5 Hz, 0.34H), 6.60 (br s, 0.08H), 4.86 (br s,0.16H), 4.72 (q, J=6.9 Hz, 0.64H), 4.47 (br d, J=12.9 Hz, 0.41H), 4.07(br s, 0.64H), 3.95 (br s, 0.34H), 3.76 (br s, 0.64H), 3.27 (br s,0.11H), 3.01 (dd, J=21.0, 8.4 Hz, 0.64H), 2.92-2.81 (m, 0.34H), 2.74 (s,0.41H), 2.72-2.67 (m, 0.08H), 2.25 (s, 2.59H), 2.19-1.57 (m, 3.30H),1.56-1.40 (m, 1.14H), 1.37 (d, J=7.0 Hz, 1.57H), 1.29 (s, 0.16H), 0.90(d, J=6.8 Hz, 0.83H).

Example 38(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 31 substituting2-chloro-5-methylpyrimidine with 2-chloro-5-(trifluoromethyl)pyrazineand DIPEA with K₂CO₃. MS (ESI) mass calcd. for C₂₀H₂₀F₃N₂O, 431.2. m/zfound 432.2 [M+H]⁺. ¹H NMR (300 MHz, DMSO) δ 8.42 (br s, 0.5H),8.27-8.17 (m, 1.09H), 8.15 (s, 0.26H), 8.10 (s, 1.31H), 8.08 (s, 0.62H),8.06-7.99 (m, 0.62H), 7.99-7.91 (m, 0.62H), 7.90 (s, 0.26H), 7.80-7.39(m, 2.14H), 7.27 (t, J=7.1 Hz, 0.50H), 7.11 (s, 0.09H), 7.07 (d, J=7.7Hz, 0.45H), 6.94 (s, 0.09H), 6.66 (t, J=7.6 Hz, 0.45H), 4.72 (q, J=6.6Hz, 0.50H), 4.41 (br d, J=12.7 Hz, 0.70H), 4.04 (br s, 0.50H), 3.98-3.90(m, 0.45H), 3.31-3.27 (m, 0.28H), 3.18 (br s, 0.62H), 3.03-2.91 (m,0.50H), 2.84-2.76 (m, 0.45H), 2.31-2.25 (m, 0.26H), 2.15-1.84 (m,1.81H), 1.84-1.67 (m, 1.09H), 1.67-1.49 (m, 1.09H), 1.39 (d, J=7.2 Hz,0.62H), 1.32 (s, 0.09H), 1.27 (d, J=7.0 Hz, 0.95H), 0.66 (d, J=6.9 Hz,1.09H).

Example 39(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinazolin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 30 substituting 2,5-dichloropyrimidinewith 2-chloroquinazoline. MS (ESI) mass calcd. for C₂₃H₂₃N₇O, 413.2. m/zfound 414.2 [M+H]⁺. (Mixture of 4 isomers, undefined ratio). ¹H NMR (300MHz, CDCl₃) δ 9.02 (s, 0.44H), 8.83 (s, 0.15H), 8.35 (s, 0.61H), 8.14(d, J=8.1 Hz, 0.44H), 8.08 (s, 0.28H), 8.00 (d, J=7.6 Hz, 0.33H), 7.91(d, J=7.9 Hz, 0.33H), 7.86-7.10 (m, 7.45H), 7.10-6.94 (m, 0.23H), 6.82(br s, 0.15H), 6.64 (d, J=8.0 Hz, 0.44H), 6.30 (br s, 0.15H), 5.77 (brs, 0.15H), 5.54 (br s, 0.15H), 5.29 (br d, J=5.5 Hz, 0.61H), 5.18-5.03(m, 0.15H), 4.84-4.61 (m, 0.33H), 4.41 (d, J=7.9 Hz, 0.44H), 4.30 (br s,0.15H), 4.25-3.97 (m, 0.61H), 3.91-3.68 (m, 0.15H), 3.50 (d, J=10.1 Hz,0.44H), 3.25 (t, J=11.9 Hz, 0.49H), 3.09-2.69 (m, 0.39H), 2.39-1.81 (m,1.13H), 1.81-1.56 (m, 1.30H), 1.52 (d, J=6.8 Hz, 0.80H), 1.44 (dd,J=13.1, 6.7 Hz, 1.94H), 1.02-0.62 (m, 1.83H). The signal correspondingto the NH group was not observed.

Example 40(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-fluoropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 31 substituting2-chloro-5-methylpyrimidine with 2-chloro-5-fluoropyrimidine. MS (ESI)mass calcd. for C₁₉H₂₀FN₇O, 381.2. m/z found 382.1 [M+H]⁺. (Mixture of 4isomers, undefined ratio). ¹H NMR (300 MHz, DMSO) δ 8.48 (s, 0.47H),8.39 (d, J=7.6 Hz, 1.01H), 8.24 (s, 0.61H), 8.17 (s, 0.47H), 8.07 (d,J=3.7 Hz, 1.01H), 8.02 (d, J=8.4 Hz, 0.37H), 7.88 (d, J=7.0 Hz, 0.47H),7.73 (d, J=7.8 Hz, 0.58H), 7.69-7.28 (m, 2.90H), 7.00 (d, J=6.4 Hz,0.37H), 6.83 (t, J=7.1 Hz, 0.37H), 6.42 (d, J=8.5 Hz, 0.37H), 4.94 (dd,J=13.8, 7.1 Hz, 0.37H), 4.68 (dd, J=13.4, 6.2 Hz, 0.37H), 4.44 (d,J=12.4 Hz, 0.20H), 4.34 (d, J=11.9 Hz, 0.37H), 4.04 (d, J=8.5 Hz,0.37H), 3.94 (t, J=10.0 Hz, 0.37H), 3.77 (dd, J=14.2, 7.0 Hz, 0.37H),3.47-3.38 (m, 0.47H), 3.20 (d, J=13.7 Hz, 0.37H), 3.01-2.82 (m, 0.37H),2.82-2.67 (m, 0.37H), 2.12-1.79 (m, 1.74H), 1.76-1.47 (m, 1.55H), 1.44(d, J=6.9 Hz, 0.52H), 1.35 (d, J=7.2 Hz, 0.86H), 1.33-1.26 (m, 0.61H),1.23 (d, J=7.0 Hz, 0.86H), 0.77 (d, J=7.0 Hz, 0.86H).

Example 41(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)methanone

Step A: (±)-tert-butyl3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidine-1-carboxylate

To intermediate B-3 (1.4 g, 6.6 mmol) in 1,4-dioxane (20 mL) was addedDIPEA (2.3 mL, 13.2 mmol) and 2-(methylsulfinyl)benzo[d]oxazole (2.4 g,13.2 mmol). After heating at 80° C. for 4 h, the mixture was cooled tort and saturated NaHCO3 (aq) was added. The aqueous layer was extractedwith EtOAc (3×). The combined organics were dried (MgSO₄). Purificationvia silica gel chromatography (0-50% EtOAc in hexanes) gave the titlecompound (1.7 g, 77%).

Step B: (±)-N-(-2-methylpiperidin-3-yl)benzo[d]oxazol-2-amine

Prepared analogous to Example 1 substituting(±)-tert-butyl-3-((4,6-dimethylpyrimidin-2-yl)amino)-2-methylpiperidine-1-carboxylatewith the title compound from step A.

Step C:(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 30 substitutingtrans-(±)-5-chloro-N-(2-methylpiperidin-3-yl)pyrimidin-2-amine with thetitle compound from step B. MS (ESI) mass calcd. for C₂₂H₂₂N₆O₂, 402.2.m/z found 403.2 [M+H]⁺. (Mixture of 4 isomers, undefined ratio). ¹H NMR(300 MHz, DMSO) δ 8.03 (dd, J=12.9, 10.4 Hz, 0.82H), 7.91 (d, J=6.3 Hz,0.36H), 7.84 (d, J=2.8 Hz, 1.27H), 7.81-7.70 (m, 0.36H), 7.66 (d, J=7.8Hz, 0.46H), 7.59-7.47 (m, 0.82H), 7.47-7.23 (m, 1.79H), 7.24-7.08 (m,1.27H), 7.08-6.95 (m, 0.61H), 6.95-6.65 (m, 2.88H), 6.16 (t, J=7.8 Hz,0.36H), 4.90-4.76 (m, 0.36H), 4.57 (q, J=7.1 Hz, 0.46H), 4.25-4.06 (m,0.46H), 3.91-3.78 (m, 0.36H), 3.75-3.56 (m, 0.82H), 3.56-3.44 (m,0.36H), 3.32-3.19 (m, 0.36H), 2.97 (br d, J=14.4 Hz, 0.36H), 2.82-2.63(m, 0.46H), 2.06-2.00 (m, 0.18H), 1.91-1.18 (m, 4.56H), 1.18-1.08 (m,0.82H), 0.80 (d, J=6.1 Hz, 0.36H), 0.58 (d, J=6.9 Hz, 1.08H).

Example 42(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Step A: (±)-trans-tert-butyl3-((5-bromopyridin-2-yl)amino)-2-methylpiperidine-1-carboxylate

Intermediate B-3 (1.6 g, 7.5 mmol), 5-bromo-2-fluoropyridine (2.9 g, 15mmol) and K₂CO₃ (4.1 g, 30 mmol) were heated in NMP (50 mL) at 100° C.for 8 h. The reaction was allowed to cool to rt then concentrated andpurified via silica gel chromatography (50% EtOAc in petroleum ethers).

Step B: (±)-trans-5-bromo-N-(2-methylpiperidin-3-yl)pyridin-2-amine

To the title compound from step A in DCM (10 mL) was added TFA (1 mL).After 1 h, the reaction was concentrated to give the title compound thatwas used without further purification.

Step C:(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

The title compound from Step B (50 mg), DIPEA (1 mL), intermediate A-4(100 mg) and HATU (200 mg) were stirred in THF at rt for 3 h. Uponcompletion of the reaction, purification was performed using preparativeHPLC to give the title compound (20 mg, 55% yield). MS (ESI) mass calcd.for C₂₀H₂₁BrN₆O, 440.2. m/z found 441.1 [M+H]⁺. 1H NMR (CDCl3):8.11-7.26 (m, 8H), 7.11-5.91 (m, 1H), 5.19-4.55 (m, 1H), 4.26-2.68 (m,3H), 2.11-0.71 (m, 7H).

Example 43(±)-trans-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2-methoxyphenyl)methanone

Prepared analogous to Example 42 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₁₉H₂₁BrFN₃O₂,421.2. m/z found 422.1 [M+H]⁺. 1H NMR (CDCl₃): 8.03-7.50 (m, 2H),7.33-6.09 (m, 4H), 5.24-4.61 (m, 1H), 4.02-3.33 (m, 5H), 3.30-2.84 (m,1H), 2.24-1.16 (m, 7H).

Example 44(±)-trans-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(2-ethoxyphenyl)methanone

Prepared analogous to Example 42 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₀H₂₄BrN₃O₂, 417.2. m/zfound 418.1 [M+H]⁺. 1H NMR (CDCl3): 7.93-6.19 (m, 7H), 5.20-4.52 (m,1H), 4.32-3.87 (m, 2H), 3.81-2.83 (m, 3H), 2.22-1.76 (m, 3H), 1.52-1.21(m, 7H).

Example 45(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 42 substituting 5-bromo-2-fluoropyridinewith 2,5-dichlorobenzo[d]oxazole. MS (ESI) mass calcd. for C₂₂H₂₁ClN₆O₂,436.2. m/z found 437.2 [M+H]⁺. 1H NMR (CDCl3): 11.55 (s, 1H), 8.14-7.71(m, 4H), 7.60-7.28 (m, 4H), 7.23-6.98 (m, 1H), 5.42-4.67 (m, 1H),4.27-3.94 (m, 1H), 3.50-3.45 (m, 1H), 3.07-2.94 (m, 1H), 2.18-1.80 (m,3H), 1.7-1.39 (m, 4H).

Example 46(±)-trans-(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(2-ethoxyphenyl)methanone

Prepared analogous to Example 45 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₂H₂₄ClN₃O₃, 413.2. m/zfound 414.2 [M+H]⁺. 1H NMR (CDCl3): 7.47-6.19 (m, 7H), 5.85-4.57 (m,1H), 4.57-2.68 (m, 5H), 2.25-0.99 (m, 10H).

Example 47(±)-trans-(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2-methoxyphenyl)methanone

Prepared analogous to Example 45 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₁H₂₁ClFN₃O₃,417.2. m/z found 418.1 [M+H]⁺. 1H NMR (CDCl3): 7.37-7.25 (m, 1H),7.23-6.52 (m, 5H), 5.26-4.62 (m, 1H), 4.24-3.85 (m, 3H), 3.83-3.60 (m,1H), 3.41-3.09 (m, 1H), 2.97-2.88 (m, 1H), 2.32-1.57 (m, 4H), 1.56-1.05(m, 3H).

Example 48(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 42 substituting 5-bromo-2-fluoropyridinewith 2-chloroquinoxaline. MS (ESI) mass calcd. for C₂₃H₂₃N₇O, 413.2. m/zfound 414.2 [M+H]⁺. 1H NMR (CDCl3): 8.40-7.95 (m, 2H), 7.95-7.70 (m,3H), 7.70-7.26 (m, 6H), 5.36-4.56 (m, 1H), 4.42-2.77 (m, 3H), 2.16-0.68(m, 7H).

Example 49(±)-trans-(2-ethoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 48 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₃H₂₆N₄O₂, 390.2. m/zfound 391.2 [M+H]⁺. 1H NMR (CDCl3): 8.72-8.36 (m, 1H), 7.98-7.29 (m,5H), 7.24-6.57 (m, 3H), 5.27-2.90 (m, 6H), 2.13-1.76 (m, 3H), 1.62-1.30(m, 7H).

Example 50(±)-trans-(3-fluoro-2-methoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 48 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₂H₂₃FN₄O₂,394.2. m/z found 395.2 [M+H]⁺. 1H NMR (CDCl3): 8.75-8.26 (m, 1H), 7.96(d, J=8.5 Hz, 1H), 7.84 (t, J=9.2 Hz, 1H), 7.71 (t, J=7.6 Hz, 1H), 7.55(t, J=7.7 Hz, 1H), 7.37-6.08 (m, 3H), 5.32-4.60 (m, 1H), 4.31-3.80 (m,4H), 3.77-2.91 (m, 2H), 2.29-1.51 (m, 4H), 1.51-1.29 (m, 3H).

Example 51(±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 42 substituting 5-bromo-2-fluoropyridinewith 2-chloro-4-phenylpyrimidine. MS (ESI) mass calcd. for C₂₅H₂₅N₇O,439.2. m/z found 440.2 [M+H]⁺.

Example 52(±)-trans-(3-fluoro-2-methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 51 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₄H₂₅FN₄O₂,420.2. m/z found 421.2 [M+H]⁺. 1H NMR (CDCl3): 10.31-9.49 (m, 1H),8.18-8.09 (m, 2H), 7.81-6.43 (m, 8H), 5.57-4.67 (m, 1H), 4.60-4.13 (m,1H), 4.13-2.77 (m, 5H), 2.38-1.88 (m, 3H), 1.88-1.09 (m, 4H).

Example 53(±)-trans-(2-ethoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 51 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₅H₂₈N₄O₂, 416.2. m/zfound 417.2 [M+H]⁺. 1H NMR (CDCl3): 10.37-9.49 (m, 1H), 8.29-6.29 (m,11H), 5.58-4.61 (m, 1H), 4.61-4.25 (m, 1H), 4.25-2.84 (m, 4H), 2.22-1.89(m, 3H), 1.80-1.07 (m, 7H).

Example 54(±)-cis-(3-fluoro-2-methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 28 substituting intermediate B-3 forintermediate B-6. MS (ESI) mass calcd. for C₂₀H₂₁F₄N₃O₂, 411.2. m/zfound 412.1 [M+H]⁺. 1H NMR (CDCl3): 8.38-7.38 (m, 2H), 7.23-5.71 (m,4H), 5.68-3.74 (m, 6H), 3.35-2.78 (m, 1H), 2.03-1.39 (m, 4H), 1.36-1.02(m, 3H).

Example 55(±)-cis-(2-ethoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 54 substituting 3-fluoro-2-methoxybenzoicacid with 2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₁H₂₄F₃N₃O₂,407.2. m/z found 408.1 [M+H]⁺. 1H NMR (CDCl3): 8.37-7.27 (m, 3H),7.25-6.18 (m, 4H), 6.01-3.98 (m, 4H), 3.97-2.68 (m, 2H), 1.95-1.65 (m,3H), 1.59-1.32 (m, 4H), 1.29-0.98 (m, 3H).

Example 56(±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 54 substituting 3-fluoro-2-methoxybenzoicacid with intermediate A-4. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O, 430.2.m/z found 431.2 [M+H]⁺. 1H NMR (CDCl3): 8.14-7.72 (m, 4H), 7.64-7.30 (m,3H), 7.24-6.41 (m, 2H), 5.32-2.70 (m, 4H), 2.06-1.35 (m, 4H), 1.33-1.16(m, 3H).

Example 57(±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 42 substituting Intermediate B-3 withIntermediate B-6. MS (ESI) mass calcd. for C₂₀H₂₁BrN₆O, 440.2. m/z found441.1 [M+H]⁺. 1H NMR (CDCl3): 9.69 (brs, 1H), 8.10-7.67 (m, 4H),7.65-7.38 (m, 3H), 7.36-6.01 (m, 2H), 5.35-4.43 (m, 1H), 4.03-2.60 (m,3H), 2.03-1.77 (m, 2H), 1.76-0.76 (m, 5H).

Example 58(±)-cis-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2-methoxyphenyl)methanone

Prepared analogous to Example 57 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₁₉H₂₁BrFN₃O₂,421.2. m/z found 421.9 [M+H]⁺. 1H NMR (CDCl3): 8.10-7.27 (m, 2H),7.23-6.72 (m, 3H), 6.71-5.94 (m, 1H), 5.41-4.52 (m, 1H), 4.19-3.53 (m,4H), 3.35-2.77 (m, 3H), 2.08-0.99 (m, 7H).

Example 59(±)-cis-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(2-ethoxyphenyl)methanone

Prepared analogous to Example 57 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₀H₂₄BrN₃O₂, 417.2. m/zfound 418.0 [M+H]⁺. 1H NMR (CDCl3): 8.01-7.29 (m, 3H), 7.24-6.81 (m,3H), 6.51-5.76 (m, 1H), 5.36-4.64 (m, 1H), 4.35-3.78 (m, 3H), 3.77-2.76(m, 2H), 2.11-0.83 (m, 10H).

Example 60(±)-cis-((2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 45 substituting intermediate B-3 withintermediate B-6. MS (ESI) mass calcd. for C₂₂H₂₁ClN₆O₂, 436.2. m/zfound 437.1 [M+H]⁺. 1H NMR (CDCl3): 11.37-10.92 (m, 1H), 8.21-7.97 (m,1H), 7.90-7.88 (m, 1H), 7.81-7.80 (m, 1H), 7.61-7.27 (m, 5H), 7.24-7.10(m, 1H), 5.47-4.04 (m, 1H), 4.01-3.46 (m, 1H), 3.41-2.74 (m, 2H),2.06-0.96 (m, 7H).

Example 61(±)-cis-(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(2-ethoxyphenyl)methanone

Prepared analogous to Example 60 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₂H₂₄ClN₃O₃, 413.2. m/zfound 414.0 [M+H]⁺. 1H NMR (CDCl3): 7.42-7.27 (m, 3H), 7.21-6.84 (m,4H), 5.39-4.70 (m, 1H), 4.27-3.68 (m, 4H), 3.41-2.74 (m, 1H), 2.07-1.61(m, 3H), 1.60-1.04 (m, 7H).

Example 62(±)-cis-(3-((5-chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2-methoxyphenyl)methanone

Prepared analogous to Example 60 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₁H₂₁ClFN₃O₃,417.2. m/z found 417.9 [M+H]⁺. 1H NMR (CDCl3): 7.35-6.88 (m, 6H),5.54-3.57 (m, 5H), 3.39-2.72 (m, 3H), 2.12-1.37 (m, 4H), 1.33-1.00 (m,3H).

Example 63(±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 48 substituting intermediate B-3 withintermediate B-6. MS (ESI) mass calcd. for C₂₃H₂₃N₇O, 413.2. m/z found414.2 [M+H]⁺. 1H NMR (CDCl3): 11.03 (s, 1H), 8.97-8.37 (m, 1H),8.14-7.28 (m, 10H), 5.41-3.90 (m, 2H), 3.74-2.57 (m, 2H), 2.20-1.82 (m,2H), 1.80-0.85 (m, 5H).

Example 64(±)-cis-(3-fluoro-2-methoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 63 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₂H₂₃FN₄O₂,394.2. m/z found 395.2 [M+H]⁺. 1H NMR (CDCl3): 8.96-8.11 (m, 1H),8.08-7.64 (m, 3H), 7.55 (s, 1H), 7.22-6.85 (m, 3H), 5.45-4.59 (m, 1H),4.41-2.79 (m, 6H), 2.30-1.06 (m, 7H).

Example 65(±)-cis-(2-ethoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone

Prepared analogous to Example 63 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₃H₂₆N₄O₂, 390.2. m/zfound 391.2 [M+H]⁺. 1H NMR (CDCl3): 8.96-8.35 (m, 1H), 8.01-7.29 (m,5H), 7.25-6.59 (m, 3H), 5.43-5.16 (m, 1H), 4.81-2.64 (m, 5H), 2.26-1.71(m, 3H), 1.71-1.12 (m, 7H).

Example 66(±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 51 substituting intermediate B-3 withintermediate B-6. MS (ESI) mass calcd. for C₂₅H₂₅N₇O, 439.2. m/z found440.2 [M+H]⁺. 1H NMR (CDCl3): 9.88-9.51 (m, 1H), 8.35-7.93 (m, 3H),7.92-7.27 (m, 8H), 7.24-5.46 (m, 2H), 4.98-3.88 (m, 2H), 3.49-2.73 (m,2H), 2.09-0.84 (m, 7H).

Example 67(±)-cis-(3-fluoro-2-methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 66 substituting intermediate A-4 with3-fluoro-2-methoxybenzoic acid. MS (ESI) mass calcd. for C₂₄H₂₅FN₄O₂,420.2. m/z found 421.2 [M+H]⁺. 1H NMR (CDCl3): 10.18-9.91 (m, 1H),8.42-7.48 (m, 6H), 7.24-5.53 (m, 4H), 4.76-3.81 (m, 5H), 3.79-2.77 (m,2H), 2.13-1.41 (m, 4H), 1.38-0.97 (m, 3H).

Example 68(±)-cis-(2-ethoxyphenyl)(2-methyl-3-(4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 66 substituting intermediate A-4 with2-ethoxybenzoic acid. MS (ESI) mass calcd. for C₂₅H₂₈N₄O₂, 416.2. m/zfound 417.2 [M+H]⁺. 1H NMR (CDCl3): 9.34 (s, 1H), 8.32-7.89 (m, 3H),7.74-7.46 (m, 3H), 7.37-7.31 (m, 1H), 7.23-6.73 (m, 3H), 6.29-5.49 (m,1H), 4.82-3.76 (m, 4H), 3.41-2.76 (m, 2H), 1.99-1.63 (m, 3H), 1.61-1.10(m, 7H).

Example 69(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Step A: (2S,3R)-tert-butyl2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidine-1-carboxylate

A solution of intermediate B-4 (1 g),2-chloro-5-(trifluoromethyl)pyrimidine (850 mg) and DIPEA (1.6 mL) inn-BuOH (15 mL) was heated to 100° C. for 1 h. The mixture was dilutedwith H₂O and extracted with EtOAc. The combined organics were dried(MgSO₄). Purification via silica gel chromatography (0-30% EtOAc inheptane) gave the title compound (1.5 g, 89%). MS (ESI) mass calcd. forC₁₆H₂₃F₃N₄O₂, 360.24. m/z found 305.1 [M−55]⁺.

Step B:N-((2S,3R)-2-methylpiperidin-3-yl)-5-(trifluoromethyl)pyrimidin-2-amine

To the title compound of step A (1.5 g) in DCM (12 mL) was added TFA (4mL). After 1 h, the reaction was diluted with saturated Na₂CO₃ (aq) andextracted with DCM. The organic layers were dried (MgSO4) andconcentrated to give the title compound (1.03 g) that was used withoutfurther purification.

Step C:(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

To a solution of the title compound from Step B (100 mg), intermediateA-8 (118 mg) and DIPEA (0.2 mL) in DMF (5 mL) was added HBTU (218 mg).After 1 h, H₂O and EtOAc were added. The organic layer was separated anddried (MgSO4). Purification via silica gel chromatography (0-30% EtOAcin heptane) gave the title compound (86 mg, 47%). MS (ESI) mass calcd.for C₂₀H₂₁F₃N₈O, 446.2. m/z found 447.2 [M+H]⁺. MP=245.2° C. (Mixture of4 isomers, undefined ratio). ¹H NMR (300 MHz, CDCl₃) δ 8.52 (br d,J=11.5 Hz, 1.51H), 8.35 (s, 1.27H), 8.18 (br s, 0.10H), 8.09 (s, 0.46H),7.87 (d, J=5.3 Hz, 0.46H), 7.80 (d, J=7.7 Hz, 0.10H), 7.74-7.49 (m,0.87H), 7.43-7.14 (m, 1.73H), 6.87 (d, J=8.3 Hz, 0.46H), 6.61 (d, J=7.7Hz, 0.10H), 5.85 (d, J=7.0 Hz, 0.10H), 5.69 (d, J=5.5 Hz, 0.10H),5.34-5.16 (m, 0.46H), 5.01 (q, J=6.6 Hz, 0.10H), 4.73 (br d, J=14.3 Hz,0.30H), 4.29 (br d, J=6.5 Hz, 0.46H), 4.19-3.95 (m, 0.46H), 3.83 (br d,J=7.0 Hz, 0.15H), 3.65 (br s, 0.15H), 3.47 (dd, J=13.7, 4.2 Hz, 0.46H),3.26 (td, J=13.4, 3.1 Hz, 0.60H), 3.05-2.83 (m, 0.46H), 2.80 (s, 0.26H),2.78-2.64 (m, 2.16H), 2.61 (s, 0.52H), 2.52 (s, 0.26H), 1.95-1.81 (m,1.73H), 1.80-1.70 (m, 0.26H), 1.71-1.54 (m, 1.13H), 1.53-1.19 (m,3.37H), 1.08 (dd, J=14.4, 6.7 Hz, 0.10H), 1.00-0.84 (m, 0.15H), 0.74 (d,J=6.9 Hz, 0.26H).

Example 70(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-9. MS (ESI) mass calcd. for C₂₀H₂₁F₃N₈O, 446.2. m/z found447.2 [M+H]⁺. MP=181.6° C. (Mixture of 4 isomers, undefined ratio). ¹HNMR (300 MHz, CDCl₃) δ 8.66-8.15 (m, 2.14H), 7.90 (d, J=10.8 Hz, 0.45H),7.83-7.67 (m, 0.68H), 7.60 (dd, J=7.7, 2.6 Hz, 0.45H), 7.41-7.14 (m,1.53H), 6.79 (d, J=8.6 Hz, 0.26H), 6.61 (d, J=7.8 Hz, 0.16H), 5.82 (dd,J=26.8, 6.6 Hz, 0.33H), 5.17 (q, J=6.8 Hz, 0.33H), 5.05-4.86 (m, 0.26H),4.71 (t, J=15.1 Hz, 0.33H), 4.35 (br d, J=7.7 Hz, 0.45H), 4.25-4.00 (m,0.78H), 3.87 (q, J=6.9 Hz, 0.26H), 3.73 (br s, 0.26H), 3.54-3.37 (m,0.33H), 3.36-3.05 (m, 0.62H), 3.03-2.75 (m, 0.45H), 2.62 (s, 1.14H),2.59 (s, 0.76H), 2.46 (s, 0.50H), 2.18-1.83 (m, 1.62H), 1.81-1.55 (m,1.41H), 1.53-0.79 (m, 4.57H). The signal corresponding to the NH groupwas not observed.

Example 71(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-11. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/zfound 446.2 [M+H]⁺. The product is present as a mixture of conformers(ratio ca. 60:40) ¹H NMR (300 MHz, CDCl₃) δ 8.51 (d, J=10.7 Hz, 1.4H),8.40-8.28 (m, 0.9H), 8.25 (s, 1.2H), 8.00 (s, 0.6H), 7.96 (s, 0.6H),7.86-7.71 (m, 0.9H), 7.24-7.11 (m, 1.8H), 7.02-6.88 (m, 0.6H), 5.24 (q,J=7.1 Hz, 0.6H), 4.73 (d, J=13.1 Hz, 0.4H), 4.33-4.23 (m, 0.6H), 4.14(q, J=6.6 Hz, 0.4H), 4.00 (d, J=6.6 Hz, 0.4H), 3.59-3.48 (m, 0.6H),3.31-3.13 (m, 0.6H), 3.01-2.88 (m, 0.4H), 2.46 (s, 1.8H), 2.43 (s,1.2H), 2.07-1.53 (m, 4H), 1.48 (d, J=6.9 Hz, 1.2H), 1.42 (d, J=7.1 Hz,1.8H).

Example 72((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Step A: (2S,3R)-tert-butyl2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidine-1-carboxylate

To 2-chloro-5-(trifluoromethyl)pyrazine (326 μL) in DMSO (8 mL) wasadded intermediate B-4 (600 mg) and K₂CO₃ (658 mg). The reaction washeated in a microwave reactor at 120° C. for 5 min. The mixture wasdiluted with saturated NaHCO₃ (aq) and extracted with DCM. The combinedorganics were washed with brine and dried (MgSO₄). Purification viasilica gel chromatography (0-16% EtOAc in heptane) gave the titlecompound (459 mg, 46%). MS (ESI) mass calcd. for C₁₆H₂₃F₃N₄O₂, 360.24.m/z found 305.1 [M−55]⁺.

Step B:N-((2S,3R)-2-methylpiperidin-3-yl)-5-(trifluoromethyl)pyrazin-2-amine

Prepared analogous to example 69 Step B substituting the title compoundof Step A example 69 with the title compound of Step A. MS (ESI) masscalcd. for C₁₁H₁₅F₃N₄, 260.2. m/z found 261 [M+H]⁺.

Step C:((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 69 substituting intermediate A-8 withintermediate A-21. MS (ESI) mass calcd. for C₂₀H₂₁F₃N₈O, 446.2. m/zfound 447 [M+H]⁺. The product is present as a mixture of conformers(ratio ca. 70:30) ¹H NMR (300 MHz, CDCl₃) 8.46 (s, 0.3H), 8.39 (s,0.7H), 8.34 (s, 0.3H), 8.25 (s, 0.3H), 8.22 (s, 0.7H), 8.13 (s, 0.7H),8.03-7.76 (m, 3H), 7.48 (d, J=6.6 Hz, 0.7H), 6.47 (d, J=7.9 Hz, 0.3H),5.20-5.05 (m, 0.3H), 4.72-4.58 (m, 0.7H), 4.16 (br s, 0.3H), 4.08-3.87(m, 1.7H), 3.43-3.25 (m, 0.3H), 3.12-2.95 (m, 0.7H), 2.49 (s, 0.9H),2.46 (s, 2.1H), 2.08-1.63 (m, 4H), 1.50-1.47 (m, 3H).

Example 73((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 41 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-21. MS (ESI)mass calcd. for C₂₂H₂₃N₇O₂, 417.2. m/z found 418.2 [M+H]⁺. MP=112.9° C.The product is present as a mixture of conformers (ratio ca. 60:40) ¹HNMR (300 MHz, CDCl₃) δ 8.46 (s, 0.4H), 8.42 (s, 0.6H), 8.29 (s, 0.4H),8.18 (s, 0.6H), 8.09 (s, 0.8H), 7.99 (s, 1.2H), 7.72 (d, J=7.1 Hz,0.6H), 7.38 (d, J=7.8 Hz, 0.4H), 7.33-7.27 (m, 1H), 7.23-6.95 (m, 2.6H),6.64 (d, J=8.8 Hz, 0.4H), 5.34-5.16 (m, 0.4H), 4.66 (d, J=12.2 Hz,0.6H), 4.26-4.2 (m, 0.4H), 4.03 (q, J=6.8 Hz, 0.6H), 3.92 (d, J=6.7 Hz,0.6H), 3.50-3.34 (m, 0.8H), 3.12-2.94 (m, 0.6H), 2.49 (s, 1.2H), 2.43(s, 1.8H), 2.30-1.62 (m, 4H), 1.54-1.45 (m, 3H).

Example 74(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-8. MS (ESI) mass calcd. for C₂₀H₂₁F₃N₈O, 446.2. m/z found447 [M+H]⁺. MP=172.8° C. (Mixture of 4 isomers, undefined ratio). ¹H NMR(300 MHz, CDCl₃) δ 8.37 (br s, 0.21H), 8.32 (s, 0.11H), 8.21 (s, 0.40H),8.15-7.97 (m, 1.26H), 7.97-7.84 (m, 0.66H), 7.78 (d, J=7.7 Hz, 0.14H),7.74-7.53 (m, 1.46H), 7.40-7.14 (m, 1.35H), 6.54 (d, J=7.8 Hz, 0.11H),6.18 (br s, 0.30H), 5.47 (s, 0.14H), 5.28-5.09 (m, 0.39H), 5.08-4.90 (m,0.18H), 4.70 (br d, J=13.3 Hz, 0.66H), 4.22-3.94 (m, 1.59H), 3.80 (br s,0.21H), 3.64 (br s, 0.21H), 3.55-3.39 (m, 0.30H), 3.38-3.17 (m, 0.40H),3.05-2.90 (m, 0.66H), 2.89-2.79 (m, 0.18H), 2.71 (s, J=3.7 Hz, 0.92H),2.70 (s, 1.04H), 2.26-1.12 (m, 6.39H), 0.88 (t, J=6.8 Hz, 0.40H), 0.69(d, J=6.9 Hz, 0.21H). The signal corresponding to the NH group was notobserved.

Example 75((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Prepared analogous to example 73 substituting intermediate A-21 withintermediate A-8. MS (ESI) mass calcd. for C₂₂H₂₃N₇O₂, 417.2. m/z found418.2 [M+H]⁺. MP=136.3° C. The product is present as a mixture ofconformers (ratio ea. 50:50) ¹H NMR (300 MHz, CDCl₃) δ 8.25 (s, 1H),8.09 (s, 1H), 7.69-7.52 (m, 1H), 7.42-6.91 (m, 5.5H), 6.44 (d, J=8.6 Hz,0.5H), 5.39-5.22 (m, 0.5H), 4.81-4.63 (m, 0.5H), 4.27-3.91 (m, 1.5H),3.54-3.40 (m, 0.5H), 3.39-3.22 (m, 0.5H), 3.06-2.83 (m, 0.5H), 2.72 (s,1.5H), 2.67 (s, 1.5H), 2.24-1.64 (m, 4H), 1.51 (d, J=7.0 Hz, 1.5H), 1.45(d, J=7.2 Hz, 1.5H).

Example 76((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)methanone

Prepared analogous to example 73 substituting intermediate A-21 withintermediate A-9. MS (ESI) mass calcd. for C₂₂H₂₃N₇O₂, 417.2. m/z found418.2 [M+H]⁺. MP=206.3° C. The product is present as a mixture ofconformers (ratio ca. 50:50) ¹H NMR (300 MHz, CDCl₃) δ 8.60 (s, 0.5H),8.41 (s, 0.5H), 7.94 (s, 1H), 7.66-7.50 (m, 1H), 7.40-6.87 (m, 5.5H),6.55 (d, J=9.5 Hz, 0.5H), 5.28-5.17 (m, 0.5H), 4.79-4.68 (m, 0.5H),4.34-4.22 (m, 0.5H), 4.22-4.11 (m, 0.5H), 4.10-3.94 (m, 0.5H), 3.73-3.39(m, 0.5H), 3.37-3.23 (m, 0.5H), 3.04-2.89 (m, 0.5H), 2.63 (s, 1.5H),2.56 (s, 1.5H), 2.15-1.70 (m, 4H), 1.50 (d, J=7.0 Hz, 1.5H), 1.44 (d,J=7.0 Hz, 1.5H).

Example 77((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 32 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-17. MS (ESI)mass calcd. for C₂₁H₂₂ClN₅O₂, 411.1. m/z found 412.1 [M+H]⁺. MP=206.3°C. The product is present as a mixture of conformers (ratio ea, 70:30)¹H NMR (300 MHz, CDCl₃) δ 8.46 (s, 0.3H), 8.38 (s, 0.7H), 8.24 (s,0.3H), 8.13-8.02 (m, 1.7H), 7.96-7.89 (m, 2H), 7.36 (dd, J=8.9, 2.6 Hz,0.3H), 7.23 (dd, J=8.9, 2.5 Hz, 0.7H), 6.56 (d, J=7.2 Hz, 0.7H), 6.36(d, J=8.9 Hz, 0.3H), 6.22 (d, J=8.8 Hz, 0.7H), 5.72 (d, J=7.2 Hz, 0.3H),5.17-5.03 (m, 0.3H), 4.69-4.56 (m, 0.7H), 3.99-3.81 (m, 1.6H), 3.32 (q,J=13.8 Hz, 0.7H), 3.11-2.87 (m, 0.7H), 2.48 (s, 0.9H), 2.45 (s, 2.1H),2.08-1.73 (m, 3H), 1.69-1.61 (m, 1H), 1.51-1.39 (m, 3H).

Example 78((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-19. MS (ESI) mass calcd. for C₂₂H₂₂F₃N₇O, 457.2. m/zfound 458.2 [M+H]⁺. MP=116.9° C.

Example 79(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-11. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/zfound 446.2 [M+H]⁺. MP=116.2° C.

Example 80((2S,3R)-3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 42 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-17. MS (ESI)mass calcd. for C₂₁H₂₂BrN₅O₂, 455.1. m/z found 457 [M+H]⁺. MP=135.6° C.

Example 81((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 73 substituting intermediate A-21 withintermediate A-19. MS (ESI) mass calcd. for C₂₄H₂₄N₆O₂, 428.2. m/z found429.2 [M+H]⁺. MP=143.9° C.

Example 82(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-10. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O₂, 461.2. m/zfound 462.2 [M+H]⁺. MP=112.7° C.

Example 83((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-17. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O₂, 446.2. m/zfound 447.2 [M+H]⁺. MP=122.9° C.

Example 84((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-19. MS (ESI) mass calcd. for C₂₂H₂₂F₃N₇O, 457.2. m/zfound 458.2 [M+H]⁺. MP=120.6° C.

Example 85(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to example 72 substituting intermediate A-21 withintermediate A-4. MS (ESI) mass calcd. for C₂₀H₂₀F₃N₇O, 431.2. m/z found432 [M+H]⁺. MP=202.4° C.

Example 86((2S,3R)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 77 substituting 5-chloro-2-iodopyridinewith 2-chloro-5-iodopyrazine. MS (ESI) mass calcd. for C₂₀H₂₁ClN₆O₂,412.1. m/z found 413.1 [M+H]⁺.

Example 87((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 12 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-22. MS (ESI)mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/z found 446 [M+H]⁺.

Example 88((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 73 substituting intermediate A-21 withintermediate A-17. MS (ESI) mass calcd. for C₂₃H₂₃N₅O₃, 417.2. m/z found418.2 [M+H]⁺. MP=196.8° C.

Example 89(5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-14. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O₂, 461.2. m/zfound 462.2 [M+H]⁺.

Example 90(5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 72 substituting intermediate A-21 withintermediate A-24. MS (ESI) mass calcd. for C₂₀H₁₉F₄N₇O, 449.2. m/zfound 450 [M+H]⁺. MP=106.2° C.

Example 91((2S,3R)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Prepared analogous to Example 30 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-11. MS (ESI)mass calcd. for C₂₀H₂₂ClN₇O, 411.2. m/z found 412.2 [M+H]⁺. MP=166.3° C.

Example 92((2S,3R)-3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to example 42 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-21. MS (ESI)mass calcd. for C₂₀H₂₂BrN₇O, 455.2. m/z found 457 [M+H]⁺.

Example 93(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 87 substituting intermediate A-22 withintermediate A-9. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/z found446.2[M+H]⁺. MP=159.8° C.

Example 94((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-21. MS (ESI) mass calcd. for C₂₀H₂₁F₃N₈O, 446.2. m/zfound 447 [M+H]⁺. MP=101.2° C.

Example 95(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 69 substituting intermediate A-8 withintermediate A-10. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O₂, 461.2. m/zfound 462.2 [M+H]⁺. The product is present as a mixture of conformers(ratio ca. 60:40) ¹H NMR (300 MHz, CDCl₃) δ 8.51 (d, J=10.0 Hz, 1.4H),8.37 (d, J=10.3 Hz, 0.6H), 8.25 (s, 1.4H), 8.00 (s, 0.4H), 7.78 (d,J=4.4 Hz, 0.4H), 7.67 (d, J=2.3 Hz, 0.6H), 7.46 (d, J=2.3 Hz, 0.4H),7.24 (d, J=9.3 Hz, 0.6H), 7.17 (d, J=8.5 Hz, 0.6H), 7.03-6.85 (m, 1.6H),5.24 (q, J=6.7 Hz, 0.6H), 4.72 (d, J=13.2 Hz, 0.4H), 4.27 (d, J=6.7 Hz,0.6H), 4.22-4.08 (m, 0.4H), 4.01 (d, J=6.4 Hz, 0.4H), 3.91 (s, 1.8H),3.87 (s, 1.2H), 3.55 (dd, J=13.6, 3.7 Hz, 0.6H), 3.23 (td, J=13.4, 3.0Hz, 0.6H), 2.99-2.90 (m, 0.4H), 2.09-1.52 (m, 4H), 1.48 (d, J=6.9 Hz,1.2H), 1.42 (d, J=7.1 Hz, 1.8H).

Example 96(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 72 substituting intermediate A-21 withintermediate A-9. MP=149.6° C.

Example 97(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 87 substituting intermediate A-22 withintermediate A-10. MS (ESI) mass calcd. for C₂₂H₂₃F₃N₆O₂, 460.2. m/zfound [M+H]⁺.

Example 98(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Prepared analogous to Example 87 substituting intermediate A-22 withintermediate A-8. MS (ESI) mass calcd. for C₂₁H₂₂F₃N₇O, 445.2. m/z found446 [M+H]⁺.

Example 99((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Prepared analogous to Example 32 substituting intermediate B-3 withintermediate B-4 and intermediate A-4 with intermediate A-11. MS (ESI)mass calcd. for C₂₁H₂₃ClN₆O, 410.2. m/z found 411.2 [M+H]⁺.

Example 100(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 32 substituting intermediate B-3 withintermediate B-4. MS (ESI) mass calcd. for C₂₀H₂₁ClN₆O, 396.1. m/z found397 [M+H]⁺. MP=140.1° C.

Example 101((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Step A: (2S,3R)-tert-butyl2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidine-1-carboxylate

To (2S,3R)-tert-butyl 3-hydroxy-2-methylpiperidine-1-carboxylate (670mg, prepared according to J. Org. Chem. 2008, 73, 2898) in THF (15 mL)at 0° C. was added NaH (60 wt %, 188 mg). After 15 min,2-chloro-5-(trifluoromethyl)pyrimidine (570 mg) was added and thereaction allowed to proceed at rt overnight. The reaction was dilutedwith H₂O and extracted with DCM. The organic layers were dried (MgSO₄).Purification via silica gel chromatography (0-40% EtOAc in heptane) gavethe title compound (813 mg, 72%).

Step B:2-(((2S,3R)-2-methylpiperidin-3-yl)oxy)-5-(trifluoromethyl)pyrimidine

To the title compound of step A (813 mg) in DCM (6 mL) was added TFA (2mL). After 1 h, the reaction was diluted with saturated Na₂CO₃ (aq) andextracted with DCM. The organic layers were dried (MgSO₄) andconcentrated to give the title compound (452 mg) that was used withoutfurther purification.

Step C:((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

To a solution of the title compound from Step B (100 mg), intermediateA-21 (118 mg) and DIPEA (0.2 mL) in DMF (5 mL) was added HBTU (218 mg).After 1 h, H₂O and EtOAc were added. The organic layer was separated anddried (MgSO₄). Purification via prep HPLC gave the title compound (62mg, 36%). MS (ESI) mass calcd. for C₂₀H₂₀F₃N₂O₂, 447.2. m/z found 448.2[M+H]⁺. MP=214.9° C.

Example 102(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to Example 101 substituting intermediate A-21 withintermediate A-11. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O₂, 446.2. m/zfound 447.2 [M+H]⁺. MP=163.7° C.

Example 103(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to Example 101 substituting intermediate A-21 withintermediate A-4. MS (ESI) mass calcd. for C₂₀H₁₉F₃N₆O₂, 432.2. m/zfound 433 [M+H]⁺.

Example 104((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 101 substituting intermediate A-21 withintermediate A-22. MS (ESI) mass calcd. for C₂₀H₂₀F₃N₇O₂, 447.2. m/zfound 448 [M+H]⁺. MP=161.5° C.

Example 105((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 101 substituting2-chloro-5-(trifluoromethyl)pyrimidine with2-chloro-5-(trifluoromethyl)pyrazine. MS (ESI) mass calcd. forC₂₀H₂₀F₃N₇O₂, 447.2. m/z found 448 [M+H]⁺. MP=149.7° C.

Example 106(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to Example 105 substituting intermediate A-21 withintermediate A-4. MS (ESI) mass calcd. for C₂₀H₁₉F₃N₆O₂, 432.2. m/zfound 433.2 [M+H]⁺. MP=111.2° C.

Example 107((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 105 substituting intermediate A-21 withintermediate A-22. MS (ESI) mass calcd. for C₂₀H₂₀F₃N₇O₂, 447.2. m/zfound 448 [M+H]⁺. MP=161.5° C.

Example 108((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Step A: (2S,3R)-tert-butyl2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidine-1-carboxylate

To (2S,3R)-tert-butyl 3-hydroxy-2-methylpiperidine-1-carboxylate (1.5 g,6.97 mmol, prepared according to J. Org. Chem. 2008, 73, 2898) in THF(20 mL) at 0° C. was added NaH (60 wt %, 418 mg, 10.5 mmol). After 15min, 2-chloro-5-(trifluoromethyl)pyridine (1.33 g, 7.32 mmol) was addedand the reaction allowed to proceed at rt overnight. The reaction wasdiluted with H₂O and extracted with EtOAc. The combined organic layerswere dried (MgSO₄), filtered and concentrated. Purification via silicagel chromatography (0-25% EtOAc in heptane) gave the title compound(2.37 g, 94%, 97% purity). MS (ESI) mass calcd. for C₁₂H₂₃F₃N₂O₃, 360.2.m/z found 361.0 [M+H]⁺.

Step B:2-(((2S,3R)-2-methylpiperidin-3-yl)oxy)-5-(trifluoromethyl)pyridine

To the title compound of step A (3.45 g, 9.57 mmol) in DCM (37 mL) wasadded TFA (12 mL), and the reaction mixture was stirred at rt for 1 h.Then, the crude reaction was diluted with saturated Na₂CO₃ (aq) andextracted with DCM. The organic layers were dried (MgSO₄) andconcentrated to give the title compound (2.34 g) that was used withoutfurther purification. MS (ESI) mass calcd. for C₁₂H₁₅F₃N₂O, 260.1. m/zfound 261 [M+H]⁺.

Step C:((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

To a solution of the title compound from Step B (2.34 g), intermediateA-22 (4.58 g, 10.8 mmol) and DIPEA (4.6 mL, 27 mmol) in DMF (27 mL) wasadded HBTU (5.10 g, 13.5 mmol). After 1 h, a solution of saturatedNaHCO₃ (aq) and EtOAc were added. The reaction mixture was extractedwith EtOAc (3×). The combined organic layer was dried (MgSO₄), filteredand concentrated. Purification via prep silica gel chromatography (0-50%EtOAc in heptane) gave the title compound as crystalline material (3.43g, 70%, 98% purity). MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O₂, 446.2. m/zfound 447 [M+H]⁺. ¹H NMR (500 MHz, Chloroform-d) δ 8.45-8.36 (m, 0.45H),8.16 (d, J=8.4 Hz, 0.45H), 8.15-8.13 (m, 0.55H), 8.07 (d, J=8.4 Hz,0.55H), 7.81 (s, 1.1H), 7.80 (s, 0.9H), 7.77 (dd, J=8.7, 2.6 Hz, 0.45H),7.68 (dd, J=8.7, 2.6 Hz, 0.55H), 7.30 (d, J=8.4 Hz, 0.45H), 7.11 (d,J=8.5 Hz, 0.55H), 6.96-6.88 (m, 0.45H), 6.68 (d, J=8.7 Hz, 0.55H),5.31-5.25 (m, 0.45H), 5.15-5.08 (m, 0.45H), 4.89-4.82 (m, 0.55H),4.79-4.72 (m, 0.55H), 4.08-3.99 (m, 0.55H), 3.38-3.29 (m, 0.45H),3.13-3.03 (m, 0.45H), 2.94 (td, J=13.4, 3.2 Hz, 0.55H), 2.65 (s, 1.3H),2.25 (s, 1.7H), 2.19-1.86 (m, 3H), 1.67-1.61 (m, 0.55H), 1.47-1.32 (m,1.8H), 1.06 (d, J=7.0 Hz, 1.65H).

Example 109(±)-trans-(2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 108 using (±)-trans-tert-butyl3-hydroxy-2-methylpiperidine-1-carboxylate. MS (ESI) mass calcd. forC₂₁H₂₁F₃N₆O₂, 446.2. m/z found 447.2 [M+H]⁺. MP=161.3° C.

Example 110(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to Example 108 substituting intermediate A-22 withintermediate A-4. MS (ESI) mass calcd. for C₂₁H₂₀F₃N₅O₂, 431.2. m/zfound 432.2 [M+H]⁺. MP=159.7° C.

Example 111((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 108 substituting intermediate A-22 withintermediate A-21. MP=281.7° C.

Example 112(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to example 108 substituting intermediate A-22 withintermediate A-11. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O₂, 446.2. m/zfound 447 [M+H]⁺. MP=167.1° C.

Example 113(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

Prepared analogous to Example 105 substituting intermediate A-21 withintermediate A-11. MS (ESI) mass calcd. for C₂₁H₂₁F₃N₆O₂, 446.2. m/zfound 447 [M+H]⁺. MP=167.1° C.

The following compounds are also within the scope of the invention. Theycan be prepared using methods known in the art, in combination with theexperimental details and schemes provided herein.

Example 114(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 115(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 116(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-28. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.82 (d, J=5.0 Hz, 2H), 8.39 (s, 0.6H), 8.21 (s, 0.4H), 7.90-7.70 (m,1H), 7.55-7.40 (m, 0.6H), 7.25-7.17 (m, 2H), 7.07-6.98 (m, 0.4H),6.95-6.74 (m, 2H), 5.22 (s, 0.6H), 5.05-4.87 (m, 1H), 4.63-4.47 (m,0.4H), 4.25-4.08 (m, 0.4H), 3.62-3.44 (m, 0.6H), 3.10 (td, J=13.2, 3.3Hz, 0.6H), 2.75 (t, J=13.1 Hz, 0.4H), 2.14-1.78 (m, 3H), 1.62-1.54 (m,0.6H), 1.49-1.36 (m, 0.4H), 1.25-1.12 (m, 1.6H), 1.06-0.94 (m, 1.4H).

Example 117(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Example 118(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

Example 119((2S,3R)-3-(benzo[d]oxazol-2-yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(pyrimidin-2-yl)phenyl)methanone

Example 120((2S,3R)-3-(benzo[d]oxazol-2-yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Example 121(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 122(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 123(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Example 124(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-3. MS (ESI): mass calcd. forC₂H₁₉F₄N₅O₂, 449.1. m/z found, 449.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.42-8.36 (m, 0.55H), 8.23-8.18 (m, 0.45H), 7.96-7.72 (m, 3H), 7.57-7.46(m, 0.55H), 7.40-7.27 (m, 1H), 7.18-7.08 (m, 0.45H), 6.96-6.76 (m, 2H),5.26-5.19 (m, 0.55H), 5.05-4.88 (m, 1H), 4.61-4.52 (m, 0.45H), 4.15-4.01(m, 0.55H), 3.49-3.36 (m, 0.45H), 3.03-2.90 (m, 0.55H), 2.82-2.68 (m,0.45H), 2.08-1.76 (m, 3H), 1.46-1.21 (m, 1H), 1.15 (d, J=7.1 Hz, 1.45H),0.98 (d, J=7.0 Hz, 1.55H).

Example 125(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substituting2-chloro-5-(trifluoromethyl)pyridine with2-chloro-5-(trifluoromethyl)pyrazine, and intermediate A-1 withintermediate A-3. MS (ESI): mass calcd. for C₂₀H₁₈F₄N₆O₂, 450.1. m/zfound, 450.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.44-8.38 (m, 0.6H), 8.30(s, 0.4H), 8.26 (s, 0.6H), 8.21 (s, 0.4H), 7.87 (s, 1H), 7.85 (s, 1H),7.58-7.47 (m, 0.6H), 7.38-7.26 (m, 1.4H), 7.17-7.07 (m, 0.4H), 6.97-6.85(m, 0.6H), 5.24-5.14 (m, 0.6H), 5.07-4.97 (m, 0.6H), 4.95-4.82 (m,0.4H), 4.65-4.51 (m, 0.4H), 4.04-3.87 (m, 0.6H), 3.47-3.37 (m, 0.6H),3.05-2.89 (m, 0.4H), 2.82-2.64 (m, 0.4H), 2.15-1.78 (m, 3H), 1.65-1.56(m, 0.4H), 1.51-1.40 (m, 0.6H), 1.14 (d, J=7.1 Hz, 2H), 0.90 (d, J=7.0Hz, 1H).

Example 126((2S,3R)-3-(benzo[d]oxazol-2-yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Example 127((2S,3R)-3-(benzo[d]oxazol-2-yloxy)-2-methylpiperidin-1-yl)(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)methanone

Example 128(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 129(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 130(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Example 131(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 132(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

Example 133(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

Example 134(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 135(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

Example 136(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 137(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 138(5-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-7. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 461.2 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.76 (d, J=4.8 Hz, 1H), 8.72 (d, J=4.9 Hz, 1H), 8.50-8.35 (m, 1H), 8.23(dd, J=8.8, 5.6 Hz, 0.5H), 8.17 (s, 0.5H), 7.84 (dd, J=8.7, 2.5 Hz,0.5H), 7.79 (dd, J=8.8, 2.5 Hz, 0.5H), 7.24-7.15 (m, 1.5H), 7.10 (dd,J=8.5, 2.7 Hz, 0.5H), 6.96 (td, J=8.4, 2.7 Hz, 0.5H), 6.90-6.84 (m, 1H),6.81 (dd, J=8.5, 2.7 Hz, 0.5H), 5.34-5.30 (m, 0.5H), 5.15-5.05 (m,0.5H), 4.93-4.85 (m, 0.5H), 4.79-4.71 (m, 0.5H), 4.11-4.00 (m, 0.5H),3.44-3.33 (m, 0.5H), 3.18-3.04 (m, 0.6H), 2.95-2.89 (m, 0.4H), 2.16-1.84(m, 3H), 1.70-1.60 (m, 0.5H), 1.48-1.33 (m, 2H), 0.90 (d, J=7.0 Hz,1.5H).

Example 139(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-37. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 461.2 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.78 (d, J=4.8 Hz, 0.9H), 8.75 (d, J=4.8 Hz, 1.1H), 8.42 (s, 0.6H), 8.19(s, 0.4H), 8.11 (dd, J=10.2, 2.7 Hz, 0.4H), 7.95 (d, J=10.1 Hz, 0.6H),7.84 (d, J=8.7 Hz, 0.4H), 7.78 (dd, J=8.8, 2.6 Hz, 0.6H), 7.38 (dd,J=8.4, 5.5 Hz, 0.6H), 7.25-7.17 (m, 1.6H), 7.09-7.01 (m, 0.4H), 6.86 (d,J=8.7 Hz, 1H), 6.64-6.55 (m, 0.4H), 5.35-5.28 (m, 0.6H), 5.17-5.07 (m,0.6H), 4.89 (s, 0.4H), 4.82-4.67 (m, 0.4H), 4.10-4.01 (m, 0.4H),3.44-3.31 (m, 0.6H), 3.15-3.00 (m, 0.6H), 2.94-2.89 (m, 0.4H), 2.13-1.81(m, 3H), 1.70-1.61 (m, 0.4H), 1.47-1.32 (m, 2.2H), 0.91 (d, J=7.0 Hz,1.4H).

Example 140(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 141(2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-25. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 461.2 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.72-8.56 (m, 2H), 8.45-8.40 (m, 0.4H), 8.34-8.28 (m, 0.4H), 8.22-8.10(m, 1.2H), 7.88-7.81 (m, 0.4H), 7.80-7.74 (m, 0.4H), 7.55-7.45 (m,1.2H), 7.45-7.36 (m, 0.4H), 7.33-7.26 (m, 0.6H), 7.11-7.00 (m, 0.4H),6.92-6.82 (m, 1.6H), 5.38-5.28 (m, 0.6H), 5.17-5.07 (m, 0.4H), 4.89 (s,0.4H), 4.81-4.71 (m, 0.4H), 4.15-4.03 (m, 0.6H), 3.50-3.37 (m, 0.6H),3.16-3.03 (m, 0.6H), 2.98-2.84 (m, 0.4H), 2.18-1.83 (m, 3H), 1.78-1.60(m, 0.6H), 1.47-1.31 (m, 2.2H), 0.94 (d, J=7.0 Hz, 1.2H).

Example 142(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 143((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 101 steps A and B,substituting 2-chloro-5-(trifluoromethyl)pyrimidine with2-chloro-5-(trifluoromethyl)pyridine. Step C:((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone.To 2-(((2S,3R)-2-methylpiperidin-3-yl)oxy)-5-(trifluoromethyl)pyridine(48 mg, 0.181 mmol) and intermediate A-29 (43 mg, 0.217 mmol) was addedDCM (1 mL) and DIPEA (0.04 mL, 0.235 mmol). T₃P (0.325 mL, 0.542 mmol,50% solution in DMF) was then added dropwise and the mixture heated to45° C. Upon completion the reaction was quenched with saturated NaHCO₃solution and the aqueous layer extracted with EtOAc (3×). The combinedorganics were washed saturated NaHCO₃ solution, brine, dried with MgSO₄,filtered, and concentrated. Purification via silica gel chromatography(0-100% EtOAc in hexanes) gave the title compound (67.2 mg, 84%). MS(ESI): mass calcd. for C₂₃H₂₁F₃N₄O₂, 442.2. m/z found 443.2 [M+H]⁺. ¹HNMR (400 MHz, CDCl₃) δ 8.78 (d, J=4.8 Hz, 1.1H), 8.74 (d, J=4.9 Hz,0.9H), 8.42 (s, 0.5H), 8.39-8.33 (m, 0.5H), 8.20 (d, J=8.0 Hz, 0.5H),8.13 (s, 0.5H), 7.86-7.81 (m, 0.5H), 7.78 (dd, J=8.8, 2.6 Hz, 0.5H),7.54-7.47 (m, 1H), 7.44-7.38 (m, 0.5H), 7.31-7.27 (m, 0.5H), 7.18 (t,J=4.8 Hz, 1H), 7.05 (d, J=7.5 Hz, 0.5H), 6.91-6.83 (m, 1.5H), 5.35-5.28(m, 0.5H), 5.18-5.08 (m, 0.5H), 4.94-4.83 (m, 0.5H), 4.82-4.72 (m,0.5H), 4.13-4.01 (m, 0.5H), 3.49-3.34 (m, 0.5H), 3.17-3.02 (m, 0.5H),2.94-2.8 (m, 0.5H), 2.17-1.81 (m, 3H), 1.68-1.61 (m, 0.5H), 1.45-1.32(m, 2H), 0.88 (d, J=7.0 Hz, 1.5H).

Example 144(2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Example 145(4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Example 146(3-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

Example 147(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 101 steps A and B,substituting 2-chloro-5-(trifluoromethyl)pyrimidine with2-chloro-5-(trifluoromethyl)pyridine. Step C:(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone.To 2-(((2S,3R)-2-methylpiperidin-3-yl)oxy)-5-(trifluoromethyl)pyridine(50 mg, 0.169 mmol) in DCM (5 mL) was intermediate A-1 (38 mg, 0.185mmol), HOBt (38 mg, 0.279 mmol), EDCI (53 mg, 0.279 mmol) and DIPEA (72μL, 0.418 mmol). After stirring at room temperature for 2 h, saturatedNaHCO₃ (aq.) was added and the mixture was extracted with DCM (3×). Thecombined organics were dried (Na₂SO₄), filtered and concentrated.Purification via silica gel chromatography (0-100% EtOAc in hexanes)gave the title compound (66.6 mg, 88%). MS (ESI): mass calcd. forC₂₁H₁₉F₄N₅O₂, 449.1. m/z found, 449.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.42-8.41 (m, 0.5H), 8.23-8.16 (m, 0.5H), 7.89-7.75 (m, 3.6H), 7.65 (dd,J=9.5, 2.5 Hz, 0.5H), 7.44 (dd, J=8.5, 5.8 Hz, 0.4H), 7.15 (td, J=8.2,2.5 Hz, 0.5H), 7.05 (dd, J=8.5, 5.8 Hz, 0.5H), 6.84 (d, J=8.7 Hz, 1H),6.56-6.48 (m, 0.5H), 5.32-5.24 (m, 0.5H), 5.16-5.09 (m, 0.5H), 4.91-4.84(m, 0.5H), 4.77-4.68 (m, 0.5H), 4.05-3.94 (m, 0.5H), 3.37-3.28 (m,0.5H), 2.99-2.81 (m, 1H), 2.18-1.80 (m, 3H), 1.68-1.61 (m, 0.6H),1.42-1.34 (m, 1.9H), 0.83 (d, J=7.0 Hz, 1.5H).

Example 148(2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 125 substitutingintermediate A-3 with intermediate A-31. MS (ESI): mass calcd. forC₁₉H₁₈F₃N₇O₂, 433.1. m/z found, 433.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.66 (dd, J=4.8, 1.8 Hz, 0.5H), 8.48-8.44 (m, 0.5H), 8.42 (dd, J=4.8,1.8 Hz, 0.5H), 8.31-8.26 (m, 1H), 8.20-8.14 (m, 0.5H), 7.95-7.83 (m,2.5H), 7.50 (dd, J=7.6, 1.8 Hz, 0.5H), 7.45 (dd, J=7.7, 4.8 Hz, 0.5H),6.82 (dd, J=7.6, 4.7 Hz, 0.5H), 5.31-5.24 (m, 0.5H), 5.20-5.09 (m,0.5H), 4.86-4.80 (m, 0.5H), 4.80-4.69 (m, 0.5H), 3.90-3.79 (m, 0.5H),3.33-3.24 (m, 0.5H), 3.03-2.86 (m, 1H), 2.11-1.65 (m, 3.5H), 1.48-1.34(m, 2.5H), 0.82 (d, J=7.0 Hz, 1H).

Example 149(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-30. MS (ESI): mass calcd. forC₂₁H₂₁F₃N₆O₂, 446.2. m/z found, 446.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.49-8.44 (m, 0.4H), 8.43-8.41 (m, 0.4H), 8.27-8.22 (m, 0.6H), 8.21-8.19(m, 0.6H), 7.87 (s, 1.2H), 7.86-7.81 (m, 1.4H), 7.78 (dd, J=8.8, 2.6 Hz,0.4H), 7.67-7.63 (m, 0.4H), 7.31 (dd, J=2.3, 0.8 Hz, 0.6H), 6.87-6.80(m, 1H), 5.30-5.26 (m, 0.4H), 5.17-5.07 (m, 0.4H), 4.88-4.82 (m, 0.6H),4.78-4.69 (m, 0.6H), 4.05-3.95 (m, 0.6H), 3.30-3.21 (m, 0.4H), 2.97-2.85(m, 1H), 2.46 (s, 1.2H), 2.11-1.83 (m, 4.8H), 1.73-1.62 (m, 0.6H), 1.38(d, J=7.3 Hz, 1.6H), 0.83 (d, J=7.0 Hz, 1.8H).

Example 150(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-8. MS (ESI): mass calcd. forC₂₁H₂₁F₃N₆O₂, 446.2. m/z found, 446.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.43-8.39 (m, 0.4H), 8.15-8.10 (m, 0.6H), 7.87 (s, 1H), 7.86-7.81 (m,1.4H), 7.80-7.73 (m, 1H), 7.32-7.27 (m, 1H), 6.86-6.79 (m, 1H), 6.58 (d,J=7.8 Hz, 0.6H), 5.30-5.25 (m, 0.4H), 5.16-5.06 (m, 0.4H), 4.85-4.77 (m,0.6H), 4.77-4.67 (m, 0.6H), 4.03-3.89 (m, 0.6H), 3.33-3.25 (m, 0.4H),2.97-2.83 (m, 1H), 2.70 (s, 1.2H), 2.52 (s, 1.8H), 2.13-1.70 (m, 3.6H),1.42-1.34 (m, 1.6H), 0.79 (d, J=7.0 Hz, 1.8H).

Example 151(3-methyl-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-27. MS (ESI): mass calcd. forC₂₄H₂₃F₃N₄O₂, 456.2. m/z found, 457.2 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.91-8.71 (m, 2H), 8.37 (s, 0.6H), 8.24 (s, 0.4H), 7.89-7.80 (m, 0.4H),7.75 (d, J=8.8 Hz, 0.6H), 7.41-7.09 (m, 3.4H), 6.94 (dd, J=38.0, 8.2 Hz,0.6H), 6.88-6.72 (m, 1H), 5.19 (s, 0.6H), 5.00 (s, 0.4H), 4.98-4.87 (m,0.6H), 4.55-4.43 (m, 0.4H), 4.35-4.23 (m, 0.4H), 3.67-3.53 (m, 0.6H),3.13-3.01 (m, 0.6H), 2.95 (s, 1.8H), 2.88 (s, 1.2H), 2.79-2.66 (m,0.4H), 2.27 (s, 1H), 2.04-1.79 (m, 2.4H), 1.51-1.35 (m, 0.6H), 1.22-1.00(m, 3H).

Example 152(3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-25. MS (ESI): mass calcd. forC₂₂H₂₂F₃N₅O₂, 445.2. m/z found, 446.2 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.38 (s, 0.6H), 8.24 (s, 0.4H), 7.95-7.78 (m, 3.4H), 7.75 (dd, J=8.8,2.4 Hz, 0.6H), 7.58-7.30 (m, 1H), 7.20-7.16 (m, 0.5H), 6.97 (d, J=7.6Hz, 0.5H), 6.88 (d, J=8.7 Hz, 0.4H), 6.84-6.78 (m, 0.6H), 5.21-5.16 (m,0.6H), 5.01-4.98 (m, 0.4H), 4.97-4.89 (m, 0.6H), 4.56-4.46 (m, 0.4H),4.17-4.10 (m, 0.4H), 3.49-3.41 (m, 0.6H), 3.03-2.95 (m, 0.6H), 2.79-2.65(m, 0.4H), 2.20 (s, 1.6H), 2.12 (s, 1.4H), 2.03-1.76 (m, 3H), 1.44-1.23(m, 1H), 1.14 (d, J=7.0 Hz, 1.2H), 1.04 (d, J=7.1 Hz, 1.8H).

Example 153(2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 147 substitutingintermediate A-1 with intermediate A-31. MS (ESI): mass calcd. forC₂₀H₁₉F₃N₆O₂, 432.2. m/z found, 433.2 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.66 (dd, J=4.7, 1.8 Hz, 0.4H), 8.47-8.38 (m, 1.1H), 8.19-8.11 (m,0.5H), 7.89 (s, 1H), 7.89-7.86 (m, 1.6H), 7.84 (dd, J=8.8, 2.7 Hz,0.4H), 7.78 (dd, J=8.8, 2.5 Hz, 0.4H), 7.49-7.42 (m, 1.2H), 6.85-6.82(m, 0.9H), 6.77 (dd, J=7.6, 4.8 Hz, 0.5H), 5.36-5.28 (m, 0.4H),5.16-5.06 (m, 0.4H), 4.87-4.81 (m, 0.6H), 4.77-4.70 (m, 0.6H), 4.01-3.90(m, 0.6H), 3.30-3.24 (m, 0.4H), 3.05-2.87 (m, 1H), 2.14-1.79 (m, 3H),1.70-1.62 (m, 0.6H), 1.41-1.33 (m, 1.9H), 0.86 (d, J=7.0 Hz, 1.5H).

Example 154((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 143 substituting2-chloro-5-(trifluoromethyl)pyridine with2-chloro-3-fluoro-5-(trifluoromethyl)pyridine. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.78 (d, J=4.8 Hz, 0.8H), 8.74 (d, J=4.9 Hz, 1.2H), 8.39-8.32 (m, 0.6H),8.27-8.17 (m, 1H), 7.95 (s, 0.4H), 7.62-7.45 (m, 2H), 7.45-7.38 (m,0.5H), 7.33-7.25 (m, 1H), 7.22-7.15 (m, 1H), 7.05-6.98 (m, 0.5H),5.41-5.36 (m, 0.6H), 5.22-5.11 (m, 0.6H), 4.97-4.93 (m, 0.4H), 4.85-4.75(m, 0.4H), 4.09-3.99 (m, 0.4H), 3.48-3.36 (m, 0.6H), 3.17-3.04 (m,0.6H), 2.95-2.90 (m, 0.4H), 2.14-1.91 (m, 3H), 1.72-1.59 (m, 0.5H),1.46-1.35 (m, 2.3H), 0.93 (d, J=7.0 Hz, 1.2H).

Example 155(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

The title compound was prepared analogous to Example 154 substitutingintermediate A-29 with intermediate A-28. MS (ESI): mass calcd. forC₂₃H₁₉F₅N₄O₂, 478.1. m/z found, 478.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.32 min at 254 nm.

Example 156((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(5-fluoropyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 154 substitutingintermediate A-29 with intermediate A-25. MS (ESI): mass calcd. forC₂₃H₁₉F₅N₄O₂, 478.1. m/z found, 478.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.85 min at 254 nm.

Example 157((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

The title compound was prepared analogous to Example 147 substituting2-chloro-5-(trifluoromethyl)pyridine with2-chloro-3-fluoro-5-(trifluoromethyl)pyridine, and substitutingintermediate A-1 with intermediate A-30. MS (ESI): mass calcd. forC₂₁H₂₀F₄N₆O₂, 464.2. m/z found, 465.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.51-8.40 (m, 0.5H), 8.34-8.26 (m, 0.5H), 8.25-8.18 (m, 0.5H), 8.11-8.03(m, 0.5H), 7.87 (s, 1.2H), 7.84 (s, 0.8H), 7.69-7.65 (m, 1H), 7.63-7.53(m, 1H), 5.43-5.36 (m, 0.4H), 5.21-5.11 (m, 0.4H), 5.06-4.96 (m, 0.6H),4.84-4.68 (m, 0.6H), 4.01-3.89 (m, 0.6H), 3.34-3.24 (m, 0.4H), 3.05-2.88(m, 1H), 2.46 (s, 1.2H), 2.19 (s, 1.8H), 2.14-1.92 (m, 3H), 1.69-1.61(m, 0.5H), 1.50-1.33 (m, 1.7H), 0.91 (d, J=7.0 Hz, 1.8H).

Example 158((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 157 substitutingintermediate A-30 with intermediate A-15. MS (ESI): mass calcd. forC₂₂H₂₁F₄N₅O₂, 463.2. m/z found, 463.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.18-8.15 (m, 0.6H), 8.09-8.05 (m, 0.4H), 7.82 (s, 0.8H), 7.79 (s,1.2H), 7.65-7.59 (m, 0.4H), 7.51 (dd, J=9.4, 2.1 Hz, 0.6H), 7.45-7.31(m, 2H), 7.27-7.18 (m, 0.6H), 7.02-6.95 (m, 0.4H), 5.28-5.23 (m, 0.6H),5.09-5.05 (m, 0.4H), 5.04-4.91 (m, 0.6H), 4.57-4.49 (m, 0.4H), 4.26-4.07(m, 0.4H), 3.52-3.40 (m, 0.6H), 3.11-2.94 (m, 0.6H), 2.79-2.68 (m,0.4H), 2.20 (s, 1.8H), 2.13 (s, 1.2H), 2.04-1.85 (m, 3H), 1.48-1.25 (m,1H), 1.16 (d, J=7.0 Hz, 1.2H), 1.07 (d, J=7.2 Hz, 1.8H).

Example 159(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

The title compound was prepared analogous to Example 157 substitutingintermediate A-30 with intermediate A-3. MS (ESI): mass calcd. forC₂₁H₁₈F₅N₅O₂, 467.1. m/z found, 467.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.18 (s, 0.6H), 8.05 (s, 0.4H), 7.87 (s, 0.8H), 7.84 (s, 1.2H),7.64-7.43 (m, 2H), 7.37-7.28 (m, 1H), 7.25-7.04 (m, 1H), 5.35-5.27 (m,0.6H), 5.09-4.97 (m, 1H), 4.64-4.56 (m, 0.4H), 4.12-3.98 (m, 0.4H),3.47-3.38 (m, 0.6H), 3.07-2.96 (m, 0.6H), 2.86-2.71 (m, 0.4H), 2.10-1.90(m, 3H), 1.16 (d, J=7.1 Hz, 1.8H), 1.02 (d, J=7.0 Hz, 1.2H). *1H buriedunder water peak.

Example 160((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 157 substitutingintermediate A-30 with intermediate A-22. MS (ESI): mass calcd. forC₂₁H₂₀F₄N₆O₂, 464.2. m/z found, 465.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.24-8.19 (m, 0.5H), 8.16 (d, J=8.4 Hz, 0.5H), 8.05 (d, J=8.4 Hz, 0.5H),7.91-7.87 (m, 0.5H), 7.81 (s, 1H), 7.77 (s, 1H), 7.53 (dd, J=9.7, 2.2Hz, 0.5H), 7.39 (dd, J=9.6, 2.1 Hz, 0.5H), 7.28 (d, J=8.5 Hz, 0.5H),7.13 (d, J=8.5 Hz, 0.5H), 5.36-5.31 (m, 0.5H), 5.24-5.15 (m, 0.5H),4.92-4.84 (m, 0.5H), 4.80-4.71 (m, 0.5H), 4.23-4.13 (m, 0.5H), 3.39-3.30(m, 0.5H), 3.20-3.09 (m, 0.5H), 2.98 (td, J=13.4, 3.2 Hz, 0.5H), 2.63(s, 1.6H), 2.30 (s, 1.4H), 2.12-1.98 (m, 3H), 1.76-1.66 (m, 0.5H),1.46-1.37 (m, 2H), 1.17 (d, J=7.0 Hz, 1.5H).

Example 161((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 147 substituting2-chloro-5-(trifluoromethyl)pyridine with 2,5-dichloropyrimidine, andsubstituting intermediate A-1 with intermediate A-3. MS (ESI): masscalcd. for C₁₉H₁₈ClFN₆O₂, 416.1. m/z found, 417.0 [M+H]⁺. ¹HNMR containsa mixture of more than two rotamers. Analytical HPLC was obtained on aAgilent 1100 Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm),mobile phase of 5-99% ACN in 0.05% TFA over 7 min and then hold at 99%ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50° C.).R_(t)=6.44 min at 254 nm.

Example 162((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

The title compound was prepared analogous to Example 161 substitutingintermediate A-3 with intermediate A-30. MS (ESI): mass calcd. forC₁₉H₂₀ClN₇O₂, 413.1. m/z found, 414.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃,multiple rotamers present, two major rotamers reported) δ 8.45 (s, 1H),8.42 (d, J=2.1 Hz, 0.5H), 8.35 (s, 1H), 8.26 (d, J=2.1 Hz, 0.5H), 7.86(s, 1H), 7.82 (s, 1H), 7.70 (dd, J=2.2, 0.9 Hz, 0.5H), 7.61 (dd, J=2.2,0.9 Hz, 0.5H), 5.20-5.14 (m, 0.5H), 5.12-5.08 (m, 0.5H), 4.79-4.66 (m,1H), 4.02-3.88 (m, 0.5H), 3.36-3.21 (m, 0.5H), 2.91 (td, J=13.3, 3.1 Hz,1H), 2.42 (s, 1.5H), 2.16 (s, 1.5H), 2.03-1.83 (m, 3H), 1.44-1.35 (m,2.5H), 0.89 (d, J=7.0 Hz, 1.5H).

Example 163((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 161 substitutingintermediate A-3 with intermediate A-15. MS (ESI): mass calcd. forC₂₀H₂₁ClN₆O₂, 412.1. m/z found, 412.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃,multiple rotamers present, two major rotamers reported) δ 8.42-8.36 (m,2H), 7.84-7.74 (m, 2H), 7.45-7.33 (m, 2H), 7.22-7.13 (m, 0.7H), 6.90 (t,J=7.6 Hz, 0.3H), 5.09-4.92 (m, 1.4H), 4.85-4.77 (m, 0.3H), 4.55-4.44 (m,0.3H), 4.18-4.10 (m, 0.3H), 3.53-3.37 (m, 0.7H), 3.09-2.93 (m, 0.7H),2.78-2.65 (m, 0.3H), 2.19 (s, 2.1H), 2.11 (s, 0.9H), 2.10-1.77 (m, 3H),1.49-1.28 (m, 1H), 1.16 (d, J=7.0 Hz, 0.9H), 1.05 (d, J=7.1 Hz, 2.1H).

Example 164((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 161 substitutingintermediate A-3 with intermediate A-22. MS (ESI): mass calcd. forC₁₉H₂₀ClN₇O₂, 413.1. m/z found, 414.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.45 (s, 1.4H), 8.23 (s, 0.6H), 8.17 (d, J=8.4 Hz, 0.7H), 8.08 (d, J=8.5Hz, 0.3H), 7.93 (s, 1.4H), 7.79 (s, 0.6H), 7.28 (d, J=8.7 Hz, 0.7H),7.16 (d, J=8.5 Hz, 0.3H), 5.26-5.16 (m, 0.7H), 5.14-5.04 (m, 0.7H),4.81-4.68 (m, 0.3H), 4.69-4.60 (m, 0.3H), 4.19-4.09 (m, 0.3H), 3.40-3.27(m, 0.7H), 3.19-3.05 (m, 0.7H), 2.97 (td, J=13.5, 3.3 Hz, 0.3H), 2.63(s, 2.1H), 2.34 (s, 0.9H), 2.11-1.89 (m, 3H), 1.40 (d, J=7.2 Hz, 2.1H),1.37-1.28 (m, 1H), 1.18 (d, J=7.0 Hz, 0.9H).

Example 165(2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 101, steps A and B,and Example 143 step C, substituting intermediate A-29 with intermediateA-25. MS (ESI): mass calcd. for C₂₂H₁₉F₄N₅O₂, 461.1. m/z found, 462.0[M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 9.04 (s, 1H), 8.80-8.75 (m, 2H), 8.63(s, 1H), 8.59 (s, 1H), 8.32-8.23 (m, 1H), 7.56-7.41 (m, 2H), 5.40-5.30(m, 0.5H), 5.29-5.24 (m, 0.7H), 5.24-5.16 (m, 0.5H), 4.87-4.81 (m,0.3H), 4.82-4.72 (m, 0.3H), 3.51-3.38 (m, 0.7H), 3.23-2.84 (m, 1H),2.15-1.89 (m, 3H), 1.43 (d, J=7.1 Hz, 1.5H), 1.39 (d, J=7.3 Hz, 1.5H),1.36-1.27 (m, 1H).

Example 166(2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substituting2-chloro-5-(trifluoromethyl)pyridine with2-chloro-5-(trifluoromethyl)pyrazine, and intermediate A-29 withintermediate A-25. MS (ESI): mass calcd. for C₂₂H₁₉F₄N₅O₂, 461.1. m/zfound, 461.9 [M+H]⁺. Analytical HPLC was obtained on a Agilent 1100Series using a Zorbax SB-C18 column (3.5 μm, 150×4.6 mm), mobile phaseof 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for 3 min,at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=6.34 min at 254nm.

Example 167(3-methyl-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 165 substitutingintermediate A-25 with intermediate A-27. MS (ESI): mass calcd. forC₂₃H₂₂F₃N₅O₂, 457.2. m/z found, 457.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=5.69 min at 254 nm.

Example 168((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 166 substitutingintermediate A-25 with intermediate A-29. MS (ESI): mass calcd. forC₂₂H₂₀F₃N₅O₂, 443.2. m/z found, 443.95 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.64 (s, 0.8H), 8.59 (s, 1.2H), 8.45 (s, 0.8H), 8.35-8.27 (m, 1.2H),8.16-8.11 (m, 1H), 7.58-7.36 (m, 2H), 7.33-7.26 (m, 1.2H)*, 7.10-6.99(m, 0.4H), 6.87 (t, J=7.5 Hz, 0.4H), 5.30 (s, 0.6), 5.24-5.08 (m, 0.5H),4.90-4.82 (m, 0.4H), 4.82-4.74 (m, 0.4H), 4.06-3.92 (m, 0.5H), 3.55-3.33(m, 0.6H), 3.22-3.05 (m, 0.6H), 2.98-2.82 (m, 0.4H), 2.21-1.81 (m, 3H),1.77-1.63 (m, 0.4H), 1.43 (d, J=7.2 Hz, 2.4H), 0.89 (d, J=6.9 Hz, 1.2H).*Peak is partially buried under CDCl₃ peak.

Example 169((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 165 substitutingintermediate A-25 with intermediate A-29. MS (ESI): mass calcd. forC₂₂H₂₀F₃N₅O₂, 443.2. m/z found, 444.0 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=5.63 min at 254 nm.

Example 170(2-(5-fluoropyrimidin-2-yl)-3-methylphenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 165 substitutingintermediate A-25 with intermediate A-38. MS (ESI): mass calcd. forC₂₃H₂₁F₄N₅O₂, 475.2. m/z found, 475.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.30 min at 254 nm.

Example 171(3-methyl-2-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 166 substitutingintermediate A-25 with intermediate A-27. MS (ESI): mass calcd. forC₂₃H₂₂F₃N₅O₂, 457.2. m/z found, 458.0 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.04 min at 254 nm.

Example 172(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 125 substitutingintermediate A-3 with intermediate A-10. MS (ESI): mass calcd. forC₂₁H₂₁F₃N₆O₃, 462.2. m/z found, 463.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.44 (s, 0.4H), 8.29 (s, 1.2H), 8.18 (s, 0.4H), 7.80 (s, 1.2H), 7.78 (s,0.8H), 7.52 (d, J=2.5 Hz, 0.4H), 7.40-7.35 (m, 1.1H), 7.03-6.94 (m,1.1H), 6.36 (dd, J=8.5, 2.5 Hz, 0.4H), 5.27-5.23 (m, 0.4H), 5.22-5.11(m, 0.6H), 4.86-4.82 (m, 0.6H), 4.79-4.69 (m, 0.6H), 4.01-3.92 (m,0.4H), 3.91 (s, 1.8H), 3.77 (s, 1.2H), 3.45-3.30 (m, 0.4H), 2.93-2.77(m, 1H), 2.13-1.85 (m, 3H), 1.70-1.62 (m, 0.4H), 1.44-1.27 (m, 2.4H),0.76 (d, J=7.0 Hz, 1.2H).

Example 173((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(3-phenylpyrazin-2-yl)methanone

The title compound was prepared analogous to Example 125 substitutingintermediate A-3 with intermediate A-33. MS (ESI): mass calcd. forC₂₂H₂₀F₃N₅O₂, 443.2. m/z found, 443.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.07 and 6.18 min at 254 nm.

Example 174(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 161 substituting2,5-dichloropyrimidine with 2-chloro-5-(trifluoromethyl)pyrimidine, andsubstituting intermediate A-3 with intermediate A-10. MS (ESI): masscalcd. for C₂₁H₂₁F₃N₆O₃, 462.2. m/z found, 464.1 [M+H]⁺. ¹H NMR (400MHz, CDCl₃) δ 8.78 (s, 0.3H), 8.75 (s, 0.7H), 8.65 (s, 0.7H), 8.09 (s,0.3H), 7.80 (s, 1H), 7.77 (s, 1H), 7.50 (d, J=2.6 Hz, 0.5H), 7.40 (d,J=8.5 Hz, 0.5H), 7.38 (d, J=2.5 Hz, 0.5H), 7.12 (d, J=8.5 Hz, 0.5H),6.95 (dd, J=8.5, 2.5 Hz, 0.5H), 6.37 (dd, J=8.5, 2.6 Hz, 0.5H),5.25-5.12 (m, 1H), 4.85-4.80 (m, 0.5H), 4.79-4.71 (m, 0.5H), 4.10-4.01(m, 0.5H), 3.89 (s, 2H), 3.77 (s, 1H), 3.43-3.31 (m, 0.5H), 2.92-2.80(m, 1H), 2.07-1.87 (m, 3H), 1.69-1.61 (m, 0.5H), 1.44-1.31 (m, 2H), 0.86(d, J=7.0 Hz, 1.5H).

Example 175(5-fluoro-2-(oxazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 174 substitutingintermediate A-10 with intermediate A-35. MS (ESI): mass calcd. forC₂₁H₁₈F₄N₄O₃, 450.1. m/z found, 452.0 [M+H]⁺. ¹HNMR contains a mixtureof more than two rotamers. Analytical HPLC was obtained on a Agilent1100 Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobilephase of 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for3 min, at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=5.73 minat 254 nm.

Example 176(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 174 substitutingintermediate A-10 with intermediate A-30. MS (ESI): mass calcd. forC₂₀H₂₀F₃N₇O₂, 447.2. m/z found, 448.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.77 (s, 1H), 8.68 (s, 1H), 8.44 (d, J=2.2 Hz, 0.7H), 8.25 (d, J=2.2 Hz,0.3H), 7.87 (s, 1H), 7.83 (s, 1H), 7.71 (d, J=2.2 Hz, 0.5H), 7.66 (d,J=2.2 Hz, 0.5H), 5.28-5.24 (m, 0.5H), 5.24-5.15 (m, 0.5H), 4.89-4.84 (m,0.5H), 4.82-4.70 (m, 0.5H), 3.99-3.90 (m, 0.5H), 3.31-3.22 (m, 0.5H),2.93 (td, J=13.2, 3.1 Hz, 1H), 2.43 (s, 1.8H), 2.14 (s, 1.2H), 2.12-1.86(m, 3H), 1.74-1.64 (m, 0.5H), 1.49-1.37 (m, 2H), 0.92 (d, J=7.0 Hz,1.5H).

Example 177(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 125 substitutingintermediate A-3 with intermediate A-30. MS (ESI): mass calcd. forC₂₀H₂₀F₃N₇O₂, 447.2. m/z found, 448.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.49-8.44 (m, 1H), 8.31 (d, J=1.4 Hz, 0.5H), 8.28 (d, J=1.3 Hz, 0.5H),8.25-8.20 (m, 1H), 7.88 (s, 1H), 7.85 (s, 1H), 7.68-7.64 (m, 0.5H),7.38-7.34 (m, 0.5H), 5.29-5.25 (m, 0.5H), 5.19-5.10 (m, 0.5H), 4.89-4.80(m, 0.5H), 4.81-4.70 (m, 0.5H), 3.94-3.82 (m, 0.5H), 3.33-3.23 (m,0.5H), 2.91 (td, J=13.2, 3.1 Hz, 1H), 2.47 (s, 1.5H), 2.05 (s, 1.5H),2.04-1.77 (m, 3H), 1.49-1.34 (m, 2.5H), 0.81 (d, 1.5H).

Example 178((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(3-phenylpyrazin-2-yl)methanone

The title compound was prepared analogous to Example 174 substitutingintermediate A-10 with intermediate A-33. MS (ESI): mass calcd. forC₂₂H₂₀F₃N₅O₂, 443.2. m/z found, 0-521-4 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃)δ 8.78 (d, J=0.9 Hz, 1.3H), 8.68 (d, J=2.4 Hz, 0.65H), 8.61 (d, J=0.9Hz, 0.7H), 8.55 (d, J=2.4 Hz, 0.65H), 8.50 (d, J=2.4 Hz, 0.35H), 8.09(d, J=2.4 Hz, 0.35H), 7.94-7.76 (series of m, 2H), 7.62-7.43 (series ofm, 3H), 5.30-5.22 (m, 0.65H), 5.19-5.11 (m, 0.65H), 4.89-4.79 (m,0.35H), 4.81-4.69 (m, 0.35H), 4.01-3.90 (m, 0.35H), 3.32-3.15 (m,0.65H), 3.09-2.94 (m, 0.65H), 2.88 (td, J=13.4, 3.3 Hz, 0.35H),2.19-1.82 (m, 3H), 1.39-1.30 (m, 1H), 1.29-1.21 (m, 3H).

Example 179(5-fluoro-2-(oxazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 125 substitutingintermediate A-3 with intermediate A-35. MS (ESI): mass calcd. forC₂₁H₁₈F₄N₄O₃, 450.1. m/z found, 451.0 [M+H]⁺. ¹HNMR contains a mixtureof more than two rotamers. Analytical HPLC was obtained on a Agilent1100 Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobilephase of 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for3 min, at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=6.04 minat 254 nm.

Example 180((2S*,3R*)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 86 substitutingintermediate A-17 with intermediate A-11. MS (ESI): mass calcd. forC₂₀H₂₂ClN₇O, 411.2. m/z found, 412.2 [M+H]⁺. MP=111.1° C.

Example 181((2S*,3R*)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 77 substitutingintermediate A-17 with intermediate A-10. MS (ESI): mass calcd. forC₂₁H₂₃ClN₆O₂, 426.2. m/z found, 427.2 [M+H]⁺. MP=115.4° C.

Example 182((2S*,3R*)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 86 substitutingintermediate A-17 with intermediate A-21. MS (ESI): mass calcd. forC₁₉H₂₁ClN₈O, 412.2. m/z found, 413.1 [M+H]⁺. MP=84.4° C.

Example 183((2S*,3R*)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 30 substitutingintermediate A-4 with intermediate A-21. MP=112.9° C.

Example 184(5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 38 substitutingintermediate A-4 with intermediate A-14. MP=148.5° C.

Example 185 ((2S*,3R*)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 30 substitutingintermediate A-4 with intermediate A-10. MP=99.5° C.

Example 186((2S*,3R*)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 86 substitutingintermediate A-17 with intermediate A-10. MS (ESI): mass calcd. forC₂₀H₂₂ClN₇O₂, 427.2. m/z found, 428.1 [M+H]⁺. MP=112.8° C.

Example 187((2S*,3R*)-3-(benzo[d]oxazol-2-yl(methyl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Step D:((2S*,3R*)-3-(benzo[d]oxazol-2-yl(methyl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

To a solution of compound of Example 73 (20 mg, 0.047 mmol) in DMF (0.47mL) was added sodium tert-butoxide (6 mg, 0.06 mmol) followed byiodomethane (3 μL, 0.049 mmol). The reaction mixture was stirred at roomtemperature for 16 h. Solvent was evaporated and purification via prepHPLC gave the title compound (14 mg, 69%). MS (ESI): mass calcd. forC₂₃H₂₅N₇O₂, 431.2. m/z found, 432.0 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.47 (s, 1H), 8.18 (s, 1H), 7.90-7.75 (m, 2H), 7.37 (d, J=7.7 Hz, 1H),7.27 (d, J=8.9 Hz, 1H), 7.19-7.14 (m, 1H), 7.04-6.98 (m, 1H), 5.05-4.92(m, 1H), 4.44-4.32 (m, 1H), 3.43-3.33 (m, 4H), 3.34-3.21 (m, 1H), 2.48(s, 3H), 2.18-2.04 (m, 1H), 1.99-1.76 (m, 2H), 1.62-1.53 (m, 1H), 1.47(d, J=6.8 Hz, 3H).

Example 188(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S*,3R*)-2-methyl-3-(methyl(5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-8, followed by step D of Example187. MS (ESI): mass calcd. for C₂₂H₂₄F₃N₇O, 459.2. m/z found, 460.0[M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ 8.38 (s, 1H), 7.90-7.85 (m, 2H), 7.70(d, J=7.7 Hz, 1H), 7.64 (dd, J=9.1, 2.6 Hz, 1H), 7.30 (d, J=7.5 Hz, 1H),6.54 (d, J=9.0 Hz, 1H), 4.92-4.74 (m, 2H), 3.29-3.19 (m, 1H), 3.09 (s,3H), 3.02-2.89 (m, 1H), 2.70 (s, 3H), 1.92-1.74 (m, 2H), 1.72-1.62 (m,1H), 1.59-1.43 (m, 1H), 1.39-1.29 (m, 3H).

Example 189(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-28. MS (ESI): mass calcd. forC₂₃H₂₁F₄N₅O, 459.2. m/z found, 460.0 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.94 (d, J=4.9 Hz, 2H), 8.32-8.15 (m, 2H), 7.53-7.33 (m, 3H), 7.13 (d,J=7.2 Hz, 1H), 6.24 (d, J=8.8 Hz, 1H), 4.56 (d, J=13.5 Hz, 1H),4.25-4.12 (m, 1H), 4.04 (s, 1H), 2.97-2.80 (m, 1H), 2.11-1.90 (m, 2H),1.77-1.62 (m, 2H), 1.52 (d, J=6.9 Hz, 3H). 1H buried under CDCl₃ peak.

Example 190(4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-36. MS (ESI): mass calcd. forC₂₂H₂₁F₄N₅O₂, 463.2. m/z found, 464.0 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using an Inertsil ODS-3 column (3 μm,50×3 mm), mobile phase of 5-99% ACN in 0.05% TFA over 1.6 min and thenhold at 99% ACN for 0.4 min, at a flow rate of 2.2 mL/min(Temperature=50° C.). R_(t)=1.37 min (major rotamer) at 254 nm.

Example 191(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-37. MS (ESI): mass calcd. forC₂₃H₂₁F₄N₅O, 459.2. m/z found, 460.0 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.94 (d, J=4.9 Hz, 2H), 8.21 (s, 1H), 7.89 (dd, J=9.5, 2.7 Hz, 1H),7.87-7.79 (m, 1H), 7.40 (t, J=4.9 Hz, 1H), 7.36-7.28 (m, 2H), 7.23-7.16(m, 2H), 6.01-5.84 (m, 1H), 4.77-4.64 (m, 1H), 4.24-4.13 (m, 1H),3.01-2.90 (m, 1H), 2.08-1.77 (m, 4H), 1.52 (d, J=6.9 Hz, 3H).

Example 192(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-1. MS (ESI): mass calcd. forC₂₁H₂₀F₄N₆O, 448.2. m/z found, 448.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using an Inertsil ODS-3 column (3 μmm,50×3 mm), mobile phase of 5-99% ACN in 0.05% TFA over 1.6 min and thenhold at 99% ACN for 0.4 min, at a flow rate of 2.2 mL/min(Temperature=50° C.). R_(t)=1.34 min (major rotamer) at 254 nm.

Example 193(2-chloro-6-methoxypyridin-3-yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-32. MS (ESI): mass calcd. forC₁₉H₂₀ClF₃N₄O₂, 428.1. m/z found, 428.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using an Inertsil ODS-3 column (3 μm,50×3 mm), mobile phase of 5-99% ACN in 0.05% TFA over 1.6 min and thenhold at 99% ACN for 0.4 min, at a flow rate of 2.2 mL/min(Temperature=50° C.). R_(t)=1.34 min (major rotamer) at 254 nm.

Example 194(3-ethoxy-6-methylpyridin-2-yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-12. MS (ESI): mass calcd. forC₂₁H₂₅F₃N₄O₂, 422.2. m/z found, 423.0 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.35-8.19 (m, 1H), 7.58-7.47 (m, 1H), 7.20-7.02 (m, 2H), 6.59-6.44 (m,1H), 6.37 (s, 1H), 4.78-4.61 (m, 1H), 4.14-3.97 (m, 2H), 3.88 (s, 1H),3.78-3.63 (m, 1H), 2.97 (td, J=13.2, 3.3 Hz, 1H), 2.52-2.37 (m, 3H),2.05-1.95 (m, 1H), 1.94-1.73 (m, 2H), 1.64-1.55 (m, 1H), 1.49-1.38 (m,3H), 1.33 (d, J=6.9 Hz, 3H).

Example 195((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Step A: (2S*,3R*)-tert-butyl2-methyl-3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)piperidine-1-carboxylate

In a microwave vial was dissolved intermediate B-4 (58 mg, 0.27 mmol) inACN (0.7 mL). 2-Chloro-5-(trifluoromethyl)-1,3,4-thiadiazole (0.59 mg,0.30 mmol) was added followed by DIPEA (0.12 mL, 0.68 mmol). Themicrowave vial was capped and the reaction mixture was heated at 120° C.for 30 minutes using microwave and was then diluted with EtOAc. Theorganic phase was washed with a saturated solution of NaHCO₃, NaCl,dried over MgSO₄, filtered and evaporated. Purification via silica gelchromatography (0-40% EtOAc in hexanes) gave the title compound (87 mg,88%). MS (ESI): mass calcd. for C₁₄H₂₁F₃N₄O₂S, 366.1. m/z found, 367.0[M+H]⁺. Analytical HPLC was obtained on a Agilent 1100 Series using anInertsil ODS-3 column (3 μm, 50×3 mm), mobile phase of 5-99% ACN in0.05% TFA over 1.6 min and then hold at 99% ACN for 0.4 min, at a flowrate of 2.2 mL/min (Temperature=50° C.). R_(t)=1.409 min at 254 nm.

Step B and C:((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 1, steps B and C,substituting intermediate A-1 with intermediate A-21. MS (ESI): masscalcd. for C₁₈H₁₉F₃N₈OS, 452.1. m/z found, 452.9 [M+H]⁺. ¹H NMR (500MHz, CDCl₃) δ 8.45 (s, 1H), 8.27-8.18 (m, 1H), 8.11 (s, 1H), 7.96 (s,2H), 4.68-4.60 (m, 1H), 4.08-4.00 (m, 1H), 3.99-3.92 (m, 1H), 3.04 (td,J=13.4, 3.3 Hz, 1H), 2.48 (s, 3H), 2.21 (d, J=14.0 Hz, 1H), 2.09-1.93(m, 2H), 1.92-1.79 (m, 1H), 1.56-1.47 (m, 3H).

Example 196(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 195 substitutingintermediate A-21 with intermediate A-8. MS (ESI): mass calcd. forC₁₈H₁₉F₃N₈OS, 452.1. m/z found, 452.9 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.33 (d, J=8.1 Hz, 0.6H), 8.30 (s, 0.8H), 8.04 (s, 1.2H), 7.61 (d, J=7.8Hz, 0.6H), 7.57 (d, J=7.7 Hz, 0.4H), 7.33 (d, J=7.8 Hz, 0.6H), 7.28 (d,J=8.0 Hz, 0.4H), 7.02 (d, J=8.5 Hz, 0.4H), 5.38-5.29 (m, 0.4H),4.74-4.62 (m, 0.6H), 4.20-4.11 (m, 0.6H), 4.11-4.05 (m, 0.6H), 3.83-3.69(m, 0.4H), 3.60-3.42 (m, 0.4H), 3.31 (td, J=13.5, 3.3 Hz, 0.4H), 2.96(td, J=13.4, 3.3 Hz, 0.6H), 2.71 (s, 1.2H), 2.70 (s, 1.8H), 2.23-1.67(series of m, 4H), 1.52 (d, J=6.9 Hz, 1.8H), 1.45 (d, J=7.1 Hz, 1.2H).

Example 197(4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-36. MS (ESI): mass calcd. forC₂₁H₂₀F₄N₆O₂, 464.2. m/z found, 464.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using an Inertsil ODS-3 column (3 μm,50×3 mm), mobile phase of 5-99% ACN in 0.05% TFA over 1.6 min and thenhold at 99% ACN for 0.4 min, at a flow rate of 2.2 mL/min(Temperature=50° C.). R_(t)=1.42 min (major rotamer) at 254 nm.

Example 198(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-1. MS (ESI): mass calcd. forC₂₀H₁₉F₄N₇O, 449.2. m/z found, 449.9 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using an Inertsil ODS-3 column (3 μm,50×3 mm), mobile phase of 5-99% ACN in 0.05% TFA over 1.6 min and thenhold at 99% ACN for 0.4 min, at a flow rate of 2.2 mL/min(Temperature=50° C.). R_(t)=1.43 min (major rotamer) at 254 nm.

Example 199(3-ethoxy-6-methylpyridin-2-yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-12. MS (ESI): mass calcd. forC₂₀H₂₄F₃N₅O₂, 423.2. m/z found, 424.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.50-8.42 (m, 2H), 8.39 (s, 1H), 7.20-7.03 (m, 2H), 4.76-4.62 (m, 1H),4.18-4.02 (m, 2H), 3.90-3.81 (m, 1H), 3.79-3.64 (m, 1H), 2.97 (td,J=13.2, 3.3 Hz, 1H), 2.49 (s, 3H), 2.11-2.01 (m, 1H), 1.99-1.74 (m, 3H),1.67-1.56 (m, 1H), 1.46-1.42 (m, 2H), 1.33 (d, J=7.0 Hz, 3H).

Example 200(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-28. MS (ESI): mass calcd. forC₂₂H₂₀F₄N₆O, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ9.21 (d, J=7.0 Hz, 1H), 9.03 (d, J=5.0 Hz, 2H), 8.43 (s, 1H), 8.37 (s,1H), 7.50-7.41 (m, 1H), 7.37 (t, J=5.0 Hz, 1H), 7.15 (d, J=7.6 Hz, 1H),4.68-4.54 (m, 1H), 4.25-4.13 (m, 1H), 4.09-3.94 (m, 1H), 2.90 (td,J=13.4, 3.3 Hz, 1H), 2.16-2.04 (m, 1H), 1.98-1.86 (m, 1H), 1.81-1.56 (m,2H), 1.52 (d, J=6.9 Hz, 3H). 1H buried under CDCl₃ peak.

Example 201(2-chloro-6-methoxypyridin-3-yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-32. MS (ESI): mass calcd. forC₁₈H₁₉ClF₃N₅O₂, 429.1. m/z found, 429.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃,multiple rotamers present, only major rotamer reported) δ 8.49 (broad s,2H), 7.63 (d, J=8.2 Hz, 1H), 7.07 (d, J=8.3 Hz, 1H), 6.10 (d, J=8.3 Hz,1H), 5.71 (d, J=6.3 Hz, 1H), 5.18-4.98 (m, 1H), 4.69 (d, J=13.5 Hz, 1H),3.87 (s, 3H), 3.48-3.26 (m, 1H), 1.99-1.72 (m, 4H), 1.48-1.39 (m, 3H).

Example 202(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-37. MS (ESI): mass calcd. forC₂₂H₂₀F₄N₆O, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ9.18 (d, J=4.9 Hz, 0.6H), 8.98 (d, J=4.9 Hz, 1.4H), 8.82 (d, J=7.4 Hz,0.7H), 8.52 (s, 0.6H), 8.44-8.35 (m, 0.7H), 8.30-8.24 (m, 0.7H),8.21-8.16 (m, 0.3H), 7.89 (dd, J=9.6, 2.6 Hz, 0.7H), 7.37-7.29 (m,1.3H), 7.21-7.15 (m, 1H), 5.30-5.21 (m, 0.3H), 4.72 (d, J=13.5 Hz,0.7H), 4.34-4.23 (m, 0.3H), 4.23-4.12 (m, 0.7H), 4.08-3.98 (m, 0.7H),3.54-3.44 (m, 0.3H), 3.23 (td, J=13.3, 3.2 Hz, 0.3H), 2.95 (td, J=13.3,3.1 Hz, 0.7H), 2.14-1.63 (m, 4H), 1.52 (d, J=6.9 Hz, 2.1H), 1.44 (d,J=7.1 Hz, 0.9H).

Example 203(4-fluoro-2-(oxazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-34. MS (ESI): mass calcd. forC₂₂H₂₀F₄N₄O₂, 448.2. m/z found, 448.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.36 (s, 0.5H), 8.19 (s, 0.5H), 7.89-7.86 (m, 0.5H), 7.85-7.80 (m,0.5H), 7.79 (dd, J=9.6, 2.5 Hz, 0.5H), 7.61 (dd, J=9.2, 2.5 Hz, 0.5H),7.57 (dd, J=8.9, 2.5 Hz, 0.5H), 7.41-7.27 (m, 1.5H), 7.25-7.14 (m, 2H),6.98-6.88 (m, 1H), 6.45 (d, J=9.1 Hz, 0.5H), 5.97 (d, J=8.8 Hz, 0.5H),5.34-5.19 (m, 0.5H), 4.83-4.70 (m, 0.5H), 4.18-4.05 (m, 1H), 3.93-3.82(m, 0.5H), 3.36-3.27 (m, 0.5H), 3.20 (td, J=13.3, 3.2 Hz, 0.5H), 2.97(td, J=13.3, 12.7, 3.3 Hz, 0.5H), 1.99-1.65 (m, 4H), 1.46-1.39 (m, 3H).

Example 204((2S*,3R*)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(3-fluoro-2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 73 substitutingintermediate A-21 with intermediate A-28. MS (ESI): mass calcd. forC₂₄H₂₂FN₅O₂, 431.2. m/z found, 432.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ9.17 (d, J=7.3 Hz, 1H), 9.01 (d, J=5.0 Hz, 2H), 7.54-7.41 (m, 2H),7.33-7.26 (m, 2H), 7.19-7.07 (m, 3H), 6.96 (td, J=7.8, 1.3 Hz, 1H),4.64-4.52 (m, 1H), 4.26-4.17 (m, 1H), 4.01-3.93 (m, 1H), 2.91 (td,J=13.4, 3.2 Hz, 1H), 2.32-2.19 (m, 1H), 2.05-1.91 (m, 1H), 1.84-1.57 (m,2H), 1.52 (d, J=6.9 Hz, 3H).

Example 205(4-fluoro-2-(oxazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 69 substitutingintermediate A-21 with intermediate A-34. MS (ESI): mass calcd. forC₂₁H₁₉F₄N₅O₂, 449.1. m/z found, 449.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.55-8.47 (m, 2H), 8.07 (d, J=0.8 Hz, 1H), 7.97 (d, J=8.7 Hz, 1H),7.84-7.75 (m, 2H), 7.25-7.13 (m, 2H), 5.37-5.23 (m, 1H), 4.42-4.28 (m,1H), 3.45-3.30 (m, 1H), 3.21 (td, J=13.4, 3.3 Hz, 1H), 2.06-1.64 (m,4H), 1.43 (d, J=7.3 Hz, 3H).

Example 206((2S*,3R*)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1-yl)(4-fluoro-2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 73 substitutingintermediate A-21 with intermediate A-37. MS (ESI): mass calcd. forC₂₄H₂₂FN₅O₂, 431.2. m/z found, 432.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.99 (d, J=4.9 Hz, 2H), 8.80 (d, J=7.6 Hz, 1H), 7.88 (dd, J=9.5, 2.7 Hz,1H), 7.44 (t, J=4.9 Hz, 1H), 7.33-7.27 (m, 2H), 7.24-7.16 (m, 1H),7.14-7.02 (m, 2H), 6.97-6.91 (m, 1H), 4.79-4.63 (m, 1H), 4.31-4.19 (m,1H), 4.03-3.90 (m, 1H), 2.97 (td, J=13.4, 3.1 Hz, 1H), 2.22 (d, J=14.0Hz, 1H), 2.06-1.95 (m, 1H), 1.94-1.78 (m, 1H), 1.74-1.66 (m, 1H), 1.53(d, J=6.9 Hz, 3H).

Example 207(3-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 38 substitutingintermediate A-4 with intermediate A-28. MS (ESI): mass calcd. forC₂₂H₂₀F₄N₆O, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.92 (d, J=5.0 Hz, 2H), 8.69 (d, J=7.6 Hz, 1H), 8.22 (s, 1H), 7.73 (s,1H), 7.52-7.44 (m, 1H), 7.42 (t, J=5.0 Hz, 1H), 7.29 (d, J=9.4 Hz, 1H),7.13 (d, J=7.6 Hz, 1H), 4.63-4.52 (m, 1H), 4.23-4.14 (m, 1H), 4.13-4.00(m, 1H), 2.99-2.81 (m, 1H), 2.10-1.88 (m, 2H), 1.76-1.63 (m, 2H), 1.53(d, J=6.9 Hz, 3H).

Example 208(4-fluoro-2-(oxazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 38 substitutingintermediate A-4 with intermediate A-34. MS (ESI): mass calcd. forC₂₁H₁₉F₄N₅O₂, 449.1. m/z found, 449.9 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.34 (s, 0.4H), 8.18 (s, 0.6H), 8.04-7.95 (m, 1H), 7.87 (s, 0.6H),7.83-7.76 (m, 0.8H), 7.62 (dd, J=9.0, 2.6 Hz, 0.6H), 7.54-7.47 (m,0.4H), 7.41 (s, 0.6H), 7.33-7.28 (m, 0.6H), 7.25-7.17 (m, 1.4H),5.33-5.18 (m, 0.4H), 4.84-4.68 (m, 0.6H), 4.37-4.21 (m, 0.4H), 4.16-4.04(m, 0.6H), 3.96-3.83 (m, 0.6H), 3.43-3.30 (m, 0.4H), 3.24 (td, J=13.4,3.2 Hz, 0.4H), 2.98 (td, J=13.2, 3.1 Hz, 0.6H), 2.05-1.67 (m, 4H),1.51-1.41 m, 3H).

Example 209(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 38 substitutingintermediate A-4 with intermediate A-37. MS (ESI): mass calcd. forC₂₂H₂₀F₄N₆O, 460.2. m/z found, 460.9 [M+H]⁺. ¹H NMR (500 MHz, CDCl₃) δ8.91 (d, J=4.9 Hz, 2H), 8.31 (d, J=7.9 Hz, 1H), 8.20 (s, 1H), 7.90 (dd,J=9.4, 2.6 Hz, 1H), 7.40 (t, J=4.9 Hz, 1H), 7.37 (s, 1H), 7.30 (dd,J=8.5, 5.3 Hz, 1H), 7.20 (td, J=8.1, 2.7 Hz, 1H), 4.79-4.66 (m, 1H),4.25-4.16 (m, 1H), 4.15-4.01 (m, 1H), 2.97 (td, J=13.3, 3.2 Hz, 1H),2.09-1.92 (m, 2H), 1.88-1.63 (m, 2H), 1.54 (d, J=6.9 Hz, 3H).

Example 210((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-38. MS (ESI): mass calcd. forC₂₂H₂₀F₃N₅O₂, 443.2. m/z found, 444.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.82-8.75 (m, 2H), 8.72 (dd, J=4.8, 1.7 Hz, 0.5H), 8.64 (dd, J=8.0, 1.7Hz, 0.5H), 8.50 (dd, J=8.0, 1.7 Hz, 0.4H), 8.44-8.40 (m, 0.6H),8.09-8.03 (m, 0.4H), 8.00 (dd, J=4.8, 1.7 Hz, 0.6H), 7.76 (dd, J=8.8,2.6 Hz, 0.6H), 7.69 (dd, J=8.8, 2.6 Hz, 0.4H), 7.44 (dd, J=8.0, 4.7 Hz,0.5H), 7.29-7.14 (m, 1.5H), 6.92 (d, J=8.8 Hz, 0.6H), 6.69 (d, J=8.7 Hz,0.4H), 5.35-5.30 (m, 0.6H), 5.17-5.08 (m, 0.6H), 4.88-4.80 (m, 0.4H),4.80-4.70 (m, 0.4H), 4.10-3.97 (m, 0.4H), 3.47-3.34 (m, 0.6H), 3.21 (td,J=12.9, 3.0 Hz, 0.6H), 2.98 (td, J=13.3, 3.2 Hz, 0.4H), 2.25-1.91 (m,3H), 1.76-1.48 (m, 1H), 1.44 (d, J=7.2 Hz, 1.8H), 1.14 (d, J=7.0 Hz,1.2H).

Example 211(5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-39. MS (ESI): mass calcd. forC₂₃H₂₂F₃N₅O₂, 457.2. m/z found, 458.2 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.87 (d, J=4.8 Hz, 1H), 8.84 (d, J=4.8 Hz, 1H), 8.71-8.67 (m, 0.5H),8.50-8.46 (m, 0.5H), 8.45-8.41 (m, 0.5H), 8.24-8.15 (m, 0.5H), 7.87-7.81(m, 0.5H), 7.79 (dd, J=8.6, 2.6 Hz, 0.5H), 7.59-7.53 (m, 0.5H),7.32-7.24 (m, 1.5H), 6.89-6.82 (m, 1H), 5.36-5.31 (m, 0.5H), 5.17-5.08(m, 0.5H), 4.92-4.85 (m, 0.5H), 4.81-4.74 (m, 0.5H), 4.08-4.01 (m,0.5H), 3.45-3.26 (m, 0.5H), 3.16-3.01 (m, 0.5H), 2.97-2.85 (m, 0.5H),2.47 (s, 1.5H), 2.15-1.80 (m, 4.5H), 1.76-1.60 (m, 0.5H), 1.52-1.35 (m,2H), 0.88 (d, J=7.0 Hz, 1.5H).

Example 212(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-40. MS (ESI): mass calcd. forC₂₃H₂₀F₄N₄O₂, 460.2. m/z found, 461.0 [M+H]⁺. ¹HNMR contains a mixtureof more than two rotamers. Analytical HPLC was obtained on a Agilent1100 Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobilephase of 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for3 min, at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=6.79 minat 254 nm.

Example 213((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-19. MS (ESI): mass calcd. forC₂₃H₂₂F₃N₅O₂, 457.2. m/z found, 457.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.76 (d, J=3.4 Hz, 1H), 8.75 (d, J=3.5 Hz, 1H), 8.53 (d, J=8.1 Hz,0.5H), 8.45-8.38 (m, 1H), 8.16-8.09 (m, 0.5H), 7.78 (dd, J=8.8, 2.6 Hz,0.5H), 7.62 (dd, J=8.7, 2.5 Hz, 0.5H), 7.30-7.27 (m, 0.5H), 7.23-7.16(m, 1H), 7.10 (d, J=8.2 Hz, 0.5H), 6.94 (d, J=8.7 Hz, 0.5H), 6.54 (d,J=8.7 Hz, 0.5H), 5.33-5.28 (m, 0.5H), 5.19-5.08 (m, 0.5H), 4.87-4.83 (m,0.5H), 4.82-4.76 (m, 0.5H), 4.09-4.00 (m, 0.5H), 3.41-3.31 (m, 0.5H),3.14 (td, J=13.0, 3.0 Hz, 0.5H), 2.96 (td, J=13.3, 3.2 Hz, 0.5H), 2.65(s, 1.5H), 2.27 (s, 1.5H), 2.21-1.90 (m, 3H), 1.71-1.62 (m, 0.5H), 1.44(d, J=7.1 Hz, 1.5H), 1.41-1.32 (m, 0.5H), 1.11 (d, J=7.0 Hz, 1.5H).

Example 214((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

The title compound was prepared analogous to Example 147 substituting2-chloro-5-(trifluoromethyl)pyridine with 2-bromo-5-chloropyridine andsubstituting A-1 with A-41. MS (ESI): mass calcd. for C₂₀H₂₁ClN₆O₂,412.1. m/z found, 412.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.45 (d, J=2.3Hz, 0.4H), 8.28 (d, J=2.2 Hz, 0.6H), 8.07 (d, J=2.8 Hz, 0.4H), 7.86 (s,1.2H), 7.85-7.82 (m, 1.4H), 7.66-7.63 (m, 0.4H), 7.57 (dd, J=8.8, 2.7Hz, 0.6H), 7.53 (dd, J=8.8, 2.6 Hz, 0.4H), 7.32-7.29 (m, 0.6H),6.76-6.68 (m, 1H), 5.19-5.14 (m, 0.4H), 5.13-5.04 (m, 0.4H), 4.77-4.67(m, 1.2H), 4.05-3.93 (m, 0.6H), 3.30-3.19 (m, 0.4H), 2.96-2.83 (m, 1H),2.44 (s, 1.2H), 2.13-1.77 (m, 4.8H), 1.67-1.60 (m, 0.6H), 1.36 (d, J=7.1Hz, 1.2H), 1.33-1.18 (m, 0.4H), 0.82 (d, J=7.0 Hz, 1.8H).

Example 215((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 214 substitutingintermediate A-41 with intermediate A-22. MS (ESI): mass calcd. forC₂₀H₂₁ClN₆O₂, 412.1. m/z found, 412.9 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.15 (d, J=8.3 Hz, 0.5H), 8.08 (d, J=8.4 Hz, 0.5H), 8.05 (d, J=2.7 Hz,0.5H), 7.80 (s, 1H), 7.79 (s, 1H), 7.78 (d, J=2.7 Hz, 0.5H), 7.51 (dd,J=8.8, 2.7 Hz, 0.5H), 7.42 (dd, J=8.8, 2.7 Hz, 0.5H), 7.29 (d, J=8.4 Hz,0.5H), 7.14 (d, J=8.5 Hz, 0.5H), 6.79 (d, J=8.8 Hz, 0.5H), 6.54 (d,J=8.8 Hz, 0.5H), 5.17-5.13 (m, 0.5H), 5.11-5.03 (m, 0.5H), 4.80-4.68 (m,1H), 4.03-3.93 (m, 0.5H), 3.34-3.27 (m, 0.5H), 3.06 (td, J=13.0, 3.1 Hz,0.5H), 2.92 (td, J=13.4, 3.3 Hz, 0.5H), 2.64 (s, 1.5H), 2.31 (s, 1.5H),2.24-1.77 (m, 3H), 1.68-1.54 (m, 0.5H), 1.42-1.28 (m, 2H), 1.02 (d,J=6.9 Hz, 1.5H).

Example 216((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 143 substituting2-chloro-5-(trifluoromethyl)pyridine with 2-bromo-5-chloropyridine, andsubstituting A-29 with A-20. MS (ESI): mass calcd. for C₂₂H₂₂ClN₅O₂,423.1. m/z found, 424.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.81-8.74 (m,2H), 8.57-8.51 (m, 0.6H), 8.46-8.39 (m, 0.6H), 8.33-8.28 (m, 0.4H),8.10-8.04 (m, 0.6H), 7.94-7.88 (m, 0.4H), 7.75-7.68 (m, 0.4H), 7.51 (dd,J=8.8, 2.7 Hz, 0.6H), 7.45 (dd, J=8.8, 2.7 Hz, 0.4H), 7.24-7.18 (m, 1H),6.79 (d, J=8.8 Hz, 0.6H), 6.55 (d, J=8.8 Hz, 0.4H), 5.23-5.14 (m, 0.6H),5.12-5.03 (m, 0.6H), 4.79-4.71 (m, 0.4H), 4.69-4.66 (m, 0.4H), 4.01-3.90(m, 0.4H), 3.43-3.34 (m, 0.6H), 3.17 (td, J=12.9, 3.0 Hz, 0.6H), 2.93(td, J=13.3, 3.2 Hz, 0.4H), 2.44 (s, 1.8H), 2.32 (s, 1.2H), 2.18-1.81(m, 3H), 1.68-1.57 (m, 0.6H), 1.41 (d, J=7.1 Hz, 1.8H), 1.39-1.32 (m,0.4H), 1.07 (d, J=7.0 Hz, 1.2H).

Example 217((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)methanone

The title compound was prepared analogous to Example 216 substitutingA-20 with A-19. MS (ESI): mass calcd. for C₂₂H₂₂ClN₅O₂, 423.1. m/zfound, 424.2 [M+H]⁺. Analytical HPLC was obtained on a Agilent 1100Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobile phaseof 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for 3 min,at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=4.90 min at 254nm.

Example 218((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 216 substitutingA-20 with A-38. MS (ESI): mass calcd. for C₂₁H₂₀ClN₅O₂, 409.1. m/zfound, 410.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.81-8.76 (m, 2.4H), 8.73(dd, J=4.8, 1.7 Hz, 0.6H), 8.64 (dd, J=8.0, 1.7 Hz, 0.6H), 8.53 (dd,J=8.0, 1.7 Hz, 0.4H), 8.14 (dd, J=4.7, 1.7 Hz, 0.4H), 8.07 (d, J=2.7 Hz,0.6H), 7.73 (d, J=2.8 Hz, 0.4H), 7.52 (dd, J=8.8, 2.7 Hz, 0.6H),7.49-7.39 (m, 1H), 7.25-7.19 (m, 1H), 6.80 (d, J=8.7 Hz, 0.6H), 6.52 (d,J=8.9 Hz, 0.4H), 5.24-5.17 (m, 0.6H), 5.15-5.03 (m, 0.6H), 4.80-4.70 (m,0.8H), 4.02-3.92 (m, 0.4H), 3.44-3.31 (m, 0.6H), 3.20 (td, J=13.0, 3.1Hz, 0.6H), 2.97 (td, J=13.3, 3.2 Hz, 0.4H), 2.25-1.57 (m, 3.4H),1.47-1.34 (m, 2.4H), 1.13 (d, J=7.1 Hz, 1.2H).

Example 219(3-ethoxy-6-methylpyridin-2-yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

The title compound was prepared analogous to Example 143 substitutingintermediate A-29 with intermediate A-12. MS (ESI): mass calcd. forC₂₁H₂₄F₃N₃O₃, 423.2. m/z found, 424.0 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ8.45-8.40 (m, 0.4H), 8.16-8.04 (m, 0.6H), 7.85-7.67 (m, 1H), 7.16-7.03(m, 1H), 6.97-6.83 (m, 2H), 5.32-5.25 (m, 0.4H), 5.23-5.12 (m, 0.4H),4.90-4.83 (m, 0.6H), 4.81-4.69 (m, 0.6H), 4.02-3.80 (m, 2.6H), 3.34-3.17(m, 0.8H), 2.97 (td, J=13.4, 12.9, 3.0 Hz, 0.6H), 2.51 (s, 1.2H), 2.16(s, 1.8H), 2.14-1.89 (m, 3H), 1.69-1.61 (m, 0.4H), 1.43-1.32 (m, 6H).*0.6H buried under solvent peak.

Example 220((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(pyrimidin-2-yl)phenyl)methanone

The title compound was prepared analogous to Example 143 substituting2-chloro-5-(trifluoromethyl)pyridine with2-chloro-3-fluoro-5-(trifluoromethyl)pyridine, and substitutingintermediate A-29 with intermediate A-27. MS (ESI): mass calcd. forC₂₄H₂₂F₄N₄O₂, 474.2. m/z found, 475.1 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a Zorbax SB-C18 column (3.5 μm,150×4.6 mm), mobile phase of 5-99% ACN in 0.05% TFA over 7 min and thenhold at 99% ACN for 3 min, at a flow rate of 2 mL/min (Temperature=50°C.). R_(t)=6.60 min at 254 nm.

Example 221(5-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 220 substituting intermediate A-27 withintermediate A-7. MS (ESI): mass calcd. for C₂₃H₁₉F₅N₄O₂, 478.1. m/zfound, 479.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.76 (d, J=4.8 Hz, 1H),8.72 (d, J=4.8 Hz, 1H), 8.40 (dd, J=8.8, 5.6 Hz, 0.5H), 8.26-8.20 (m,1H), 7.99-7.95 (m, 0.5H), 7.65-7.57 (m, 0.5H), 7.56 (dd, J=9.5, 2.1 Hz,0.5H), 7.23-7.15 (m, 1.5H), 7.12 (dd, J=8.5, 2.7 Hz, 0.5H), 7.04-6.94(m, 1H), 5.42-5.37 (m, 0.5H), 5.19-5.09 (m, 0.5H), 5.04-4.94 (m, 0.5H),4.82-4.72 (m, 0.5H), 4.07-3.96 (m, 0.5H), 3.41-3.33 (m, 0.5H), 3.18-3.06(m, 0.5H), 2.99-2.84 (m, 0.5H), 2.15-1.89 (m, 3H), 1.70-1.63 (m, 0.5H),1.47-1.37 (m, 2H), 0.93 (d, J=7.0 Hz, 1.5H).

Example 222(4-fluoro-2-(pyrimidin-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 220 substituting intermediate A-27 withintermediate A-37. MS (ESI): mass calcd. C₂₃H₁₉F₅N₄O₂, 478.1. m/z found,479.1 [M+H]⁺. Analytical HPLC was obtained on a Agilent 1100 Seriesusing a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobile phase of 5-99%ACN in 0.05% TFA over 7 min and then hold at 99% ACN for 3 min, at aflow rate of 2 mL/min (Temperature=50° C.). R_(t)=6.72 min at 254 nm.

Example 223((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Prepared analogous to Example 220 substituting intermediate A-27 withintermediate A-19. MS (ESI): mass calcd. for C₂₃H₂₁F₄N₅O₂, 475.2. m/zfound, 476.1 [M+H]⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.77 (d, J=4.8 Hz, 1H),8.72 (d, J=4.9 Hz, 1H), 8.49 (d, J=8.1 Hz, 0.5H), 8.39 (d, J=8.1 Hz,0.5H), 8.25-8.16 (m, 0.5H), 7.90-7.81 (m, 0.5H), 7.53 (dd, J=9.7, 2.1Hz, 0.5H), 7.32-7.22 (m, 1H), 7.21-7.17 (m, 0.5H), 7.16-7.10 (m, 1H),5.39-5.32 (m, 0.5H), 5.25-5.16 (m, 0.5H), 4.87-4.76 (m, 1H), 4.23-4.15(m, 0.5H), 3.43-3.31 (m, 0.5H), 3.25-3.10 (m, 0.5H), 2.99 (td, J=13.3,3.2 Hz, 0.5H), 2.62 (s, 1.7H), 2.35 (s, 1.3H), 2.13-1.96 (m, 3H),1.76-1.66 (m, 0.5H), 1.45 (d, J=7.2 Hz, 1.7H), 1.43-1.37 (m, 0.5H), 1.24(d, J=7.0 Hz, 1.3H).

Example 224(2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Prepared analogous to Example 220 substituting intermediate A-27 withintermediate A-4. MS (ESI): mass calcd. for C₂₁H₁₉F₄N₅O₂, 449.2. m/zfound, 450.1 [M+H]⁺. Analytical HPLC was obtained on a Agilent 1100Series using a Zorbax SB-C18 column (3.5 μm, 150×4 6 mm), mobile phaseof 5-99% ACN in 0.05% TFA over 7 min and then hold at 99% ACN for 3 min,at a flow rate of 2 mL/min (Temperature=50° C.). R_(t)=6.61 min at 254nm.

Example 225(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 226((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 227(3-fluoro-2-(oxazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone

Example 228((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 229((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)methanone

Example 230(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 231((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 232(3-fluoro-2-(oxazol-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone

Example 233((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 234((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(4-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 235((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 236((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 237((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Example 238((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

Example 239((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(pyrimidin-2-yl)phenyl)methanone

Example 240((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 241((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 242((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 243((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)methanone

Example 244((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 245((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 246((2S,3R)-3-((3-chloro-5-(trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(oxazol-2-yl)phenyl)methanone

Example 247((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(oxazol-2-yl)phenyl)methanone

Example 248((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 249((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 250((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

Example 251((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(pyrimidin-2-yl)phenyl)methanone

Example 252((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 253((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 254((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone

Example 255((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone

Example 256((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(pyrimidin-2-yl)phenyl)methanone

Example 257((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl-2-(pyrimidin-2-yl)phenyl)methanone

Example 258((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 259((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 260((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 261((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)methanone

Example 262((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(5-fluoropyrimidin-2-yl)phenyl)methanone

Example 263((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 264((2S,3R)-3-((5-bromopyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(oxazol-2-yl)phenyl)methanone

Example 265((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

The title compound was prepared analogous to Example 14 substitutingintermediate A-11 with intermediate A-38. MS (ESI): mass calcd. forC₂₂H₂₁F₃N₆O, 442.2. m/z found, 443.2 [M+H]⁺. Analytical HPLC wasobtained on a Agilent 1100 Series using a XBridge C18 column (5 μm,100×4 6 mm), mobile phase of 10-100% ACN in 20 mM NH₄OH over 8 min andthen hold at 100% ACN for 3 min, at a flow rate of 1 mL/min(Temperature=30° C.). R_(t)=6.71 min at 280 nm.

Example 266((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 267((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(4-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 268((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 269((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 270((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(4-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone

Example 271((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6-(pyrimidin-2-yl)phenyl)methanone

Example 272(2-fluoro-6-(pyrimidin-2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone

Example 273((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6-(pyrimidin-2-yl)phenyl)methanone

Assays:

The in vitro affinity of the compounds of the invention for therat/human orexin 1 and human orexin 2 receptors was determined bycompetitive radioligand binding using[³H](1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)(Langmead et al., 2004) and [³H]EMPA(n-ethyl-2[96-methoxypyridin-3-yl)-(toluene-2-sulfonyl)-amino]-N-pyridin-3-ylmethylacetamide), respectively (Langmead et al., 2004, British Journal ofPharmacology 141:340-346; Malherbe et al., 2004, British Journal ofPharmacology 156:1326-41).

The in vitro functional antagonism of the compounds on the human orexin1 and orexin 2 receptors was determined using fluorometric imaging platereader (FLIPR) based calcium assays.

Data are analyzed using pc-Sandy macro and graphed on Graphpad Prism 5.For analysis, each concentration point is averaged from triplicatevalues and the averaged values are plotted on Graphpad Prism. The IC50was determined by applying the following equation (GraphPad Prism 5.0,SanDiego) for one site competition where X=log (concentration) andY=specific binding. Top denotes the total[³H]-(1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)binding, bottom denotes the nonspecific[³H]-(1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)binding. Graphpad Prism calculates Ki value from IC50 and thepre-determined Kd values for[³H]-(1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)and [3H]-EMPA. The Ki for each compound is then uploaded into 3DX. Eachrun comprises individual compounds in triplicate. The data in Table 1and Table 2 represent averages from between 2-20 runs.

Rat and Human Orexin 1 Receptor Radioligand Binding Studies

Human Embryonic Kidney 293 cells (HEK293) stably expressing rat orexin 1receptor (Genebank accession number NM_001525) or Chinese ovary cells(CHO) stably expressing human orexin 1 receptor (Genebank accessionnumber NM_001526) were grown to confluency in DMEM (Hyclone, cat#SH30022), 10% FBS, 1×Pen/Strep, 1× sodium pyruvate, 10 mM HEPES, 600μg/mL G418 and DMEM/F12 (Gibco, Cat #11039), 10% FBS, 1×Pen/Strep, 600μg/mL G418 media, respectively on 150 cm² tissue culture plates, washedwith 5 mM EDTA in PBS (HyClone Dulbecco's Phosphate Buffered Saline 1×with Calcium and Magnesium, Cat #SH30264.01, hereafter referred tosimply as PBS) and scraped into 50 ml tubes. After centrifugation (2K×G,5 min at 4° C.), the supernatant was aspirated and the pellets frozenand stored at −80° C. Cells were resuspended in PBS in the presence of 1tablet of protease inhibitor cocktail (Roche, Cat. #11836145001) per 50mL. Each cell pellet from a 15 cm plate was resuspended in 10 mL, storedon ice, and homogenized for 45 sec prior to addition to the reactions.Competition binding experiments in 96 well polypropylene plates wereperformed using[³H]-(1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)(Moraveck Corporation, specific activity=35.3 Ci/mmol), diluted to a 10nM concentration in PBS (4 nM final). Compounds were solubilized in 100%DMSO (Acros Organics, Cat. #61042-1000) and tested over a range of 7concentrations (from 0.1 nM to 10 μM). The final concentration of DMSOin the reactions is equal to or less than 0.1%. Total and nonspecificbinding was determined in the absence and presence of 10 μM almorexant.The total volume of each reaction is 200 μL (20 μL of diluted compounds,80 μL of[³H]-(1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone)diluted in PBS and 100 μL of the cell suspension). Reactions were runfor 60 min at room temperature and terminated by filtration through GF/Cfilter plates (PerkinElmer, Cat. #6005174) presoaked in 0.3%polyethylenimine using the cell harvester (PerkinElmer Filtermate). Theplates were washed 3 times by aspirating 30 ml PBS through the plates.Plates were dried in 55° C. oven for 60 min, scintillation fluid wasadded, and the radioactivity was counted on a Topcount (Packard).

IC₅₀ values (i.e. concentration of unlabelled compound required tocompete for 50% of specific binding to the radioligand) was calculatedusing the GraphPad Prism software (GraphPad Prism Software Inc., SanDiego, Calif.) with a fit to a sigmoidal dose-response curve. ApparentKi values were calculated as K_(i)=IC₅₀/(1+C/K_(d)), where C isconcentration of radioligand and K_(d)=4 nM for rat orexin 1 receptorand 6 nM for human orexin 1 receptor.

Human Orexin 2 Receptor Radioligand Binding Studies

HEK293 stably expressing human orexin 2 receptor (Genebank accessionnumber NM_001526) were grown to confluency in DMEM (Hyclone, cat#SH30022), 10% FBS, 1×Pen/Strep, 1× NaPyruvate, 10 mM HEPES, 600 ug/mlG418 media on 150 cm² tissue culture plates, washed with 5 mM EDTA inPBS (HyClone Dulbecco's Phosphate Buffered Saline 1× with Calcium andMagnesium, Cat #SH30264.01, hereafter referred to simply as PBS) andscraped into 50 ml tubes. After centrifugation (2K×G, 5 min at 4° C.),the supernatant was aspirated and the pellets frozen and stored at −80°C. Cells were resuspended in PBS in the presence of 1 tablet of proteaseinhibitor cocktail (Roche, Cat. #11836145001) per 50 mL. Each cellpellet from a 15 cm plate was resuspended in 10 mL, stored on ice, andhomogenized for 45 sec just prior to addition to the reactions.Competition binding experiments in 96 well polypropylene plates wereperformed using [³H]-EMPA (Moraveck Corporation, specific activity=29.6Ci/mmol), diluted to a 5 nM concentration in PBS (2 nM finalconcentration). Compounds were solubilized in 100% DMSO (Acros Organics,Cat. #61042-1000) and tested over a range of 7 concentration (from 0.1nM to 10 μM). The final concentration of DMSO in the reactions is equalto or less than 0.1%. Total and nonspecific binding was determined inthe absence and presence of 10 μM almorexant. The total volume of eachreaction is 200 μL (20 μL of diluted compounds, 80 μL of [³H]-EMPAdiluted in PBS and 100 μL of the cell suspension). Reactions were runfor 60 min at room temperature and terminated by filtration through GF/Cfilter plates (PerkinElmer, Cat. #6005174) presoaked in 0.3%polyethylenimine using the cell harvester (PerkinElmer Filtermate). Theplates were washed 3 times by aspirating 30 ml PBS through the plates.Plates were dried in 55° C. oven for 60 min, scintillation fluid wasadded, and the radioactivity was counted on a Topcount (Packard).

IC₅₀ values (i.e. concentration of unlabelled compound required tocompete for 50% of specific binding to the radioligand) was calculatedusing the GraphPad Prism software (GraphPad Prism Software Inc., SanDiego, Calif.) with a fit to a sigmoidal dose-response curve. ApparentKi values were calculated as K_(i)=IC₅₀/(1+C/K_(d)), where C isconcentration of radioligand and K_(d)=2 nM.

Human Orexin 1 Receptor Ca²⁺ Mobilization Assay

CHO cells stably transfected with the human orexin 1 receptor (Genebankaccession number NM_001526) were grown to confluency in DMEM/F12, 10%FBS, 1×pen-strep, 400 μg/ml G418. Cells were seeded on to 384-wellPackard viewplates at a density of 10,000 cells/well and incubatedovernight at 37° C., 5% CO2. The cells were dye-loaded with BD CalciumAssay kit (BD, cat #640178) in HBSS (Gibco, cat#14025-092) with 2.5 mMprobenecid and incubated at 37° C., 5% CO₂ for 45 min. Cells werepre-incubated with compounds (diluted in DMEM/F-12) for 15-30 minutesbefore agonist (orexin A, 10 nM) stimulation. Ligand-induced Ca²⁺release was measured using a Fluorometric Imaging Plate Reader (FLIPR,Molecular Devices, Sunnyvale, Calif.). Functional responses weremeasured as peak fluorescence intensity minus basal. The concentrationof agonist that produced a half-maximal response is represented by theEC₅₀ value. Antagonistic potency values were converted to apparentpK_(B) values using a modified Cheng-Prusoff correction. ApparentpK_(B)=−log IC₅₀/1+[conc agonist/EC₅₀].

Human Orexin 2 Receptor Ca²⁺ Mobilization Assay

PFSK-1 cells endogenously expressing the human orexin 2 receptor weregrown to confluency in RPMI1640 (Hyclone, cat#30027.02), 10% FBS,1×pen-strep. Cells were seeded on to 384-well Packard viewplates at adensity of 5,000 cells/well and incubated overnight at 37° C., 5% CO2.The cells were dye-loaded with BD Calcium Assay kit (BD, cat #640178) inHBSS (Gibco, cat#14025-092) with 2.5 mM probenecid and incubated at 37°C., 5% CO₂ for 45 min. Cells were pre-incubated with compounds (dilutedin DMEM/F-12) for 15-30 minutes before agonist (orexin B, 100 nM)stimulation. Ligand-induced Ca²⁺ release was measured using aFluorometric Imaging Plate Reader (FLIPR, Molecular Devices, Sunnyvale,Calif.). Functional responses were measured as peak fluorescenceintensity minus basal. The concentration of agonist that produced ahalf-maximal response is represented by the EC₅₀ value. Antagonisticpotency values were converted to apparent pK_(B) values using a modifiedCheng-Prusoff correction. Apparent pK_(B)=−log IC₅₀/1+[concagonist/EC₅₀].

Preferred compounds of the invention are set forth in Table 1 below.Orexin receptor activity of certain compounds of the invention is alsoset forth in the below table.

TABLE 1 rOX1 hOX1 hOX2 Ex. Structure K_(i) (nM) K_(i) (nM) K_(i) (nM)Compound Name  1

 1034   290 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone  2

  400   217 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(2-fluoro-6-(pyrimidin-2-yl)phenyl)methanone  3

 1065   260 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)methanone  4

  78   40 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((4,6-dimethylpyrimidin-2- yl)amino)-2-methylpiperidin-1- yl)methanone  5

  560   116 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone  6

  25   40   25 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-2-(pyrimidin-2-yl)phenyl)methanone  7

  126   228 (±)-trans-(5-methyl-2- (pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl-2- (trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1- yl)methanone  8

  462   587 (±)-trans-(5-methyl-2-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((6-methyl-2- (trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1- yl)methanone  9

 6708  2054 (±)-trans-(5-fluoro-2- (pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl-2- (trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1- yl)methanone  10

  315   144 (±)-trans-(2-fluoro-6-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((4- (trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone  11

 8999   634 (±)-trans-(6-methyl-2-(1H- 1,2,3-triazol-1-yl)pyridin-3-yl)(2-methyl-3-((4- (trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone  12

  14   11   332 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  13

  15    9   920 (±)-trans-(4-methyl-2-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone  14

   9    7   681 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  15

 8200 >10000 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2R,3S)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  16

  35   38   680 (±)-trans-(3-ethoxy-6- methylpyridin-2-yl)(2-methyl-3-((5-(trifluoromethyl)pyridin- 2-yl)amino)piperidin-1- yl)methanone  17

  11    8   221 (±)-trans-(5-chloro-2-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone  18

  13   10   272 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone  19

  10   10   238 (±)-trans-(5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)(2- methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  20

  14    8   150 (±)-trans-(3-methyl-2-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone  21

  19   25   595 (±)-trans-(3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  22

  33   19   422 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  23

  36   23   208 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone  24

   8   16   324 (±)-trans-(5-fluoro-2-(2H- 1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone  25

  11   19   190 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  26

  11   14  2035 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone  27

  19   26   91 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5- methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone  28

  496  3900 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone  29

  97   90  1400 (±)-trans-(2-ethoxyphenyl)(2- methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone  30

  55   54   656 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((5-chloropyrimidin-2-yl)amino)-2- methylpiperidin-1- yl)methanone  31

  131   120   508 (±)-trans-(2-(2H-1,2,3- 2-yl)phenyl)(2-methyl-3-((5-methylpyrimidin-2- yl)amino)piperidin-1- yl)methanone  32

  34   12   286 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((5-chloropyridin-2-yl)amino)-2- methylpiperidin-1- yl)methanone  33

  199   160  4800 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((6- (trifluoromethyl)pyridazin-3-yl)amino)piperidin-1- yl)methanone  34

  355   700 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)amino)-2- methylpiperidin-1- yl)methanone  35

  20   29   738 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1- yl)methanone  36

  690  1500 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(2-methyl-3-(pyridin-2-ylamino)piperidin-1- yl)methanone  37

  76   71   824 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- methylpyridin-2- yl)amino)piperidin-1-yl)methanone  38

  18   25   529 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1- yl)methanone  39

  32   12   189 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(2-methyl-3-(quinazolin-2- ylamino)piperidin-1- yl)methanone  40

  870   822 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((5-fluoropyrimidin-2-yl)amino)-2- methylpiperidin-1- yl)methanone  41

   9    9   284 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-(benzo[d]oxazol-2-ylamino)-2- methylpiperidin-1- yl)methanone  42

  11   10   410 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((5-bromopyridin-2-yl)amino)-2- methylpiperidin-1- yl)methanone  43

  715  4600 (±)-trans-(3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin- 1-yl)(3-fluoro-2- methoxyphenyl)methanone 44

  82   72  1600 (±)-trans-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin- 1-yl)(2- ethoxyphenyl)methanone  45

  28   26   281 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2- yl)amino)-2-methylpiperidin-1- yl)methanone  46

  185   153   489 (±)-trans-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1- yl)(2-ethoxyphenyl)methanone  47

  373  1300 (±)-trans-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1- yl)(3-fluoro-2- methoxyphenyl)methanone 48

  14   11   96 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(2-methyl-3-(quinoxalin-2- ylamino)piperidin-1- yl)methanone  49

  95   70   89 (±)-trans-((2-ethoxyphenyl)(2- methyl-3-(quinoxalin-2-ylamino)piperidin-1- yl)methanone  50

  396   353 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-(quinoxalin-2- ylamino)piperidin-1- yl)methanone  51

  341  2100 (±)-trans-(2-(2H-1,2,3-triazol- 2-yl)phenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1- yl)methanone  52

 6200  2700 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1- yl)methanone  53

  715   58 (±)-trans-(2-ethoxyphenyl)(2- methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1- yl)methanone  54

 4500 >10000 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone  55

 1200 >10000 (±)-cis-(2-ethoxyphenyl)(2- methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone  56

  128   112  3600 (±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  57

  720 >10000 (±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-bromopyridin- 2-yl)amino)-2-methylpiperidin-1-yl)methanone  58

>10000 >10000 (±)-cis-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(3-fluoro-2- methoxyphenyl)methanone 59

 1800 >10000 (±)-cis-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(2-ethoxyphenyl)methanone  60

>10000 >10000 (±)-cis-((2-(2H-1,2,3-triazol-2- yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2- yl)amino)-2-methylpiperidin-1- yl)methanone  61

 1100  2500 (±)-cis-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1- yl)(2-ethoxyphenyl)methanone  62

 1800  5700 (±)-cis-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1- yl)(3-fluoro-2- methoxyphenyl)methanone 63

 3300 >10000 (±)-cis-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-(quinoxalin-2- ylamino)piperidin-1- yl)methanone  64

 1100  1300 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-(quinoxalin-2- ylamino)piperidin-1- yl)methanone  65

  314   295 (±)-cis-(2-ethoxyphenyl)(2- methyl-3-(quinoxalin-2-ylamino)piperidin-1- yl)methanone  66

  570  1800 (±)-cis-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((4-phenylyrimidin-2- yl)amino)piperidin-1- yl)methanone  67

  840 >10000 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1- yl)methanone  68

 1400 >10000 (±)-cis-(2-ethoxyphenyl)(2- methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1- yl)methanone  69

  70   112 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1- yl)methanone  70

  170   165  7501 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1- yl)methanone  71

  10   12  1500 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl- 3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1- yl)methanone  72

  24   33  4025 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone  73

   7   21  1889 ((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1- yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2- yl)methanone  74

  40   52 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1- yl)methanone  75

  23   40  9499 ((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1- yl)(6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3- yl)methanone  76

  54   62  1300 ((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1- yl)(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3- yl)methanone  77

  36   55  4018 ((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  78

  30   57   436 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone  79

   8    7  1300 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1- yl)methanone  80

  29   24  4900 ((2S,3R)-3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  81

  14   23   100 ((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1- yl)(6-methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone  82

  53   58  6400 (4-methoxy-2-(2H-1,2,3- triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone  83

  57   53  7000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5- methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  84

  23   28   173 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone  85

   8   10   210 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1- yl)methanone  86

  213   249 >10000 ((2S,3R)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  87

   7   12   154 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone  88

  13   25  3300 ((2S,3R)-3-(benzo[d]oxazol-2-ylamino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone  89

  14   10   371 (5-methoxy-2-(2H-1,2,3- triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone  90

  20   19   486 (5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3- ((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1- yl)methanone  91

  32   48  2100 ((2S,3R)-3-((5- chloropyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4- methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone  92

  19   18  4200 ((2S,3R)-3-((5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2- yl)methanone  93

  52   38  2700 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  94

  38   25 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(5- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone  95

  81   103 >10000 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2- methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1- yl)methanone  96

  81   47  3900 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1- yl)methanone  97

  44   32  6100 (4-methoxy-2-(2H-1,2,3- triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone  98

  26   12 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2- methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1- yl)methanone  99

  23   15  1400 ((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 100

   8   12   207 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2- methylpiperidin-1- yl)methanone 101

  215   446 >10000 ((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2- yl)pyridin-2-yl)methanone 102

  28   25  5200 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl- 3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1- yl)methanone 103

  44   97  2300 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin- 2-yl)oxy)piperidin-1- yl)methanone 104

  119   173 >10000 ((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)(6-methyl-3-(2H-1,2,3-triazol-2- yl)pyridin-2-yl)methanone 105

((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(5- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 106

(2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1- yl)methanone 107

((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 108

((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 109

(±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 110

(2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 111

((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(5- methyl-3-(2H-1,2,4-triazol-2-yl)pyridin-2-yl)methanone 112

   8   12   562 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1- yl)methanone 113

(4-methyl-2-(2H-1,2,3-triazol- 2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin- 2-yl)oxy)piperidin-1- yl)methanone 114

(3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 115

(3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 116

(3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 117

(3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin- 2-yl)oxy)piperidin-1- yl)methanone 118

(3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1- yl)methanone 119

((2S,3R)-3-(benzo[d]oxazol-2- yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(pyrimidin-2- yl)phenyl)methanone 120

((2S,3R)-3-(benzo[d]oxazol-2- yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(2H-1,2,3- triazol-2-yl)phenyl)methanone 121

(3-fluoro-2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 122

(3-fluoro-2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 123

(3-fluoro-2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin- 2-yl)oxy)piperidin-1- yl)methanone 124

(3-fluoro-2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 125

(3-fluoro-2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1- yl)methanone 126

((2S,3R)-3-(benzo[d]oxazol-2- yloxy)-2-methylpiperidin-1-yl)(3-fluoro-2-(2H-1,2,3- triazol-2-yl)phenyl)methanone 127

((2S,3R)-3-(benzo[d]oxazol-2- yloxy)-2-methylpiperidin-1-yl)(2-fluoro-6-(2H-1,2,3- triazol-2-yl)phenyl)methanone 128

(2-fluoro-6-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 129

(2-fluoro-6-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 130

(2-fluoro-6-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin- 2-yl)oxy)piperidin-1- yl)methanone 131

(2-fluoro-6-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 132

(2-fluoro-6-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-ethyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1- yl)methanone 133

(2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1- yl)methanone 134

(2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 135

(2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin- 2-yl)oxy)piperidin-1- yl)methanone 136

(2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((5-fluoropyrimidin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 137

(2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 138

(5-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 139

(4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 140

(4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1- yl)methanone 141

(4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)oxy)-2- methylpiperidin-1- yl)methanone 142

(3-fluoro-2-(5-fluoropyrimidin- 2-yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin- 2-yl)oxy)piperidin-1- yl)methanone 143

((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(2- (pyrimidin-2- yl)phenyl)methanone 144

(2-(3-methyl-1,2,4-oxadiazol-5- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone 145

(4-fluoro-2-(3-methyl-1,2,4- oxadiazol-5- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1- yl)methanone 146

(3-fluoro-2-(3-methyl-1,2,4- oxadiazol-5- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1- yl)methanone

Preferred compounds of the invention are set forth in Table 2 below.Data for Orexin receptor activity additional testing of certaincompounds of the invention is also set forth in Table 2.

TABLE 2 rOX1 hOX1 hOX2 K_(i) K_(i) K_(i) Ex. Structure (nM) (nM) (nM)Compound Name 1

1034 290 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(4-fluoro-2- (2H-1,2,3-triazol-2-yl)phenyl)methanone 2

400 217 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6- (pyrimidin-2-yl)phenyl)methanone 3

1065 260 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(2-fluoro-6- (2H-1,2,3-triazol-2-yl)phenyl)methanone 4

78 40 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(3-((4,6-dimethylpyrimidin-2-yl)amino)-2- methylpiperidin-1-yl)methanone 5

560 116 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(5-fluoro-2- (2H-1,2,3-triazol-2-yl)phenyl)methanone 6

25 40 27 (±)-trans-(3-((4,6- dimethylpyrimidin-2-yl)amino)-2-methylpiperidin-1-yl)(5-methyl-2- (pyrimidin-2-yl)phenyl)methanone 7

127 243 (±)-trans-(5-methyl-2-(pyrimidin- 2-yl)phenyl)(2-methyl-3-((6-methyl-2- (trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone 8

466 590 (±)-trans-(5-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((6-methyl-2-(trifluoromethyl)pyrimidin-4- yl)amino)piperidin-1-yl)methanone 9

7000 2256 (±)-trans-(5-fluoro-2-(pyrimidin-2-yl)phenyl)(2-methyl-3-((6-methyl- 2-(trifluoromethyl)pyrimidin-4-yl)amino)piperidin-1-yl)methanone 10

315 144 (±)-trans-(2-fluoro-6-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((4-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 11

9000 634 (±)-trans-(6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)(2-methyl- 3-((4-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 12

16 16 437 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 13

16 9 923 (±)-trans-(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 14

9 7 687 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 15

8200 >10000 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2R,3S)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 16

35 38 680 (±)-trans-(3-ethoxy-6- methylpyridin-2-yl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 17

11 8 221 (±)-trans-(5-chloro-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 18

13 10 272 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 19

10 10 239 (±)-trans-(5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 20

14 8 150 (±)-trans-(3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 21

19 25 595 (±)-trans-(3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 22

36 21 422 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(oxazol-2-yl)pyridin-2-yl)methanone 23

36 23 208 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(pyrimidin-2-yl)pyridin-2-yl)methanone 24

8 16 324 (±)-trans-(5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 25

12 19 191 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(oxazol-2-yl)pyridin-2-yl)methanone 26

12 16 2367 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 27

19 26 91 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(5-methyl- 3-(pyrimidin-2-yl)pyridin-2-yl)methanone 28

496 3900 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 29

97 90 1400 (±)-trans-(2-ethoxyphenyl)(2- methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 30

55 54 656 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-chloropyrimidin- 2-yl)amino)-2-methylpiperidin-1-yl)methanone 31

131 120 508 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-methylpyrimidin-2- yl)amino)piperidin-1-yl)methanone 32

34 12 286 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-chloropyridin-2- yl)amino)-2-methylpiperidin-1-yl)methanone 33

199 160 4800 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-((6- (trifluoromethyl)pyridazin-3-yl)amino)piperidin-1-yl)methanone 34

355 700 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-fluoropyridin-2-yl)amino)-2- methylpiperidin-1-yl)methanone 35

20 29 738 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone 36

690 1500 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(pyridin-2- ylamino)piperidin-1-yl)methanone 37

76 71 824 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-methylpyridin-2- yl)amino)piperidin-1-yl)methanone 38

18 25 529 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone 39

32 12 189 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinazolin- 2-ylamino)piperidin-1- yl)methanone40

870 822 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-fluoropyrimidin- 2-yl)amino)-2-methylpiperidin-1-yl)methanone 41

9 9 284 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)methanone 42

11 10 410 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)methanone 43

715 4600 (±)-trans-(3-((5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2- methoxyphenyl)methanone 44

82 72 1600 (±)-trans-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(2-ethoxyphenyl)methanone 45

28 26 281 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2-yl)amino)- 2-methylpiperidin-1-yl)methanone 46

185 153 489 (±)-trans-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(2- ethoxyphenyl)methanone 47

373 1300 (±)-trans-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2- methoxyphenyl)methanone 48

14 11 96 (±)-trans-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinoxalin- 2-ylamino)piperidin-1- yl)methanone49

95 70 89 (±)-trans-((2-ethoxyphenyl)(2- methyl-3-(quinoxalin-2-ylamino)piperidin-1-yl)methanone 50

396 353 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1- yl)methanone 51

341 2100 (±)-trans-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1-yl)methanone 52

6200 2700 (±)-trans-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1-yl)methanone 53

715 58 (±)-trans-(2-ethoxyphenyl)(2- methyl-3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone 54

4500 >10000 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 55

1200 >10000 (±)-cis-(2-ethoxyphenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 56

128 112 3600 (±)-cis-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 57

720 >10000 (±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(3-((5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)methanone 58

>10000 >10000 (±)-cis-(3-(5-bromopyridin-2-yl)amino)-2-methylpiperidin-1- yl)(3-fluoro-2- methoxyphenyl)methanone59

1800 >10000 (±)-cis-(3-(5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)(2-ethoxyphenyl)methanone 60

>10000 >10000 (±)-cis-((2-(2H-1,2,3-triazol-2- yl)phenyl)(3-((5-chlorobenzo[d]oxazol-2-yl)amino)- 2-methylpiperidin-1-yl)methanone 61

1100 2500 (±)-cis-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(2- ethoxyphenyl)methanone 62

1800 5700 (±)-cis-(3-((5- chlorobenzo[d]oxazol-2-yl)amino)-2-methylpiperidin-1-yl)(3-fluoro-2- methoxyphenyl)methanone 63

3300 >10000 (±)-cis-(2-(2H-1,2,3-triazol-2-yl)phenyl)(2-methyl-3-(quinoxalin- 2-ylamino)piperidin-1- yl)methanone64

1100 1300 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin-1- yl)methanone 65

314 295 (±)-cis-(2-ethoxyphenyl)(2-methyl-3-(quinoxalin-2-ylamino)piperidin- 1-yl)methanone 66

570 1800 (±)-cis-(2-(2H-1,2,3-triazol-2- yl)phenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1-yl)methanone 67

840 >10000 (±)-cis-(3-fluoro-2- methoxyphenyl)(2-methyl-3-((4-phenylpyrimidin-2- yl)amino)piperidin-1-yl)methanone 68

1400 >10000 (±)-cis-(2-ethoxyphenyl)(2-methyl- 3-((4-phenylpyrimidin-2-yl)amino)piperidin-1-yl)methanone 69

70 112 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 70

170 165 7500 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 71

10 12 1500 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 72

24 33 4050 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 73

8 22 2267 ((2S,3R)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2- yl)pyridin-2-yl)methanone 74

40 52 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 75

22 42 8950 ((2S,3R)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(6-methyl-2-(2H-1,2,3-triazol-2- yl)pyridin-3-yl)methanone 76

54 62 1300 ((2S,3R)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(6-methyl-2-(1H-1,2,3-triazol-1- yl)pyridin-3-yl)methanone 77

41 60 4233 ((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone 78

30 57 436 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(pyrimidin-2-yl)pyridin-2-yl)methanone 79

8 7 1300 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 80

29 24 4900 ((2S,3R)-3((5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2- yl)pyridin-2-yl)methanone 81

14 23 100 ((2S,3R)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(6-methyl-3-(pyrimidin-2- yl)pyridin-2-yl)methanone 82

53 58 6400 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 83

57 53 7000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(5-methyl- 3-(oxazol-2-yl)pyridin-2-yl)methanone 84

23 28 173 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(pyrimidin-2-yl)pyridin-2-yl)methanone 85

8 10 210 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone 86

213 249 >10000 ((2S,3R)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(oxazol-2-yl)pyridin-2-yl)methanone 87

7 12 154 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(6-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 88

13 25 3300 ((2S,3R)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(5-methyl-3-(oxazol-2- yl)pyridin-2-yl)methanone 89

11 9 422 (5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 90

20 19 486 (5-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 91

32 48 2100 ((2S,3R)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 92

19 18 4200 ((2S,3R)-3-((5-bromopyridin-2- yl)amino)-2-methylpiperidin-1-yl)(5-methyl-3-(2H-1,2,3-triazol-2- yl)pyridin-2-yl)methanone 93

52 38 2700 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 94

38 25 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)(5-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 95

81 103 >10000 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 96

81 47 3900 (6-methyl-2-(1H-1,2,3-triazol-1-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 97

33 34 4900 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 98

24 21 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 99

23 15 1400 ((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 100

6 9 190 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((5-chloropyridin-2-yl)amino)-2- methylpiperidin-1-yl)methanone 101

215 446 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(5-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 102

28 25 5200 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone 103

44 97 2300 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)methanone 104

119 173 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(6-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 105

192 269 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(5-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 106

44 45 912 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1-yl)methanone 107

34 38 2300 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(6-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 108

9 7 301 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 109

18 22 463 (±)-trans-(2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 110

12 11 246 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 111

22 31 5100 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(5-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 112

7 11 664 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 113

25 43 3900 (4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone 116

15 18 598 (3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 124

21 13 460 (3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 125

117 138 2500 (3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone 138

20 18 544 (5-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 139

51 51 1200 (4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 141

31 22 676 (2-(5-fluoropyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 143

25 15 473 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(2- (pyrimidin-2-yl)phenyl)methanone 147

50 31 765 (4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 148

3400 >10000 (2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone 149

24 18 5600 (5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 150

32 39 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 151

35 31 537 (3-methyl-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 152

28 20 412 (3-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 153

353 >10000 (2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 154

46 22 526 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2- (pyrimidin-2-yl)phenyl)methanone 155

28 16 1100 (3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)- 2-methylpiperidin-1-yl)methanone 156

47 30 1300 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(2-(5- fluoropyrimidin-2- yl)phenyl)methanone 157

49 27 >10000 ((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)- 2-methylpiperidin-1-yl)(5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3- yl)methanone 158

36 34 575 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl- 2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 159

60 14 1550 (3-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)methanone 160

23 22 620 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl- 3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 161

226 395 3200 ((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(3- fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 162

621 >10000 ((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(5- methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone 163

100 224 1050 ((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(3- methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 164

144 230 3800 ((2S,3R)-3-((5-chloropyrimidin-2-yl)oxy)-2-methylpiperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 165

59 77 4400 (2-(5-fluoropyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)methanone 166

97 124 3100 (2-(5-fluoropyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1-yl)methanone 167

30 28 1800 (3-methyl-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)methanone 168

29 25 951 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(2- (pyrimidin-2-yl)phenyl)methanone 169

32 23 1200 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(2- (pyrimidin-2-yl)phenyl)methanone 170

48 49 2800 (2-(5-fluoropyrimidin-2-yl)-3-methylphenyl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone 171

34 29 1100 (3-methyl-2-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1-yl)methanone 172

447 >10000 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone 173

423 8200 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)(3- phenylpyrazin-2-yl)methanone 174

504 >10000 (4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone 175

124 188 4400 (5-fluoro-2-(oxazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)oxy)piperidin-1-yl)methanone 176

374 >10000 (5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)methanone 177

238 166 >10000 (5-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyrazin-2-yl)oxy)piperidin-1-yl)methanone 178

679 >10000 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)oxy)piperidin-1-yl)(3- phenylpyrazin-2-yl)methanone 179

100 68 1900 (4-methoxy-2-(oxazol-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)oxy)piperidin-1-yl)methanone 180

31 27 1900 ((2S*,3R*)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 181

93 176 5200 ((2S*,3R*)-3-((5-chloropyridin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 182

93 148 10000 ((2S*,3R*)-3((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 183

103 224 8900 ((2S*,3R*)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(5-methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 184

7 6 191 (5-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3- ((5-(trifluoromethyl)pyrazin-2-yl)amino)piperidin-1-yl)methanone 185

259 8600 ((2S*,3R*)-3-((5-chloropyrimidin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 186

278 9400 ((2S*,3R*)-3-((5-chloropyrazin-2-yl)amino)-2-methylpiperidin-1- yl)(4-methoxy-2-(2H-1,2,3-triazol-2-yl)phenyl)methanone 187

20 39 2500 ((2S*,3R*)-3-(benzo[d]oxazol-2- yl(methyl)amino)-2-methylpiperidin-1-yl)(5-methyl-3- (2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 188

92 100 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S*,3R*)-2- methyl-3-(methyl(5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 189

7 5 175 (3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 190

26 18 1200 (4-fluoro-2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl)((2S*,3R*)- 2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 191

12 9 421 (4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 192

27 25 543 (4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3- ((5-(trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)methanone 193

1100 >10000 (2-chloro-6-methoxypyridin-3- yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 194

24 24 315 (3-ethoxy-6-methylpyridin-2- yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 195

357 10000 ((2S*,3R*)-2-methyl-3-((5- (trifluoromethyl)-1,3,4-thiadiazol-2-yl)amino)piperidin-1-yl)(5- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 196

1800 >10000 (6-methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)((2S*,3R*)-2- methyl-3-((5-(trifluoromethyl)-1,3,4-thiadiazol-2- yl)amino)piperidin-1-yl)methanone 197

332 6600 (4-fluoro-2-(3-methyl-1,2,4- oxadiazol-5-yl)phenyl)((2S*,3R*)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 198

119 92 2500 (4-fluoro-2-(2H-1,2,3-triazol-2-yl)phenyl)((2S*,3R*)-2-methyl-3- ((5-(trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 199

154 206 2400 (3-ethoxy-6-methylpyridin-2- yl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone 200

35 27 985 (3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone 201

>10000 >10000 (2-chloro-6-methoxypyridin-3-yl)((2S*,3R*)-2-methyl-3-((5- (trifluoromethyl)pyrimidin-2-yl)amino)piperidin-1-yl)methanone 202

35 65 1500 (4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone 203

34 28 755 (4-fluoro-2-(oxazol-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)amino)piperidin-1-yl)methanone 204

14 16 292 ((2S*,3R*)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(3-fluoro-2-(pyrimidin-2- yl)phenyl)methanone 205

411 5800 (4-fluoro-2-(oxazol-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrimidin-2- yl)amino)piperidin-1-yl)methanone 206

16 16 564 ((2S*,3R*)-3-(benzo[d]oxazol-2- ylamino)-2-methylpiperidin-1-yl)(4-fluoro-2-(pyrimidin-2- yl)phenyl)methanone 207

15 12 385 (3-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone 208

67 34 1100 (4-fluoro-2-(oxazol-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone 209

16 25 944 (4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S*,3R*)-2-methyl-3-((5-(trifluoromethyl)pyrazin-2- yl)amino)piperidin-1-yl)methanone 210

25 32 742 ((2S*,3R*)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(3- (pyrimidin-2-yl)pyridin-2- yl)methanone 211

120 124 >10000 (5-methyl-2-(pyrimidin-2-yl)pyridin-3-yl)((2S,3R)-2-methyl- 3-((5-(trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)methanone 212

45 132 915 (2-fluoro-6-(pyrimidin-2- yl)phenyl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 213

23 68 217 ((2S,3R)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)oxy)piperidin-1-yl)(6-methyl-3- (pyrimidin-2-yl)pyridin-2-yl)methanone 214

38 78 5400 ((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5- methyl-2-(2H-1,2,3-triazol-2-yl)pyridin-3-yl)methanone 215

12 35 374 ((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6- methyl-3-(2H-1,2,3-triazol-2-yl)pyridin-2-yl)methanone 216

52 89 8200 ((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5- methyl-3-(pyrimidin-2-yl)pyridin-2-yl)methanone 217

177 154 8900 ((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(5- methyl-2-(pyrimidin-2-yl)pyridin-3-yl)methanone 218

26 29 495 ((2S,3R)-3-((5-chloropyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3- (pyrimidin-2-yl)pyridin-2-yl)methanone 219

51 55 1300 (3-ethoxy-6-methylpyridin-2- yl)((2S,3R)-2-methyl-3-((5-(trifluoromethyl)pyridin-2- yl)oxy)piperidin-1-yl)methanone 220

23 25 393 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(3-methyl- 2-(pyrimidin-2- yl)phenyl)methanone221

32 44 533 (5-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)- 2-methylpiperidin-1-yl)methanone 222

55 91 1300 (4-fluoro-2-(pyrimidin-2- yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)- 2-methylpiperidin-1-yl)methanone 223

35 45 370 ((2S,3R)-3-((3-fluoro-5- (trifluoromethyl)pyridin-2-yl)oxy)-2-methylpiperidin-1-yl)(6-methyl- 3-(pyrimidin-2-yl)pyridin-2-yl)methanone 224

33 47 576 (2-(2H-1,2,3-triazol-2- yl)phenyl)((2S,3R)-3-((3-fluoro-5-(trifluoromethyl)pyridin-2-yl)oxy)- 2-methylpiperidin-1-yl)methanone 265

16 24 293 ((2S*,3R*)-2-methyl-3-((5- (trifluoromethyl)pyridin-2-yl)amino)piperidin-1-yl)(3- (pyrimidin-2-yl)pyridin-2- yl)methanone

What is claimed:
 1. A compound of Formula I:

or an enantiomer, diastereomer, tautomer, or isotopic variant thereof;or a pharmaceutically acceptable salt or solvate thereof; wherein X isCR₁ or N; Y is CR₂ or N; Z is NH, N—CH₃, or O; R₁ is H, alkoxy,triazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl, whereintriazolyl, oxazolyl, isoxazolyl, oxadiazolyl, or pyrimidinyl isoptionally substituted with up to two substituents selected from halo oralkyl; R₂ is H, alkyl, or halo; R₃ is H, alkyl, alkoxy, halo, triazolyl,oxazolyl, or pyrimidinyl, wherein triazolyl, oxazolyl, or pyrimidinyl isoptionally substituted with up to two substituents selected from halo oralkyl; R₄ is alkyl; R₅ is pyridyl, benzoxazolyl, pyrimidinyl,pyridazinyl, quinoxalinyl, pyrazinyl, thiadiazolyl, or quinazolinyl;wherein the pyridyl, benzoxazolyl, pyrimidinyl, pyridazinyl,quinoxalinyl, pyrazinyl, thiadiazolyl, or quinazolinyl is optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, and phenyl; and R₆ is H or alkyl. 2.The compound of claim 1, wherein Z is NH.
 3. The compound of claim 1wherein Z is O.
 4. The compound of claim 1, wherein R₄ is C₁₋₃alkyl. 5.The compound of claim 4, wherein R₄ is methyl.
 6. The compound of claim1, wherein X is CR₁ and Y is CR₂.
 7. The compound of claim 1, wherein Xis N and Y is CR₂.
 8. The compound of claim 1, wherein X is CR₁ and Y isN.
 9. The compound of claim 1, wherein X and Y are each N.
 10. Thecompound of claim 1, wherein R₁ is alkoxy, triazolyl, or pyrimidinyl.11. The compound of claim 10, wherein R₁ is alkoxy.
 12. The compound ofclaim 10, wherein R₁ is triazolyl.
 13. The compound of claim 10, whereinR₁ is pyrimidinyl.
 14. The compound of claim 13, wherein the pyrimidinylis halo-pyrimidinyl.
 15. The compound of claim 1, wherein R₁ isoxazolyl, isoxazolyl, or oxadiazolyl.
 16. The compound of claim 15,wherein oxadiazolyl is methyl-oxadiazolyl.
 17. The compound of claim 1,wherein R₂ is H.
 18. The compound of claim 1, wherein R₂ is halo. 19.The compound of claim 18, wherein halo is F, Cl, or Br.
 20. The compoundof claim 1, wherein R₂ is alkyl.
 21. The compound of claim 20, whereinalkyl is —CH₃.
 22. The compound of claim 1, wherein R₃ is alkyl, alkoxy,halo, triazolyl, oxazolyl, or pyrimidinyl.
 23. The compound of claim 22,wherein R₃ is alkyl.
 24. The compound of claim 23, wherein alkyl istrihaloalkyl.
 25. The compound of claim 23, wherein alkyl is —CH₃. 26.The compound of claim 22, wherein R₃ is alkoxy.
 27. The compound ofclaim 22, wherein R₃ is halo.
 28. The compound of claim 22, wherein R₃is triazolyl.
 29. The compound of claim 22, wherein R₃ is oxazolyl. 30.The compound of claim 22, wherein R₃ is pyrimidinyl.
 31. The compound ofclaim 1, wherein R₅ is pyridyl, optionally substituted with one or twosubstituents independently selected from the group consisting of alkyl,halo, and phenyl.
 32. The compound of claim 31, wherein alkyl istrihaloalkyl.
 33. The compound of claim 31, wherein alkyl is —CH₃. 34.The compound of claim 1 wherein R₅ is benzoxazolyl, optionallysubstituted with one or two substituents independently selected from thegroup consisting of alkyl, halo, and phenyl.
 35. The compound of claim34, wherein alkyl is trihaloalkyl.
 36. The compound of claim 34, whereinalkyl is —CH₃.
 37. The compound of claim 1, wherein R₅ is pyrimidinyl,optionally substituted with one or two substituents independentlyselected from the group consisting of alkyl, halo, and phenyl.
 38. Thecompound of claim 37, wherein alkyl is trihaloalkyl.
 39. The compound ofclaim 37, wherein alkyl is —CH₃.
 40. The compound of claim 1, wherein R₅is pyridazinyl, optionally substituted with one or two substituentsindependently selected from the group consisting of alkyl, halo, andphenyl.
 41. The compound of claim 40, wherein alkyl is trihaloalkyl. 42.The compound of claim 40, wherein alkyl is —CH₃.
 43. The compound ofclaim 1, wherein R₅ is quinoxalinyl, optionally substituted with one ortwo substituents independently selected from the group consisting ofalkyl, halo, and phenyl.
 44. The compound of claim 43, wherein alkyl istrihaloalkyl.
 45. The compound of claim 43, wherein alkyl is —CH₃. 46.The compound of claim 1, wherein R₅ is pyrazinyl, optionally substitutedwith one or two substituents independently selected from the groupconsisting of alkyl, halo, and phenyl.
 47. The compound of claim 46,wherein alkyl is trihaloalkyl.
 48. The compound of claim 46, whereinalkyl is —CH₃.
 49. The compound of claim 1, wherein R₅ is quinazolinyl,optionally substituted with one or two substituents independentlyselected from the group consisting of alkyl, halo, and phenyl.
 50. Thecompound of claim 49, wherein alkyl is trihaloalkyl.
 51. The compound ofclaim 49, wherein alkyl is —CH₃.
 52. The compound of claim 1, wherein R₆is H.
 53. The compound of claim 1, wherein R₆ is alkyl.
 54. A compoundselected from the group consisting of


55. The compound of claim 1 which is

or a pharmaceutically acceptable salt, solvate, isotopic variant,enantiomer, diastereomer, or tautomer thereof.
 56. The compound of claim55 which is


57. The compound of claim 1 which is

or a pharmaceutically acceptable salt, solvate, isotopic variant,enantiomer, diastereomer, or tautomer thereof.
 58. The compound of claim57 which is


59. The compound of claim 1 which is

or a pharmaceutically acceptable salt, solvate, isotopic variant,enantiomer, diastereomer, or tautomer thereof.
 60. The compound of claim59 which is


61. The compound of claim 1 which is

or a pharmaceutically acceptable salt, solvate, isotopic variant,enantiomer, diastereomer, or tautomer thereof.
 62. The compound of claim61 which is


63. The compound of claim 1 which is

or a pharmaceutically acceptable salt, solvate, isotopic variant,enantiomer, diastereomer, or tautomer thereof.
 64. The compound of claim63 which is


65. A pharmaceutical composition comprising a therapeutically effectiveamount of a compound of claim 1 and at least one pharmaceuticallyacceptable excipient.
 66. A method of treating a subject suffering fromor diagnosed with a disease, disorder, or medical condition mediated byorexin receptor activity, comprising administering to the subject aneffective amount of a compound of claim 1; wherein said disease,disorder, or medical condition is a sleep disorder, a metabolicdisorder, a neurological disorder, arrhythmia, acute heart failure,ulcer, irritable bowel syndrome, diarrhea, gastroesophageal reflux, mooddisorder, post-traumatic stress disorder, panic disorder, attentiondeficit disorder, cognitive deficiency, or substance abuse.
 67. Themethod of claim 66, wherein the sleep disorder is a sleep-waketransition disorder, insomnia, restless legs syndrome, jet-lag,disturbed sleep, or a sleep disorder secondary to a neurologicaldisorder.
 68. The method of claim 66, wherein the disease, disorder, ormedical condition is a mood disorder, post-traumatic stress disorder,panic disorder, attention deficit disorder, cognitive deficiency, orsubstance abuse.
 69. The method of claim 66, wherein the metabolicdisorder is overweight, obesity, insulin resistance, type II diabetes,hyperlipidemia, gallstones, angina, hypertension, breathlessness,tachycardia, infertility, sleep apnea, back and joint pain, varicoseveins, or osteoarthritis.
 70. The method of claim 66, wherein theneurological disorder is Parkinson's disease, Alzheimer's disease,Tourette's syndrome, catatonia, anxiety, delirium, or dementia.